Effects of cold-shock triploidy induction treatment on cell cleavage, hatching rate, and survival of loid larvae of spotted sand bass (Paralabrax maculatofasciatus)
Three experiments assessed the effects of cold-shock induced triploidy on (1) the incidence of abnormal cell cleavage during embryogenesis, (2) hatching success and the propor tion of live yolk-sac larv ae in normally cleaved versus abnormally clea ved embryos, and (3) survival of triploid larvae of...
| Autores: | , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Recursos: | Instituto Politécnico Nacional |
| Repositorio: | Redalyc-IPN |
| OAI Identifier: | oai:redalyc.org:48046495003 |
| Acesso em linha: | https://www.redalyc.org/articulo.oa?id=48046495003 |
| Access Level: | acceso abierto |
| Palavra-chave: | Ciencias de la Tierra triploidy Cell cleavage larval mortality embryoni c abnormalities Paralabrax maculatofasciatus |
| Resumo: | Three experiments assessed the effects of cold-shock induced triploidy on (1) the incidence of abnormal cell cleavage during embryogenesis, (2) hatching success and the propor tion of live yolk-sac larv ae in normally cleaved versus abnormally clea ved embryos, and (3) survival of triploid larvae of spotted sand bass ( Paralabrax maculatofasciatus ). All spawns were obtaine d by hormonal induction (LHRHa). Triploidy was induced by cold shock shortly after fertilizati on. In experiment 1, the percenta ge of abnormal ce ll cleavage was significantly higher ( P < 0.05) in the cold-shocked group than in the control group, whil e the hatching rate and the prop ortion of live yolk-sac larva e were significantly lower ( P < 0.05). In experiment 2, when norm ally and abnormally cleave d embryos from the control and cold-shocked groups were incubated separa tely, normal cleaved embryos in the cold-shocked group showed a significa ntly higher hatching rate and pro portion of live yolk-sac larvae than abnorm ally cleaved embryos in the same group. No difference was observed in the control group. In exp eriment 3, a significant decrease ( P < 0.05) in the triploidy count was observed throughout the larv al period. Final survival was drastically lower for triploid larvae compared to cold-shocked diploids and untreated eggs. Low triploidy percentage s at the end of the larval period would se riously hinder the development of triploid sand bass culture. |
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