Cloning, characterization, and functional expression of Taenia solium calreticulin
Calreticulin is an endoplasmic reticulum protein involved in the homeostasis of intracellular Call and other physiological processes. A complementary DNA clone containing the complete coding sequence of Taenia solium calreticulin (TsCRT) was isolated and characterized. Recombinant TsCRT was expresse...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2004 |
| País: | México |
| Institución: | Universidad Nacional Autónoma de México |
| Repositorio: | Sistema de Información de la Facultad de Ciencias, UNAM |
| OAI Identifier: | oai:repositorio.fciencias.unam.mx:11154/174349 |
| Acceso en línea: | http://hdl.handle.net/11154/99843 http://hdl.handle.net/11154/174349 |
| Access Level: | acceso abierto |
| Palabra clave: | 916 |
| Sumario: | Calreticulin is an endoplasmic reticulum protein involved in the homeostasis of intracellular Call and other physiological processes. A complementary DNA clone containing the complete coding sequence of Taenia solium calreticulin (TsCRT) was isolated and characterized. Recombinant TsCRT was expressed in bacteria as a 50-kDa protein that specifically bound calcium when tested in a radioassay. The deduced amino acid sequence has 47-50% identity with other reported calreticulins. Poor recognition of TsCRT by human and pig sera with confirmed cysticercosis discourages its use for diagnosis of the disease. However. further characterization and localization studies could provide insights into the role of TsCRT in T. solium physiology and host-parasite interactions. |
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