Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats

To improve on the efficiency of Quality Protein Maize (QPM) breeding in Uganada, the utility of three simple sequence repeats (SSR) markers (phi057, phi112 and umc1066) in selection and introgression of the opaque-2 (o2) gene was investigated. Genomic DNA of six normal and seven (QPM) lines was anal...

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Autores: Manna, R., Okello, D.K., Imanywoha, J., Pixley, K.V., Edema, R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:México
Institución:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/3028
Acceso en línea:http://hdl.handle.net/10883/3028
Access Level:acceso abierto
Palabra clave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
QPM
PROTEIN QUALITY
MAIZE
TRYPTOPHAN
ZEA MAYS
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spelling Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeatsManna, R.Okello, D.K.Imanywoha, J.Pixley, K.V.Edema, R.AGRICULTURAL SCIENCES AND BIOTECHNOLOGYQPMPROTEIN QUALITYMAIZETRYPTOPHANZEA MAYSTo improve on the efficiency of Quality Protein Maize (QPM) breeding in Uganada, the utility of three simple sequence repeats (SSR) markers (phi057, phi112 and umc1066) in selection and introgression of the opaque-2 (o2) gene was investigated. Genomic DNA of six normal and seven (QPM) lines was analysed using a standard PCR protocol. Polymorphisms were detected in the Opaque-2 locus among all the maize lines when using the SSR primers, phi057 and phi112, while no polymorphism was detected with primer umc1066. To facilitate background selection in the heterozygous BC2 progeny, key phenotypic characters of the recurrent parent (136R) were used as additional markers. The SSR marker phi057 was co-dominant while phi112 was dominant. The polymorphic SSR markers correctly predicted the expression of tryptophan in kernels of all QPM inbreds and five of the six non-QPM inbred lines. However, one non-QPM inbred line (PED49B) had a tryptophan content that is characteristic of the o2 mutation (0.090), suggesting that another genetic system may be responsible for the expression of tryptophan in this maize line. Phi057 was employed to monitor the introgression of the o2 allele from CML176 to 136R. Of the 200 BC1F1 (136R/CML176/136R) plants genotyped, 104 were found to be heterozygous, producing products corresponding to both alleles (o2 and O2), while 96 produced a single band corresponding to the homozygous dominant (O2O2) condition. The ratio of the two groups in the backcross (BC) population was consistent with proportion 1 :1 accorded by the Chi-square rest (X2=0.16<X2 0.05=3.84) for a single gene in a BC population. Therefore, under the conditions of PCR used, SSR markers phi057 and phi112 will constitute the framework for marker assisted introgression of the o2 trait into suitable maize genotypes in Uganda.215-226African Crop Science Societyhttp://www.bioline.org.br/abstract?id=cs05022&lang=en2013-06-30T05:24:27Z2013-06-30T05:24:27Z2005info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePDFapplication/pdfhttp://hdl.handle.net/10883/3028413African Crop Science Journalreponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.Open Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/30282024-10-11T19:58:50Z
dc.title.none.fl_str_mv Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
title Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
spellingShingle Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
Manna, R.
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
QPM
PROTEIN QUALITY
MAIZE
TRYPTOPHAN
ZEA MAYS
title_short Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
title_full Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
title_fullStr Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
title_full_unstemmed Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
title_sort Enhancing introgression of the Opaque-2 trait into elite maize lines using simple sequence repeats
dc.creator.none.fl_str_mv Manna, R.
Okello, D.K.
Imanywoha, J.
Pixley, K.V.
Edema, R.
author Manna, R.
author_facet Manna, R.
Okello, D.K.
Imanywoha, J.
Pixley, K.V.
Edema, R.
author_role author
author2 Okello, D.K.
Imanywoha, J.
Pixley, K.V.
Edema, R.
author2_role author
author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
QPM
PROTEIN QUALITY
MAIZE
TRYPTOPHAN
ZEA MAYS
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
QPM
PROTEIN QUALITY
MAIZE
TRYPTOPHAN
ZEA MAYS
description To improve on the efficiency of Quality Protein Maize (QPM) breeding in Uganada, the utility of three simple sequence repeats (SSR) markers (phi057, phi112 and umc1066) in selection and introgression of the opaque-2 (o2) gene was investigated. Genomic DNA of six normal and seven (QPM) lines was analysed using a standard PCR protocol. Polymorphisms were detected in the Opaque-2 locus among all the maize lines when using the SSR primers, phi057 and phi112, while no polymorphism was detected with primer umc1066. To facilitate background selection in the heterozygous BC2 progeny, key phenotypic characters of the recurrent parent (136R) were used as additional markers. The SSR marker phi057 was co-dominant while phi112 was dominant. The polymorphic SSR markers correctly predicted the expression of tryptophan in kernels of all QPM inbreds and five of the six non-QPM inbred lines. However, one non-QPM inbred line (PED49B) had a tryptophan content that is characteristic of the o2 mutation (0.090), suggesting that another genetic system may be responsible for the expression of tryptophan in this maize line. Phi057 was employed to monitor the introgression of the o2 allele from CML176 to 136R. Of the 200 BC1F1 (136R/CML176/136R) plants genotyped, 104 were found to be heterozygous, producing products corresponding to both alleles (o2 and O2), while 96 produced a single band corresponding to the homozygous dominant (O2O2) condition. The ratio of the two groups in the backcross (BC) population was consistent with proportion 1 :1 accorded by the Chi-square rest (X2=0.16<X2 0.05=3.84) for a single gene in a BC population. Therefore, under the conditions of PCR used, SSR markers phi057 and phi112 will constitute the framework for marker assisted introgression of the o2 trait into suitable maize genotypes in Uganda.
publishDate 2005
dc.date.none.fl_str_mv 2005
2013-06-30T05:24:27Z
2013-06-30T05:24:27Z
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10883/3028
url http://hdl.handle.net/10883/3028
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
dc.publisher.none.fl_str_mv African Crop Science Society
http://www.bioline.org.br/abstract?id=cs05022&lang=en
publisher.none.fl_str_mv African Crop Science Society
http://www.bioline.org.br/abstract?id=cs05022&lang=en
dc.source.none.fl_str_mv 4
13
African Crop Science Journal
reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
instname:Centro Internacional de Mejoramiento de Maíz y Trigo
instacron:CIMMYT
instname_str Centro Internacional de Mejoramiento de Maíz y Trigo
instacron_str CIMMYT
institution CIMMYT
reponame_str Repositorio Institucional de Publicaciones Multimedia del CIMMYT
collection Repositorio Institucional de Publicaciones Multimedia del CIMMYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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