Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles

The antiviral product cidofovir and a diet supplemented with Spirulina platensis were tested for their efficacy to prevent or delay/reduce mortality due to white spot syndrome virus (WSSV) infection in specific pathogen free (SPF) shrimp Litopenaeus vannamei juveniles. Cidofovir was injected intramu...

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Autores: Rahman, M. M., Escobedo-Bonilla, César Marcial, Wille, M., Alday-Sanz, V, Audoorn, L., Neyts, J., Pensaert, M B, Sorgeloos, P., Nauwynck, H J
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:México
Institución:Instituto Politécnico Nacional
Repositorio:Repositorio Digital del IPN
OAI Identifier:oai:www.repositoriodigital.ipn.mx:123456789/16629
Acceso en línea:http://www.repositoriodigital.ipn.mx/handle/123456789/16629
Access Level:acceso abierto
Palabra clave:Litopenaeus vannamei; SPF; WSSV; Antiviral; Cidofovir; Spirulina platensis
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spelling Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juvenilesRahman, M. M.Escobedo-Bonilla, César MarcialWille, M.Alday-Sanz, VAudoorn, L.Neyts, J.Pensaert, M BSorgeloos, P.Nauwynck, H JLitopenaeus vannamei; SPF; WSSV; Antiviral; Cidofovir; Spirulina platensisThe antiviral product cidofovir and a diet supplemented with Spirulina platensis were tested for their efficacy to prevent or delay/reduce mortality due to white spot syndrome virus (WSSV) infection in specific pathogen free (SPF) shrimp Litopenaeus vannamei juveniles. Cidofovir was injected intramuscularly at 200 mg/kg shrimp mean body weight (MBW) at the moment of WSSV challenge. Spirulina was supplemented in the shrimp diet at 25% w/w and shrimp were fed for 4 days at 5% of the MBW per day before WSSV challenge. Shrimp were inoculated orally with WSSV at a dose of 30 SID50 (SID50 = shrimp infectious dose with 50% endpoint) and clinical signs and mortality were followed for 120 h post inoculation (hpi). WSSV infection status was determined by indirect immunoflourescence (IIF) in dead and survivor shrimp at the end of the trial. In two experiments, mortality was delayed approximately for 24 h by cidofovir treatment. The 100% mortality level was reached at 96–108 hpi in mock treated shrimp, whereas in cidofovir treated shrimp, 80–90% mortality was reached at the end of experiment (120 hpi). Significant differences (p < 0.05) in the median lethal time (LT50) of cidofovir-treated shrimp and mock-treated shrimp were found by probit analysis. A Spirulina supplemented diet delayed the onset of clinical signs for 12 h but had no effect on the cumulative mortality at the end of the experiment. This study opens perspectives for antiviral drugs to treat shrimp infected with WSSV.Instituto Politécnico Nacional CIIDIR-SinaloaInterdisciplinariapdf2013-08-07T16:24:02Z2013-08-07T16:24:02Z2013-08-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://www.repositoriodigital.ipn.mx/handle/123456789/16629reponame:Repositorio Digital del IPNinstname:Instituto Politécnico Nacionalinstacron:IPNen_USinfo:eu-repo/semantics/openAccessoai:www.repositoriodigital.ipn.mx:123456789/166292026-02-18T16:45:26Z
dc.title.none.fl_str_mv Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
title Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
spellingShingle Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
Rahman, M. M.
Litopenaeus vannamei; SPF; WSSV; Antiviral; Cidofovir; Spirulina platensis
title_short Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
title_full Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
title_fullStr Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
title_full_unstemmed Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
title_sort Clinical effect of cidofovir and a diet supplemented with Spirulina platensis in white spot syndrome virus (WSSV) infected specific pathogen-free Litopenaeus vannamei juveniles
dc.creator.none.fl_str_mv Rahman, M. M.
Escobedo-Bonilla, César Marcial
Wille, M.
Alday-Sanz, V
Audoorn, L.
Neyts, J.
Pensaert, M B
Sorgeloos, P.
Nauwynck, H J
author Rahman, M. M.
author_facet Rahman, M. M.
Escobedo-Bonilla, César Marcial
Wille, M.
Alday-Sanz, V
Audoorn, L.
Neyts, J.
Pensaert, M B
Sorgeloos, P.
Nauwynck, H J
author_role author
author2 Escobedo-Bonilla, César Marcial
Wille, M.
Alday-Sanz, V
Audoorn, L.
Neyts, J.
Pensaert, M B
Sorgeloos, P.
Nauwynck, H J
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Litopenaeus vannamei; SPF; WSSV; Antiviral; Cidofovir; Spirulina platensis
topic Litopenaeus vannamei; SPF; WSSV; Antiviral; Cidofovir; Spirulina platensis
description The antiviral product cidofovir and a diet supplemented with Spirulina platensis were tested for their efficacy to prevent or delay/reduce mortality due to white spot syndrome virus (WSSV) infection in specific pathogen free (SPF) shrimp Litopenaeus vannamei juveniles. Cidofovir was injected intramuscularly at 200 mg/kg shrimp mean body weight (MBW) at the moment of WSSV challenge. Spirulina was supplemented in the shrimp diet at 25% w/w and shrimp were fed for 4 days at 5% of the MBW per day before WSSV challenge. Shrimp were inoculated orally with WSSV at a dose of 30 SID50 (SID50 = shrimp infectious dose with 50% endpoint) and clinical signs and mortality were followed for 120 h post inoculation (hpi). WSSV infection status was determined by indirect immunoflourescence (IIF) in dead and survivor shrimp at the end of the trial. In two experiments, mortality was delayed approximately for 24 h by cidofovir treatment. The 100% mortality level was reached at 96–108 hpi in mock treated shrimp, whereas in cidofovir treated shrimp, 80–90% mortality was reached at the end of experiment (120 hpi). Significant differences (p < 0.05) in the median lethal time (LT50) of cidofovir-treated shrimp and mock-treated shrimp were found by probit analysis. A Spirulina supplemented diet delayed the onset of clinical signs for 12 h but had no effect on the cumulative mortality at the end of the experiment. This study opens perspectives for antiviral drugs to treat shrimp infected with WSSV.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-07T16:24:02Z
2013-08-07T16:24:02Z
2013-08-07
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.repositoriodigital.ipn.mx/handle/123456789/16629
url http://www.repositoriodigital.ipn.mx/handle/123456789/16629
dc.language.none.fl_str_mv en_US
language_invalid_str_mv en_US
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositorio Digital del IPN
instname:Instituto Politécnico Nacional
instacron:IPN
instname_str Instituto Politécnico Nacional
instacron_str IPN
institution IPN
reponame_str Repositorio Digital del IPN
collection Repositorio Digital del IPN
repository.name.fl_str_mv
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