Comprehending the evolution of gene editing platforms for crop trait improvement

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system was initially discovered as an underlying mechanism for conferring adaptive immunity to bacteria and archaea against viruses. Over the past decade, this has been repurposed as a genome-editing tool. Num...

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Autores: Dhakate, P., Sehgal, D., Vaishnavi, S., Chandra, A., Singh, A., Raina, S.N., Vijay Rani Rajpal
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:México
Institución:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/22298
Acceso en línea:https://hdl.handle.net/10883/22298
Access Level:acceso abierto
Palabra clave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
CRISPR/Cas9
Base Editing
Prime Editing
Epigenome Editing
CRISPR
ABIOTIC STRESS
ARABIDOPSIS
CROP IMPROVEMENT
DNA
ELECTROPORATION
GENE EDITING
RICE
WHEAT
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spelling Comprehending the evolution of gene editing platforms for crop trait improvementDhakate, P.Sehgal, D.Vaishnavi, S.Chandra, A.Singh, A.Raina, S.N.Vijay Rani RajpalAGRICULTURAL SCIENCES AND BIOTECHNOLOGYCRISPR/Cas9Base EditingPrime EditingEpigenome EditingCRISPRABIOTIC STRESSARABIDOPSISCROP IMPROVEMENTDNAELECTROPORATIONGENE EDITINGRICEWHEATCRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system was initially discovered as an underlying mechanism for conferring adaptive immunity to bacteria and archaea against viruses. Over the past decade, this has been repurposed as a genome-editing tool. Numerous gene editing-based crop improvement technologies involving CRISPR/Cas platforms individually or in combination with next-generation sequencing methods have been developed that have revolutionized plant genome-editing methodologies. Initially, CRISPR/Cas nucleases replaced the earlier used sequence-specific nucleases (SSNs), such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), to address the problem of associated off-targets. The adaptation of this platform led to the development of concepts such as epigenome editing, base editing, and prime editing. Epigenome editing employed epi-effectors to manipulate chromatin structure, while base editing uses base editors to engineer precise changes for trait improvement. Newer technologies such as prime editing have now been developed as a “search-and-replace” tool to engineer all possible single-base changes. Owing to the availability of these, the field of genome editing has evolved rapidly to develop crop plants with improved traits. In this review, we present the evolution of the CRISPR/Cas system into new-age methods of genome engineering across various plant species and the impact they have had on tweaking plant genomes and associated outcomes on crop improvement initiatives.Frontiers Media2022-12-07T20:30:17Z2022-12-07T20:30:17Z2022Published Versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10883/2229810.3389/fgene.2022.876987131664-8021Frontiers in Genetics876987reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishNutrition, health & food securityAccelerated BreedingGenetic InnovationCGIAR Trust Fundhttps://hdl.handle.net/10568/129039SwitzerlandCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purposeOpen Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/222982024-10-11T19:55:41Z
dc.title.none.fl_str_mv Comprehending the evolution of gene editing platforms for crop trait improvement
title Comprehending the evolution of gene editing platforms for crop trait improvement
spellingShingle Comprehending the evolution of gene editing platforms for crop trait improvement
Dhakate, P.
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
CRISPR/Cas9
Base Editing
Prime Editing
Epigenome Editing
CRISPR
ABIOTIC STRESS
ARABIDOPSIS
CROP IMPROVEMENT
DNA
ELECTROPORATION
GENE EDITING
RICE
WHEAT
title_short Comprehending the evolution of gene editing platforms for crop trait improvement
title_full Comprehending the evolution of gene editing platforms for crop trait improvement
title_fullStr Comprehending the evolution of gene editing platforms for crop trait improvement
title_full_unstemmed Comprehending the evolution of gene editing platforms for crop trait improvement
title_sort Comprehending the evolution of gene editing platforms for crop trait improvement
dc.creator.none.fl_str_mv Dhakate, P.
Sehgal, D.
Vaishnavi, S.
Chandra, A.
Singh, A.
Raina, S.N.
Vijay Rani Rajpal
author Dhakate, P.
author_facet Dhakate, P.
Sehgal, D.
Vaishnavi, S.
Chandra, A.
Singh, A.
Raina, S.N.
Vijay Rani Rajpal
author_role author
author2 Sehgal, D.
Vaishnavi, S.
Chandra, A.
Singh, A.
Raina, S.N.
Vijay Rani Rajpal
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
CRISPR/Cas9
Base Editing
Prime Editing
Epigenome Editing
CRISPR
ABIOTIC STRESS
ARABIDOPSIS
CROP IMPROVEMENT
DNA
ELECTROPORATION
GENE EDITING
RICE
WHEAT
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
CRISPR/Cas9
Base Editing
Prime Editing
Epigenome Editing
CRISPR
ABIOTIC STRESS
ARABIDOPSIS
CROP IMPROVEMENT
DNA
ELECTROPORATION
GENE EDITING
RICE
WHEAT
description CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system was initially discovered as an underlying mechanism for conferring adaptive immunity to bacteria and archaea against viruses. Over the past decade, this has been repurposed as a genome-editing tool. Numerous gene editing-based crop improvement technologies involving CRISPR/Cas platforms individually or in combination with next-generation sequencing methods have been developed that have revolutionized plant genome-editing methodologies. Initially, CRISPR/Cas nucleases replaced the earlier used sequence-specific nucleases (SSNs), such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), to address the problem of associated off-targets. The adaptation of this platform led to the development of concepts such as epigenome editing, base editing, and prime editing. Epigenome editing employed epi-effectors to manipulate chromatin structure, while base editing uses base editors to engineer precise changes for trait improvement. Newer technologies such as prime editing have now been developed as a “search-and-replace” tool to engineer all possible single-base changes. Owing to the availability of these, the field of genome editing has evolved rapidly to develop crop plants with improved traits. In this review, we present the evolution of the CRISPR/Cas system into new-age methods of genome engineering across various plant species and the impact they have had on tweaking plant genomes and associated outcomes on crop improvement initiatives.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-07T20:30:17Z
2022-12-07T20:30:17Z
2022
dc.type.none.fl_str_mv Published Version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10883/22298
10.3389/fgene.2022.876987
url https://hdl.handle.net/10883/22298
identifier_str_mv 10.3389/fgene.2022.876987
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.relation.none.fl_str_mv Nutrition, health & food security
Accelerated Breeding
Genetic Innovation
CGIAR Trust Fund
https://hdl.handle.net/10568/129039
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Switzerland
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv 13
1664-8021
Frontiers in Genetics
876987
reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
instname:Centro Internacional de Mejoramiento de Maíz y Trigo
instacron:CIMMYT
instname_str Centro Internacional de Mejoramiento de Maíz y Trigo
instacron_str CIMMYT
institution CIMMYT
reponame_str Repositorio Institucional de Publicaciones Multimedia del CIMMYT
collection Repositorio Institucional de Publicaciones Multimedia del CIMMYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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