Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps

"Soluble arsenic (As) in acidic feed solution may inhibit the copper (Cu) bioleaching process within mine heaps. To clarify the effect of soluble arsenic on the live biomass and bioxidative activity in heaps, toxicological assays were performed using a synthetic feed solution given by a mine co...

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Detalles Bibliográficos
Autores: J SERGIO CASAS FLORES, ELIDA YAZMIN GOMEZ RODRIGUEZ, JESSICA VIRIDIANA GARCIA MEZA
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:México
Institución:Instituto Potosino de Investigación Científica y Tecnológica
Repositorio:Repositorio Institucional del IPICYT
Idioma:inglés
OAI Identifier:oai:ipicyt.repositorioinstitucional.mx:1010/1326
Acceso en línea:http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1326
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autor/Mine heaps
info:eu-repo/classification/Autor/Bacterial diversity
info:eu-repo/classification/Autor/Extremophiles
info:eu-repo/classification/Autor/Arsenic resistance system
info:eu-repo/classification/cti/2
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oai_identifier_str oai:ipicyt.repositorioinstitucional.mx:1010/1326
network_acronym_str MX
network_name_str México
repository_id_str
spelling Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heapsJ SERGIO CASAS FLORESELIDA YAZMIN GOMEZ RODRIGUEZJESSICA VIRIDIANA GARCIA MEZAinfo:eu-repo/classification/Autor/Mine heapsinfo:eu-repo/classification/Autor/Bacterial diversityinfo:eu-repo/classification/Autor/Extremophilesinfo:eu-repo/classification/Autor/Arsenic resistance systeminfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/2"Soluble arsenic (As) in acidic feed solution may inhibit the copper (Cu) bioleaching process within mine heaps. To clarify the effect of soluble arsenic on the live biomass and bioxidative activity in heaps, toxicological assays were performed using a synthetic feed solution given by a mine company. The microorganisms had previously been isolated from two heap samples at up to 66 m depth, and cultured using specific media for chemolithotrophic acidophiles (pH 1-2) and moderate thermophiles (48 degrees C), for arsenic tolerance assay. The four media with the highest biomass were selected to assay As-resistance; one culture (Q63h) was chosen to assay biooxidative activity, using a heap sample that contained chalcopyrite and covellite. We found that 0.5 g/L of As does not affect living biomass or biooxidative activity on Cu sulfides, but it dissolves Cu, while As precipitates as arsenic acid (H3AsO4 center dot 1/2H(2)O). The arsenic tolerant community, as identified by 16S rDNA gene sequence analysis, was composed of three main metabolic groups: chemolithotrophs (Leptospirillum, Sulfobacillus); chemolithoheterotrophs and organoheterotrophs as Acidovorax temperans, Pseudomonas alcaligenes, P. mendocina and Sphingomonas spp. Leptospirillum spp. and S. thermosulfidooxidans were the dominant taxa in the Q63-66 cultures from the deepest sample of the oldest, highest-temperature heap. The results indicated arsenic resistance in the microbial community, therefore specific primers were used to amplify ars (arsenic resistance system), aio (arsenite oxidase), or arr (arsenate respiratory reduction) genes from total sample DNA. Presence of arsB genes in S. thermosulfidooxidans in the Q63-66 cultures permits H3AsO4-As(V) detoxification and strengthens the community's response to As."BioMed Central LTD2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1326reponame:Repositorio Institucional del IPICYTinstname:Instituto Potosino de Investigación Científica y Tecnológicainstacron:IPICYTenginfo:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1186/s13568-015-0132-5citation:Casas-Flores, S., Gómez-Rodríguez, E. Y., & García-Meza, J. V. (2015). Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps. AMB Express, 5, 54. http://doi.org/10.1186/s13568-015-0132-5info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:ipicyt.repositorioinstitucional.mx:1010/13262024-08-28T03:17:38Z
dc.title.none.fl_str_mv Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
title Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
spellingShingle Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
J SERGIO CASAS FLORES
info:eu-repo/classification/Autor/Mine heaps
info:eu-repo/classification/Autor/Bacterial diversity
info:eu-repo/classification/Autor/Extremophiles
info:eu-repo/classification/Autor/Arsenic resistance system
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
title_short Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
title_full Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
title_fullStr Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
title_full_unstemmed Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
title_sort Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps
dc.creator.none.fl_str_mv J SERGIO CASAS FLORES
ELIDA YAZMIN GOMEZ RODRIGUEZ
JESSICA VIRIDIANA GARCIA MEZA
author J SERGIO CASAS FLORES
author_facet J SERGIO CASAS FLORES
ELIDA YAZMIN GOMEZ RODRIGUEZ
JESSICA VIRIDIANA GARCIA MEZA
author_role author
author2 ELIDA YAZMIN GOMEZ RODRIGUEZ
JESSICA VIRIDIANA GARCIA MEZA
author2_role author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/Autor/Mine heaps
info:eu-repo/classification/Autor/Bacterial diversity
info:eu-repo/classification/Autor/Extremophiles
info:eu-repo/classification/Autor/Arsenic resistance system
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
topic info:eu-repo/classification/Autor/Mine heaps
info:eu-repo/classification/Autor/Bacterial diversity
info:eu-repo/classification/Autor/Extremophiles
info:eu-repo/classification/Autor/Arsenic resistance system
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
description "Soluble arsenic (As) in acidic feed solution may inhibit the copper (Cu) bioleaching process within mine heaps. To clarify the effect of soluble arsenic on the live biomass and bioxidative activity in heaps, toxicological assays were performed using a synthetic feed solution given by a mine company. The microorganisms had previously been isolated from two heap samples at up to 66 m depth, and cultured using specific media for chemolithotrophic acidophiles (pH 1-2) and moderate thermophiles (48 degrees C), for arsenic tolerance assay. The four media with the highest biomass were selected to assay As-resistance; one culture (Q63h) was chosen to assay biooxidative activity, using a heap sample that contained chalcopyrite and covellite. We found that 0.5 g/L of As does not affect living biomass or biooxidative activity on Cu sulfides, but it dissolves Cu, while As precipitates as arsenic acid (H3AsO4 center dot 1/2H(2)O). The arsenic tolerant community, as identified by 16S rDNA gene sequence analysis, was composed of three main metabolic groups: chemolithotrophs (Leptospirillum, Sulfobacillus); chemolithoheterotrophs and organoheterotrophs as Acidovorax temperans, Pseudomonas alcaligenes, P. mendocina and Sphingomonas spp. Leptospirillum spp. and S. thermosulfidooxidans were the dominant taxa in the Q63-66 cultures from the deepest sample of the oldest, highest-temperature heap. The results indicated arsenic resistance in the microbial community, therefore specific primers were used to amplify ars (arsenic resistance system), aio (arsenite oxidase), or arr (arsenate respiratory reduction) genes from total sample DNA. Presence of arsB genes in S. thermosulfidooxidans in the Q63-66 cultures permits H3AsO4-As(V) detoxification and strengthens the community's response to As."
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1326
url http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1326
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1186/s13568-015-0132-5
citation:Casas-Flores, S., Gómez-Rodríguez, E. Y., & García-Meza, J. V. (2015). Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps. AMB Express, 5, 54. http://doi.org/10.1186/s13568-015-0132-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central LTD
publisher.none.fl_str_mv BioMed Central LTD
dc.source.none.fl_str_mv reponame:Repositorio Institucional del IPICYT
instname:Instituto Potosino de Investigación Científica y Tecnológica
instacron:IPICYT
instname_str Instituto Potosino de Investigación Científica y Tecnológica
instacron_str IPICYT
institution IPICYT
reponame_str Repositorio Institucional del IPICYT
collection Repositorio Institucional del IPICYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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