Identification of in vivo induced maternal haploids in maize using seedling traits

In vivo haploid induction in high frequency followed by efficient identification of haploids are important components of deriving completely homozygous doubled haploid (DH) lines in maize. Several genetic marker systems were proposed and/or used for identification of in vivo maternal haploids in mai...

Descripción completa

Detalles Bibliográficos
Autores: Chaikam, V., Lopez, L.A., Martinez, L., Burgueño, J., Prasanna, B.M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:México
Institución:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/19013
Acceso en línea:http://hdl.handle.net/10883/19013
Access Level:acceso abierto
Palabra clave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
MAIZE
HAPLOIDY
SEEDLINGS
id MX_57f97735cf5a252ff2ba6f6d033d1275
oai_identifier_str oai:repository.cimmyt.org:10883/19013
network_acronym_str MX
network_name_str México
repository_id_str
spelling Identification of in vivo induced maternal haploids in maize using seedling traitsChaikam, V.Lopez, L.A.Martinez, L.Burgueño, J.Prasanna, B.M.AGRICULTURAL SCIENCES AND BIOTECHNOLOGYMAIZEHAPLOIDYSEEDLINGSIn vivo haploid induction in high frequency followed by efficient identification of haploids are important components of deriving completely homozygous doubled haploid (DH) lines in maize. Several genetic marker systems were proposed and/or used for identification of in vivo maternal haploids in maize, such as R1-nj (Navajo), high oil, red root and transgenic markers. In this study, we propose a new method of haploid/diploid identification based on natural differences in seedling traits of haploids and diploids, which can be used in any induction cross independently of the genetic marker systems. Using confirmed haploids and diploids from five different populations, the study established that haploid and diploid seedlings exhibit significant differences for seedling traits, particularly radicle length (RL), coleoptile length (CL), and number of lateral seminal roots (NLSR). In six populations that exhibited complete inhibition of the commonly used R1-nj (Navajo) marker, we could effectively differentiate haploids from diploids by visual inspection of the seedling traits. In the haploid seed fraction identified based on R1-nj marker in ten populations, false positives were reduced several-fold by early identification of haploids at seedling stage using the seedling traits. We propose that seedling traits may be integrated at the haploid identification stage, especially in populations that are not amenable to use of genetic markers, and for improving the efficiency of DH line production by reducing the false positives.Springer2017-09-26T15:06:46Z2017-09-26T15:06:46Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePDFapplication/pdfhttp://hdl.handle.net/10883/1901310.1007/s10681-017-1968-38213Euphytica177reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishhttps://static-content.springer.com/esm/art%3A10.1007%2Fs10681-017-1968-3/MediaObjects/10681_2017_1968_MOESM1_ESM.pdfNetherlandsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.Open Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/190132024-10-11T19:55:58Z
dc.title.none.fl_str_mv Identification of in vivo induced maternal haploids in maize using seedling traits
title Identification of in vivo induced maternal haploids in maize using seedling traits
spellingShingle Identification of in vivo induced maternal haploids in maize using seedling traits
Chaikam, V.
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
MAIZE
HAPLOIDY
SEEDLINGS
title_short Identification of in vivo induced maternal haploids in maize using seedling traits
title_full Identification of in vivo induced maternal haploids in maize using seedling traits
title_fullStr Identification of in vivo induced maternal haploids in maize using seedling traits
title_full_unstemmed Identification of in vivo induced maternal haploids in maize using seedling traits
title_sort Identification of in vivo induced maternal haploids in maize using seedling traits
dc.creator.none.fl_str_mv Chaikam, V.
Lopez, L.A.
Martinez, L.
Burgueño, J.
Prasanna, B.M.
author Chaikam, V.
author_facet Chaikam, V.
Lopez, L.A.
Martinez, L.
Burgueño, J.
Prasanna, B.M.
author_role author
author2 Lopez, L.A.
Martinez, L.
Burgueño, J.
Prasanna, B.M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
MAIZE
HAPLOIDY
SEEDLINGS
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
MAIZE
HAPLOIDY
SEEDLINGS
description In vivo haploid induction in high frequency followed by efficient identification of haploids are important components of deriving completely homozygous doubled haploid (DH) lines in maize. Several genetic marker systems were proposed and/or used for identification of in vivo maternal haploids in maize, such as R1-nj (Navajo), high oil, red root and transgenic markers. In this study, we propose a new method of haploid/diploid identification based on natural differences in seedling traits of haploids and diploids, which can be used in any induction cross independently of the genetic marker systems. Using confirmed haploids and diploids from five different populations, the study established that haploid and diploid seedlings exhibit significant differences for seedling traits, particularly radicle length (RL), coleoptile length (CL), and number of lateral seminal roots (NLSR). In six populations that exhibited complete inhibition of the commonly used R1-nj (Navajo) marker, we could effectively differentiate haploids from diploids by visual inspection of the seedling traits. In the haploid seed fraction identified based on R1-nj marker in ten populations, false positives were reduced several-fold by early identification of haploids at seedling stage using the seedling traits. We propose that seedling traits may be integrated at the haploid identification stage, especially in populations that are not amenable to use of genetic markers, and for improving the efficiency of DH line production by reducing the false positives.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-26T15:06:46Z
2017-09-26T15:06:46Z
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10883/19013
10.1007/s10681-017-1968-3
url http://hdl.handle.net/10883/19013
identifier_str_mv 10.1007/s10681-017-1968-3
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.relation.none.fl_str_mv https://static-content.springer.com/esm/art%3A10.1007%2Fs10681-017-1968-3/MediaObjects/10681_2017_1968_MOESM1_ESM.pdf
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
dc.coverage.none.fl_str_mv Netherlands
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv 8
213
Euphytica
177
reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
instname:Centro Internacional de Mejoramiento de Maíz y Trigo
instacron:CIMMYT
instname_str Centro Internacional de Mejoramiento de Maíz y Trigo
instacron_str CIMMYT
institution CIMMYT
reponame_str Repositorio Institucional de Publicaciones Multimedia del CIMMYT
collection Repositorio Institucional de Publicaciones Multimedia del CIMMYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1858175336477360128
score 15.808905

Similares en LA Referencia