ANALISIS DE DIVERSIDAD GENETICA DE 64 VARIEDADES DE JAMAICA (HIBISCUS SABDARIFFA L.) MEDIANTE MARCADORES MOLECULARES
The Jamaican crop (Hibiscus sabdariffa L.) is originally from Africa, belongs to the Malvaceae family. In our country, it is cultivated in 15 states of the Republic. The genetic diversity present in the varieties currently used is given mainly by natural crosses and selection, however, there is a ga...
| Autores: | , |
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| Formato: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Recursos: | Universidad Autónoma de Nayarit |
| Repositorio: | Repositorio Institucional Aramara de la UAN |
| Idioma: | español |
| OAI Identifier: | oai:dspace.uan.mx:123456789/1369 |
| Acesso em linha: | http://dspace.uan.mx:8080/jspui/handle/123456789/1369 |
| Access Level: | acceso abierto |
| Palavra-chave: | cultivo de Jamaica (Hibiscus sabdariffa L.) diversidad genética fenograma cultivation of Jamaica (Hibiscus sabdariffa L.) genetic diversity phenogram CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA [6] |
| Resumo: | The Jamaican crop (Hibiscus sabdariffa L.) is originally from Africa, belongs to the Malvaceae family. In our country, it is cultivated in 15 states of the Republic. The genetic diversity present in the varieties currently used is given mainly by natural crosses and selection, however, there is a gap in varietal identification, studies on diversity and genetic structure of them. Although there are some international studies, the number of samples used has been limited or have focused on other species of Hibiscus. For this reason, it is necessary to estimate the genetic diversity of Jamaican varieties at both morphological and molecular levels. The objective of the present work was to estimate, through the use of molecular markers, the levels of genetic diversity present in 64 accessions from Jamaica (Hibiscus sabdariffa L.). 64 accessions originating from Nayarit, Guerrero, Colima and Puebla were included. Genomic DNA was obtained from lyophilized foliar tissue using the modified CTAB method. For the molecular analysis the combinations of primers derived from Cytochrome P450, CYP2B6F/CYP1A1 R, CYP1A1F/CYP2B6R, CYP1A1F/CYP2C19R, CYP2C19F/CYP2C19R, CYP2B6F/CYP2B6R and CYP2B6F/CYP2C19R were used, In addition, five combinations of AFLPs were amplified which were highly informative Eco RI + AAG/Mse I + CAC, Eco RI + ACG/Mse I + CAAG, Eco RI + AAG/Mse 1+ CAG, Eco RI+ ACG/Mse I +CAGC and Eco RI + AAG/Mse I + CGC. The amplified fragments were displayed in polyacrylamide gels (8%). The basic parameters of genetic diversity and the analysis of main coordinates (PCoA) were performed with GenAlEx 6.5 software. The phonogram was constructed with the genetic distance matrix using the Neighbor-Joining (N-J) methodology, with the Mega 5.2 software. The results of the combinations of CYP450 indicated 82.65% polymorphism and 0.403 of total expected heterozygosity, the latter being considered as moderate to high, compared with other results previously reported (0.305, using AFLP-s markers). Through the PCoA 74.76% of the genetic variation was explained, while in the N-J phenogram the Jamaican samples were separated into three main groups and each one with two subgroups and subdivisions. This last analysis clearly separated the progenitors that originated the Nayarit materials, placing them at the opposite ends of the phonogram, while the rest of the materials showed no trend in clustering. In the case of AFLPs, 86.57% polymorphism was observed, with an average of 32.4 polymorphic loci per combination of markers. The genetic diversity of (Nei) was 0.310, values similar to those reported in other studies (0.305, also with AFLPs markers). It was established that there are no duplicate genotypes in the collection, however, there is a high degree of kinship in the materials according to the dispersion of the phenotype. |
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