In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)

Psittacanthus calyculatus is a hemiparasitic plant that affects various plant species of great importance to the ecosystem. Contains various compounds withantioxidant and antimicrobial activity. There are various reports on its antibacterial capacity;however, there is no information available on its...

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Autores: Xoca-Orozco, Luis Angel, Cortez-Fonseca, Karina, Luna-López, Carlos, Hernández-Mendoza, Gustavo, Flores-Sierra, José de Jesús, Chacón-López, Martina Alejandra, Aguilera-Aguirre, Selene
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:México
Recursos:UNIVERSIDAD AUTÓNOMA DE TABASCO
Repositorio:Ecosistemas y Recursos Agropecuarios
Idioma:español
OAI Identifier:oai:era.ujat.mx:article/3431
Acesso em linha:https://era.ujat.mx/index.php/rera/article/view/3431
Access Level:acceso abierto
Palavra-chave:Colletotrichum gloeosporioides
Curvularia
Fusarium
fungistatic
antifungal
fungistático
antifúngico
id MX_4b0307ff64db097f1a2aba7ea6ca19c6
oai_identifier_str oai:era.ujat.mx:article/3431
network_acronym_str MX
network_name_str México
repository_id_str
dc.title.none.fl_str_mv In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
Inhibición in vitro de hongos fitopatógenos utilizando extractos de muérdago mexicano (Psittacanthus calyculatus)
title In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
spellingShingle In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
Xoca-Orozco, Luis Angel
Colletotrichum gloeosporioides
Curvularia
Fusarium
fungistatic
antifungal
Colletotrichum gloeosporioides
Curvularia
Fusarium
fungistático
antifúngico
title_short In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
title_full In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
title_fullStr In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
title_full_unstemmed In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
title_sort In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)
dc.creator.none.fl_str_mv Xoca-Orozco, Luis Angel
Cortez-Fonseca, Karina
Luna-López, Carlos
Hernández-Mendoza, Gustavo
Flores-Sierra, José de Jesús
Chacón-López, Martina Alejandra
Aguilera-Aguirre, Selene
author Xoca-Orozco, Luis Angel
author_facet Xoca-Orozco, Luis Angel
Cortez-Fonseca, Karina
Luna-López, Carlos
Hernández-Mendoza, Gustavo
Flores-Sierra, José de Jesús
Chacón-López, Martina Alejandra
Aguilera-Aguirre, Selene
author_role author
author2 Cortez-Fonseca, Karina
Luna-López, Carlos
Hernández-Mendoza, Gustavo
Flores-Sierra, José de Jesús
Chacón-López, Martina Alejandra
Aguilera-Aguirre, Selene
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Colletotrichum gloeosporioides
Curvularia
Fusarium
fungistatic
antifungal
Colletotrichum gloeosporioides
Curvularia
Fusarium
fungistático
antifúngico
topic Colletotrichum gloeosporioides
Curvularia
Fusarium
fungistatic
antifungal
Colletotrichum gloeosporioides
Curvularia
Fusarium
fungistático
antifúngico
description Psittacanthus calyculatus is a hemiparasitic plant that affects various plant species of great importance to the ecosystem. Contains various compounds withantioxidant and antimicrobial activity. There are various reports on its antibacterial capacity;however, there is no information available on its antifungal activity. That is why the aim of this work was to show the antifungal activity of mistletoe extracts against the inhibition of Colletotrichum gloeosporioides, Fusarium sp. and Curvularia sp. For this, both mistletoe leaves and flowers obtained from two hosts were used: Prosopis laevigata and Eysenhardtia polystachya. The samples were washed, dehydrated and ground. Subsequently, a solid-liquid extraction was carried out using methanol-acetone-water. The content of phenols and flavonoids was quantified, as well as the phytochemical profile qualitatively. The evaluation ofthe antifungal activity was carried out with the plate dilution technique with PDA medium. Theparameters of inhibition of mycelial growth, sporulation, germination was evaluated and the fungicidal or fungistatic effect was determined. The results showed an inhibition greater than 70% using both leaf and flower extracts, with Curvularia sp. being the most susceptible with 98% inhibition. There was also a reduction in sporulation and germination of the evaluatedphytopathogens. It was determined that the inhibition corresponds to a fungistatic effect. The results reveal a high in vitro inhibition capacity of mistletoe extracts against phytopathogens of great importance for agriculture.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://era.ujat.mx/index.php/rera/article/view/3431
10.19136/era.a9n3.3431
url https://era.ujat.mx/index.php/rera/article/view/3431
identifier_str_mv 10.19136/era.a9n3.3431
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://era.ujat.mx/index.php/rera/article/view/3431/1622
dc.rights.none.fl_str_mv Derechos de autor 2022 Ecosistemas y Recursos Agropecuarios
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2022 Ecosistemas y Recursos Agropecuarios
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Juárez Autónoma de Tabasco
publisher.none.fl_str_mv Universidad Juárez Autónoma de Tabasco
dc.source.none.fl_str_mv Ecosistemas y Recursos Agropecuarios; Vol. 9 No. 3 (2022): septiembre-diciembre 2022
Ecosistemas y Recursos Agropecuarios; Vol. 9 Núm. 3 (2022): septiembre-diciembre 2022
2007-901X
2007-9028
reponame:Ecosistemas y Recursos Agropecuarios
instname:UNIVERSIDAD AUTÓNOMA DE TABASCO
instacron:UJAT
instname_str UNIVERSIDAD AUTÓNOMA DE TABASCO
instacron_str UJAT
institution UJAT
reponame_str Ecosistemas y Recursos Agropecuarios
collection Ecosistemas y Recursos Agropecuarios
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling In vitro inhibition of phytopathogenic fungi using extracts of Mexican mistletoe (Psittacanthus calyculatus)Inhibición in vitro de hongos fitopatógenos utilizando extractos de muérdago mexicano (Psittacanthus calyculatus)Xoca-Orozco, Luis AngelCortez-Fonseca, KarinaLuna-López, CarlosHernández-Mendoza, GustavoFlores-Sierra, José de JesúsChacón-López, Martina AlejandraAguilera-Aguirre, SeleneColletotrichum gloeosporioidesCurvulariaFusariumfungistaticantifungalColletotrichum gloeosporioidesCurvulariaFusariumfungistáticoantifúngicoPsittacanthus calyculatus is a hemiparasitic plant that affects various plant species of great importance to the ecosystem. Contains various compounds withantioxidant and antimicrobial activity. There are various reports on its antibacterial capacity;however, there is no information available on its antifungal activity. That is why the aim of this work was to show the antifungal activity of mistletoe extracts against the inhibition of Colletotrichum gloeosporioides, Fusarium sp. and Curvularia sp. For this, both mistletoe leaves and flowers obtained from two hosts were used: Prosopis laevigata and Eysenhardtia polystachya. The samples were washed, dehydrated and ground. Subsequently, a solid-liquid extraction was carried out using methanol-acetone-water. The content of phenols and flavonoids was quantified, as well as the phytochemical profile qualitatively. The evaluation ofthe antifungal activity was carried out with the plate dilution technique with PDA medium. Theparameters of inhibition of mycelial growth, sporulation, germination was evaluated and the fungicidal or fungistatic effect was determined. The results showed an inhibition greater than 70% using both leaf and flower extracts, with Curvularia sp. being the most susceptible with 98% inhibition. There was also a reduction in sporulation and germination of the evaluatedphytopathogens. It was determined that the inhibition corresponds to a fungistatic effect. The results reveal a high in vitro inhibition capacity of mistletoe extracts against phytopathogens of great importance for agriculture.Psittacanthus calyculatus es una planta hemiparásita que afecta diversas especies vegetales de gran importancia para el ecosistema. Existen diversos reportes sobre su capacidad antibacteriana debido a que contiene diversos compuestos con actividad antioxidante y antimicrobiana, sin embargo, sobre su actividad antifúngica es prácticamente nula la información disponible. Por lo que el objetivo del presente trabajo fue conocer la actividad antifúngica de extractos de muérdago ante la inhibición de Colletotrichum. gloeosporioides, Fusarium sp. y Curvularia sp. Para ello, se utilizó hoja y flor de muérdago obtenidos de dos hospederos: Prosopis laevigata y Eysenhardtia polystachya. Las muestras se lavaron, deshidrataron y molieron. Posteriormente se realizó una extracción sólido-líquido utilizando el metanol-acetona-agua. Se cuantificó el contenido de fenoles y flavonoides, además el perfil fitoquímico cualitativo. La evaluación de la actividad antifúngica se llevó a cabo con la técnica de dilución en placa con medio PDA. Se determinaron los parámetros de inhibición del crecimiento micelial, esporulación, germinación y se determinó el efecto fungicida o fungistático. Los resultados muestran inhibición mayor al 70% con extractos de hoja y flor, siendo Curvularia sp. el más susceptible con un 98% de inhibición. También hubo reducción de la esporulación y germinación de los fitopatógenos evaluados. Se determinó que la inhibición corresponde a un efecto fungistático. Los resultados revelan alta capacidadde inhibición in vitro de extractos de muérdago contra fitopatógenos de gran importancia para la agricultura.Universidad Juárez Autónoma de Tabasco2022-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://era.ujat.mx/index.php/rera/article/view/343110.19136/era.a9n3.3431Ecosistemas y Recursos Agropecuarios; Vol. 9 No. 3 (2022): septiembre-diciembre 2022Ecosistemas y Recursos Agropecuarios; Vol. 9 Núm. 3 (2022): septiembre-diciembre 20222007-901X2007-9028reponame:Ecosistemas y Recursos Agropecuariosinstname:UNIVERSIDAD AUTÓNOMA DE TABASCOinstacron:UJATspahttps://era.ujat.mx/index.php/rera/article/view/3431/1622Derechos de autor 2022 Ecosistemas y Recursos Agropecuarioshttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:era.ujat.mx:article/34312024-08-22T18:05:20Z
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