Bougainvillea spectabilis as a mediator for the biosynthesis of silver nanoparticles, evaluation of its antimicrobial and cytotoxic effect

The present study describes the biosynthesis, characterization, evaluation of the antimicrobial activity and cytotoxicity of silver nanoparticles (Ag-NPs) synthesized from the aqueous extract of the flowers and bracts of the plant Bougainvillea spectabilis. Different extract volumes and concentratio...

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Detalles Bibliográficos
Autores: Ramírez-Herrera, Angel Daniel, Barbosa-Sabanero, Gloria, Lazo-de-la-Vega-Monroy, María-Luisa, González-Domínguez, Martha-Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:México
Institución:UNIVERSIDAD DE SONORA
Repositorio:Biotecnia
Idioma:español
OAI Identifier:oai:oai.biotecnia.unison.mx:article/2257
Acceso en línea:https://biotecnia.unison.mx/index.php/biotecnia/article/view/2257
Access Level:acceso abierto
Palabra clave:Biosynthesis
nanoparticles
antimicrobial
cytotoxic
Biosíntesis
nanopartículas
antimicrobiana
citotoxicidad
Descripción
Sumario:The present study describes the biosynthesis, characterization, evaluation of the antimicrobial activity and cytotoxicity of silver nanoparticles (Ag-NPs) synthesized from the aqueous extract of the flowers and bracts of the plant Bougainvillea spectabilis. Different extract volumes and concentrations of silver nitrate were evaluated on the morphology, size, and antimicrobial activity of Ag-NPs. The formation of these nanoparticles (NPs) was monitored by ultraviolet-visible spectrophotometry (UV-Vis). Morphology and elemental analysis were performed by scanning electron microscopy coupled with X-ray energy dispersive spectrophotometry (SEM-EDS), the agglomerates of particles with dimensions ranging from 40 nm to 60 nm were observed. The presence of silver was validated with X-ray diffraction (XRD) analysis. The synthesis of Ag-NPs showed antimicrobial activity against some microorganisms isolated from diabetic foot infections, as well as control strains finding greater efficacy against Gram-negative bacteria than their Gram-positive counterparts. Ag-NPs showed cytotoxic activity in eukaryotic cells depending on their concentration, and in some cases increased cell proliferation at low concentrations. These results invite a more in-depth evaluation of the cytotoxic effect of these nanoparticles synthesized by this methodology for future application in the healthcare field.