EFFECT OF EXPLANT SOURCE ON REGENERATION AND GENETIC TRANSFORMATION EFFICIENCY IN GALIA MELON (Cucumis melo L.) MALE AND FEMALE PARENTAL LINES
The goal of this work was to investigate the capacity of cotyledon, hypocotyl and true-leaf explants of both female and male parental lines of Galia muskmelon to regenerate in vitro plants and to test their efficiency for Agrobacterium-mediated transformation. Agrobacterium tumefaciens strains (ABI...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | México |
| Institución: | Centro de Investigación y de Estudios Avanzados del IPN |
| Repositorio: | Redalyc-CINVESTAV |
| OAI Identifier: | oai:redalyc.org:30220203005 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=30220203005 |
| Access Level: | acceso abierto |
| Palabra clave: | Agrociencias plant regeneration plant transformation Agrobacterium tumefaciens |
| Sumario: | The goal of this work was to investigate the capacity of cotyledon, hypocotyl and true-leaf explants of both female and male parental lines of Galia muskmelon to regenerate in vitro plants and to test their efficiency for Agrobacterium-mediated transformation. Agrobacterium tumefaciens strains (ABI and AGL-1) containing plasmids with the reporter uidA gene encoding for ¿-glucuronidase (GUS) or the reporter gene encoding the green fluorescent protein (GFP) were used for transformation. Regeneration of shoots was achieved with all types of explants. The highest Bud Forming Capacity index (BFC) was 6.99 and GUS positive shoots and roots (10.0%) (p¿0.05) was obtained from cotyledons. Hypocotyl explants produced the highest number (13.3%) (p¿0.05) of GFP positive roots and shoots. True-leaf explants were not able to regenerate any transgenic shoots using either tested vector.Complete transgenic plants from both parental lines were regenerated in 3-4 months using cotyledons, whereas at least 6-7 months were needed when the hypocotyls were used. Flowcytometry analysis revealed that only 10% of the transgenic plants were diploid. |
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