Expresión de la Hormona Inhibidora de las Gonadotropinas (GnIH) y de la Hormona Liberadora de Gonadotropinas (GnRH) en el hipotálamo de ratas adultas estresadas
Prenatal stress (PS) in mammals is a factor involved in fetal programming of long term reproductive disorders. When PE is present during critical stages of fetal development it can cause alterations in adrenal and gonadal axes. Currently, it is unknown whether these changes are involved in the neuro...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:r781wg024 |
| Acceso en línea: | https://doi.org/10.24275/uami.r781wg024 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Mamíferos info:eu-repo/classification/LEM/Hipotálamo info:eu-repo/classification/LEM/Estrés prenatal info:eu-repo/classification/LEM/Hypothalamus info:eu-repo/classification/LEM/Mammals info:eu-repo/classification/LEM/Prenatal stress info:eu-repo/classification/LEM/Hormones, Sex, info:eu-repo/classification/LEM/Hormonas sexuales info:eu-repo/classification/cti/2 |
| Sumario: | Prenatal stress (PS) in mammals is a factor involved in fetal programming of long term reproductive disorders. When PE is present during critical stages of fetal development it can cause alterations in adrenal and gonadal axes. Currently, it is unknown whether these changes are involved in the neuroendocrine control of reproduction. Therefore, the aim of this study was to evaluate changes in the content and expression of gonadotropin-releasing hormone (GnRH), as well as gonadotropin-inhibiting hormone (GnIH) in the hypothalamus of prenatally stressed adult male and female rats, as well as their serum sex hormone levels. Pregnant females were assigned to control or stress by immersion in cold water group. The pups were weaned on day 21, sexed and separated into males and females. At the age of 3 months, animals of both sexes were sacrificed to obtain the hypothalamus and blood serum. Females were sacrificed at proestrus or diestrus stages of the estrous cycle. The hypothalamic content of GnRH and GnIH was evaluated by Western blotting and mRNA expression for these hormones was quantified by semi-quantitative RT-PCR. Sex hormones were evaluated by ELISA. A decrease in GnRH content and an increase in GnIH content was observed in the hypothalamus of PE females at both proestrus and diestrus stages. In PE males an increase in GnIH together with a decrease in GnRH was also observed. These results indicate that PE can disrupt the neuroendocrine control of reproduction in both, females and males, and these effects may cause endocrine disorders impairing reproductive function in both sexes during adulthood |
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