Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants
Ovine Enzootic Abortion (OEA) is caused by Chlamydia abortus and has recently been recognized as endemic in Mexico. Due to the impact of OEA on intensive lamb production, it is necessary to establish a sanitary control plan based on an efficient diagnosis. The loop-mediated isothermal amplification...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | México |
| Institución: | Universidad Nacional Autónoma de México |
| Repositorio: | Redalyc-UNAM |
| OAI Identifier: | oai:redalyc.org:672978747008 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=672978747008 https://www.redalyc.org/journal/6729/672978747008/ https://www.redalyc.org/journal/6729/672978747008/html/ https://www.redalyc.org/journal/6729/672978747008/672978747008.epub https://www.redalyc.org/journal/6729/672978747008/movil |
| Access Level: | acceso abierto |
| Palabra clave: | Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion |
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Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminantsCarlos Eduardo Aragón-LópezErika Gabriela Palomares-ReséndizSergio Cuevas-TellecheaJavier Rolando Reyna-GranadosBeatriz Arellano-ReynosoMiguel Angel Sánchez-CastroJosé Clemente Leyva-CoronaMarcela Ivone Morales-PablosMultidisciplinaria (Ciencias Naturales y Exactas)LAMPompA geneChlamydia abortusOvine Enzootic AbortionOvine Enzootic Abortion (OEA) is caused by Chlamydia abortus and has recently been recognized as endemic in Mexico. Due to the impact of OEA on intensive lamb production, it is necessary to establish a sanitary control plan based on an efficient diagnosis. The loop-mediated isothermal amplification technique (LAMP) is a molecular test that can specifically identify C. abortus. The aim of this study was to standardize the LAMP technique for the specific detection of C. abortus in ovine vaginal exudates. Primers were identified and modified from an electronic database. The effective conditions of the technique were determined using synthetic DNA and positive biological samples by PCR as references. The assay detection limit was 1 × 10-5 of genetic material, equivalent to 8.5 copies. The standardized LAMP test is proposed as a novel molecular tool for the rapid and specific diagnosis of C. abortus infection from ovine vaginal exudate samples in Mexico.Universidad de Sonora2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf1665-1456https://www.redalyc.org/articulo.oa?id=672978747008https://www.redalyc.org/journal/6729/672978747008/https://www.redalyc.org/journal/6729/672978747008/html/https://www.redalyc.org/journal/6729/672978747008/672978747008.epubhttps://www.redalyc.org/journal/6729/672978747008/movil10.18633/biotecnia.v26i1.2109Biotecnia (México) Vol.26reponame:Redalyc-UNAMinstname:Universidad Nacional Autónoma de Méxicoinstacron:UNAMenhttp://www.redalyc.org/revista.oa?id=6729Biotecniainfo:eu-repo/semantics/openAccessoai:redalyc.org:6729787470082025-09-03T18:11:47Z |
| dc.title.none.fl_str_mv |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| title |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| spellingShingle |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants Carlos Eduardo Aragón-López Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion |
| title_short |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| title_full |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| title_fullStr |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| title_full_unstemmed |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| title_sort |
Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| dc.creator.none.fl_str_mv |
Carlos Eduardo Aragón-López Erika Gabriela Palomares-Reséndiz Sergio Cuevas-Tellechea Javier Rolando Reyna-Granados Beatriz Arellano-Reynoso Miguel Angel Sánchez-Castro José Clemente Leyva-Corona Marcela Ivone Morales-Pablos |
| author |
Carlos Eduardo Aragón-López |
| author_facet |
Carlos Eduardo Aragón-López Erika Gabriela Palomares-Reséndiz Sergio Cuevas-Tellechea Javier Rolando Reyna-Granados Beatriz Arellano-Reynoso Miguel Angel Sánchez-Castro José Clemente Leyva-Corona Marcela Ivone Morales-Pablos |
| author_role |
author |
| author2 |
Erika Gabriela Palomares-Reséndiz Sergio Cuevas-Tellechea Javier Rolando Reyna-Granados Beatriz Arellano-Reynoso Miguel Angel Sánchez-Castro José Clemente Leyva-Corona Marcela Ivone Morales-Pablos |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion |
| topic |
Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion |
| description |
Ovine Enzootic Abortion (OEA) is caused by Chlamydia abortus and has recently been recognized as endemic in Mexico. Due to the impact of OEA on intensive lamb production, it is necessary to establish a sanitary control plan based on an efficient diagnosis. The loop-mediated isothermal amplification technique (LAMP) is a molecular test that can specifically identify C. abortus. The aim of this study was to standardize the LAMP technique for the specific detection of C. abortus in ovine vaginal exudates. Primers were identified and modified from an electronic database. The effective conditions of the technique were determined using synthetic DNA and positive biological samples by PCR as references. The assay detection limit was 1 × 10-5 of genetic material, equivalent to 8.5 copies. The standardized LAMP test is proposed as a novel molecular tool for the rapid and specific diagnosis of C. abortus infection from ovine vaginal exudate samples in Mexico. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
1665-1456 https://www.redalyc.org/articulo.oa?id=672978747008 https://www.redalyc.org/journal/6729/672978747008/ https://www.redalyc.org/journal/6729/672978747008/html/ https://www.redalyc.org/journal/6729/672978747008/672978747008.epub https://www.redalyc.org/journal/6729/672978747008/movil 10.18633/biotecnia.v26i1.2109 |
| identifier_str_mv |
1665-1456 10.18633/biotecnia.v26i1.2109 |
| url |
https://www.redalyc.org/articulo.oa?id=672978747008 https://www.redalyc.org/journal/6729/672978747008/ https://www.redalyc.org/journal/6729/672978747008/html/ https://www.redalyc.org/journal/6729/672978747008/672978747008.epub https://www.redalyc.org/journal/6729/672978747008/movil |
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en |
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en |
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http://www.redalyc.org/revista.oa?id=6729 |
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Biotecnia info:eu-repo/semantics/openAccess |
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Biotecnia |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad de Sonora |
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Universidad de Sonora |
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Biotecnia (México) Vol.26 reponame:Redalyc-UNAM instname:Universidad Nacional Autónoma de México instacron:UNAM |
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Universidad Nacional Autónoma de México |
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UNAM |
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UNAM |
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Redalyc-UNAM |
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Redalyc-UNAM |
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