Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor

"The hydrogen (H2) production efficiency in dark fermentation systems is strongly dependent on the occurrence of metabolic pathways derived from the selection of microbial species that either consume molecular H2 or outcompete hydrogenogenic bacteria for the organic substrate. In this study, th...

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Autores: Rodolfo Palomo Briones, Eric Trably, NGUYEN ESMERALDA LOPEZ LOZANO, María de Lourdes Berenice Celis García, Hugo Óscar Méndez Acosta, Nicolas BERNET, Elías Razo Flores
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2018
País:México
Institución:Instituto Potosino de Investigación Científica y Tecnológica
Repositorio:Repositorio Institucional del IPICYT
OAI Identifier:oai:ipicyt.repositorioinstitucional.mx:1010/2067
Acceso en línea:http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2067
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autor/Biohydrogen
info:eu-repo/classification/Autor/Dark fermentation
info:eu-repo/classification/Autor/Lactic acid bacteria (LAB)
info:eu-repo/classification/Autor/Hydrogen-producing bacteria (HPB)
info:eu-repo/classification/Autor/Microbial community
info:eu-repo/classification/cti/6
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spelling Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactorRodolfo Palomo BrionesEric TrablyNGUYEN ESMERALDA LOPEZ LOZANOMaría de Lourdes Berenice Celis GarcíaHugo Óscar Méndez AcostaNicolas BERNETElías Razo Floresinfo:eu-repo/classification/Autor/Biohydrogeninfo:eu-repo/classification/Autor/Dark fermentationinfo:eu-repo/classification/Autor/Lactic acid bacteria (LAB)info:eu-repo/classification/Autor/Hydrogen-producing bacteria (HPB)info:eu-repo/classification/Autor/Microbial communityinfo:eu-repo/classification/cti/6info:eu-repo/classification/cti/6"The hydrogen (H2) production efficiency in dark fermentation systems is strongly dependent on the occurrence of metabolic pathways derived from the selection of microbial species that either consume molecular H2 or outcompete hydrogenogenic bacteria for the organic substrate. In this study, the effect of organic loading rate (OLR) on the H2 production performance, the metabolic pathways, and the microbial community composition in a continuous system was evaluated. Two bacterial genera, Clostridium and Streptococcus, were dominant in the microbial community depending on the OLR applied. At low OLR (14.7–44.1 gLactose/L-d), Clostridium sp. was dominant and directed the system towards the acetate-butyrate fermentation pathway, with a maximum H2 yield of 2.14 molH2/molHexose obtained at 29.4 gLactose/L-d. Under such conditions, the volumetric hydrogen production rate (VHPR) was between 3.2 and 11.6 LH2/L-d. In contrast, relatively high OLR (58.8 and 88.2 gLactose/L-d) favored the dominance of Streptococcus sp. as co-dominant microorganism leading to lactate production. Under these conditions, the formate production was also stimulated serving as a strategy to dispose the surplus of reduced molecules (e.g., NADH2+), which theoretically consumed up to 5.72 LH2/L-d. In such scenario, the VHPR was enhanced (13.7–14.5 LH2/L-d) but the H2 yield dropped to a minimum of 0.74 molH2/molHexose at OLR?=?58.8 gLactose/L-d. Overall, this research brings clear evidence of the intrinsic occurrence of metabolic pathways detrimental for biohydrogen production, i.e., lactic acid fermentation and formate production, suggesting the use of low OLR as a strategy to control them."Springer2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionapplication/pdfhttp://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2067reponame:Repositorio Institucional del IPICYTinstname:Instituto Potosino de Investigación Científica y Tecnológicainstacron:IPICYTinfo:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1007/s00253-018-8737-7citation:Palomo-Briones, R., Trably, E., López-Lozano, N.E. et al. Appl Microbiol Biotechnol (2018) 102: 2465. https://doi.org/10.1007/s00253-018-8737-7info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:ipicyt.repositorioinstitucional.mx:1010/20672024-08-28T03:17:48Z
dc.title.none.fl_str_mv Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
title Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
spellingShingle Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
Rodolfo Palomo Briones
info:eu-repo/classification/Autor/Biohydrogen
info:eu-repo/classification/Autor/Dark fermentation
info:eu-repo/classification/Autor/Lactic acid bacteria (LAB)
info:eu-repo/classification/Autor/Hydrogen-producing bacteria (HPB)
info:eu-repo/classification/Autor/Microbial community
info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/6
title_short Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
title_full Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
title_fullStr Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
title_full_unstemmed Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
title_sort Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor
dc.creator.none.fl_str_mv Rodolfo Palomo Briones
Eric Trably
NGUYEN ESMERALDA LOPEZ LOZANO
María de Lourdes Berenice Celis García
Hugo Óscar Méndez Acosta
Nicolas BERNET
Elías Razo Flores
author Rodolfo Palomo Briones
author_facet Rodolfo Palomo Briones
Eric Trably
NGUYEN ESMERALDA LOPEZ LOZANO
María de Lourdes Berenice Celis García
Hugo Óscar Méndez Acosta
Nicolas BERNET
Elías Razo Flores
author_role author
author2 Eric Trably
NGUYEN ESMERALDA LOPEZ LOZANO
María de Lourdes Berenice Celis García
Hugo Óscar Méndez Acosta
Nicolas BERNET
Elías Razo Flores
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/Autor/Biohydrogen
info:eu-repo/classification/Autor/Dark fermentation
info:eu-repo/classification/Autor/Lactic acid bacteria (LAB)
info:eu-repo/classification/Autor/Hydrogen-producing bacteria (HPB)
info:eu-repo/classification/Autor/Microbial community
info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/6
topic info:eu-repo/classification/Autor/Biohydrogen
info:eu-repo/classification/Autor/Dark fermentation
info:eu-repo/classification/Autor/Lactic acid bacteria (LAB)
info:eu-repo/classification/Autor/Hydrogen-producing bacteria (HPB)
info:eu-repo/classification/Autor/Microbial community
info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/6
description "The hydrogen (H2) production efficiency in dark fermentation systems is strongly dependent on the occurrence of metabolic pathways derived from the selection of microbial species that either consume molecular H2 or outcompete hydrogenogenic bacteria for the organic substrate. In this study, the effect of organic loading rate (OLR) on the H2 production performance, the metabolic pathways, and the microbial community composition in a continuous system was evaluated. Two bacterial genera, Clostridium and Streptococcus, were dominant in the microbial community depending on the OLR applied. At low OLR (14.7–44.1 gLactose/L-d), Clostridium sp. was dominant and directed the system towards the acetate-butyrate fermentation pathway, with a maximum H2 yield of 2.14 molH2/molHexose obtained at 29.4 gLactose/L-d. Under such conditions, the volumetric hydrogen production rate (VHPR) was between 3.2 and 11.6 LH2/L-d. In contrast, relatively high OLR (58.8 and 88.2 gLactose/L-d) favored the dominance of Streptococcus sp. as co-dominant microorganism leading to lactate production. Under these conditions, the formate production was also stimulated serving as a strategy to dispose the surplus of reduced molecules (e.g., NADH2+), which theoretically consumed up to 5.72 LH2/L-d. In such scenario, the VHPR was enhanced (13.7–14.5 LH2/L-d) but the H2 yield dropped to a minimum of 0.74 molH2/molHexose at OLR?=?58.8 gLactose/L-d. Overall, this research brings clear evidence of the intrinsic occurrence of metabolic pathways detrimental for biohydrogen production, i.e., lactic acid fermentation and formate production, suggesting the use of low OLR as a strategy to control them."
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2067
url http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2067
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1007/s00253-018-8737-7
citation:Palomo-Briones, R., Trably, E., López-Lozano, N.E. et al. Appl Microbiol Biotechnol (2018) 102: 2465. https://doi.org/10.1007/s00253-018-8737-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositorio Institucional del IPICYT
instname:Instituto Potosino de Investigación Científica y Tecnológica
instacron:IPICYT
instname_str Instituto Potosino de Investigación Científica y Tecnológica
instacron_str IPICYT
institution IPICYT
reponame_str Repositorio Institucional del IPICYT
collection Repositorio Institucional del IPICYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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