Muscular myosin isoforms of Taenia solium (Cestoda)

Type II myosin, the primary component of the thick filament of muscle fibers, is organized as a dimeric high molecular weight protein, and is composed of a pair of heavy chains (MHC and two pairs of light chains. Myosin II transforms ATP energy into mechanical force. All type II myosins are conserve...

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Autores: González-Malerva, L., Cruz-Rivera, M., Reynoso-Ducoing, O., Retamal, C., Flisser, A., Ambrosio , JR
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:México
Institución:Universidad Nacional Autónoma de México
Repositorio:Sistema de Información de la Facultad de Ciencias, UNAM
OAI Identifier:oai:repositorio.fciencias.unam.mx:11154/174494
Acceso en línea:http://hdl.handle.net/11154/99956
http://hdl.handle.net/11154/174494
Access Level:acceso abierto
Palabra clave:1254
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spelling Muscular myosin isoforms of Taenia solium (Cestoda)González-Malerva, L.Cruz-Rivera, M.Reynoso-Ducoing, O.Retamal, C.Flisser, A.Ambrosio , JR1254Type II myosin, the primary component of the thick filament of muscle fibers, is organized as a dimeric high molecular weight protein, and is composed of a pair of heavy chains (MHC and two pairs of light chains. Myosin II transforms ATP energy into mechanical force. All type II myosins are conserved proteins but they have two variable regions that are located in different places of the molecule. Myosin molecules are encoded by a multigene family and many isoforms are generated. The expression of myosins depends on the developmental stage and on the type and degree of contractile activity and tissue, therefore several myosin isoforms are found in the same organism. Here we describe the use of different techniques that allowed demonstrating the presence of isoforms of the heavy chain type II myosin of Taenia solilan cysticerci (larvae) and tapeworms (adults), a cestode parasite of importance in public health in many developing countries. Myosin was purified and used in comparative proteolytic fragmentation, ATPase activity, detection of antigenic differences and electrophoretic separation. The results obtained showed biochemical and immunochemical differences among cysticerci and tapeworms, and demonstrate the presence of myosin isoforms in T. solium that are probably associated to physiological requirements of each developmental stage. (C) 2004 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.Cell Biology International2011-12-07T13:24:52Z2014-01-28T15:17:01Z2011-12-07T13:24:52Z2014-01-28T15:17:01Z2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGonzález-Malerva, L; Cruz-Rivera, M; Reynoso-Ducoing, O; Retamal, C; Flisser, A; Ambrosio , JR. (2004). Muscular myosin isoforms of Taenia solium (Cestoda). Cell Biology International, 28(12), 885-894.1065-6995http://hdl.handle.net/11154/9995610.1016/j.cellbi.2004.09.008http://hdl.handle.net/11154/174494reponame:Sistema de Información de la Facultad de Ciencias, UNAMinstname:Universidad Nacional Autónoma de Méxicoinstacron:UNAMenWoSinfo:eu-repo/semantics/openAccessoai:repositorio.fciencias.unam.mx:11154/1744942025-09-17T19:37:29Z
dc.title.none.fl_str_mv Muscular myosin isoforms of Taenia solium (Cestoda)
title Muscular myosin isoforms of Taenia solium (Cestoda)
spellingShingle Muscular myosin isoforms of Taenia solium (Cestoda)
González-Malerva, L.
1254
title_short Muscular myosin isoforms of Taenia solium (Cestoda)
title_full Muscular myosin isoforms of Taenia solium (Cestoda)
title_fullStr Muscular myosin isoforms of Taenia solium (Cestoda)
title_full_unstemmed Muscular myosin isoforms of Taenia solium (Cestoda)
title_sort Muscular myosin isoforms of Taenia solium (Cestoda)
dc.creator.none.fl_str_mv González-Malerva, L.
Cruz-Rivera, M.
Reynoso-Ducoing, O.
Retamal, C.
Flisser, A.
Ambrosio , JR
author González-Malerva, L.
author_facet González-Malerva, L.
Cruz-Rivera, M.
Reynoso-Ducoing, O.
Retamal, C.
Flisser, A.
Ambrosio , JR
author_role author
author2 Cruz-Rivera, M.
Reynoso-Ducoing, O.
Retamal, C.
Flisser, A.
Ambrosio , JR
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 1254
topic 1254
description Type II myosin, the primary component of the thick filament of muscle fibers, is organized as a dimeric high molecular weight protein, and is composed of a pair of heavy chains (MHC and two pairs of light chains. Myosin II transforms ATP energy into mechanical force. All type II myosins are conserved proteins but they have two variable regions that are located in different places of the molecule. Myosin molecules are encoded by a multigene family and many isoforms are generated. The expression of myosins depends on the developmental stage and on the type and degree of contractile activity and tissue, therefore several myosin isoforms are found in the same organism. Here we describe the use of different techniques that allowed demonstrating the presence of isoforms of the heavy chain type II myosin of Taenia solilan cysticerci (larvae) and tapeworms (adults), a cestode parasite of importance in public health in many developing countries. Myosin was purified and used in comparative proteolytic fragmentation, ATPase activity, detection of antigenic differences and electrophoretic separation. The results obtained showed biochemical and immunochemical differences among cysticerci and tapeworms, and demonstrate the presence of myosin isoforms in T. solium that are probably associated to physiological requirements of each developmental stage. (C) 2004 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
publishDate 2004
dc.date.none.fl_str_mv 2004
2011-12-07T13:24:52Z
2011-12-07T13:24:52Z
2014-01-28T15:17:01Z
2014-01-28T15:17:01Z
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv González-Malerva, L; Cruz-Rivera, M; Reynoso-Ducoing, O; Retamal, C; Flisser, A; Ambrosio , JR. (2004). Muscular myosin isoforms of Taenia solium (Cestoda). Cell Biology International, 28(12), 885-894.
1065-6995
http://hdl.handle.net/11154/99956
10.1016/j.cellbi.2004.09.008
http://hdl.handle.net/11154/174494
identifier_str_mv González-Malerva, L; Cruz-Rivera, M; Reynoso-Ducoing, O; Retamal, C; Flisser, A; Ambrosio , JR. (2004). Muscular myosin isoforms of Taenia solium (Cestoda). Cell Biology International, 28(12), 885-894.
1065-6995
10.1016/j.cellbi.2004.09.008
url http://hdl.handle.net/11154/99956
http://hdl.handle.net/11154/174494
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv WoS
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cell Biology International
publisher.none.fl_str_mv Cell Biology International
dc.source.none.fl_str_mv reponame:Sistema de Información de la Facultad de Ciencias, UNAM
instname:Universidad Nacional Autónoma de México
instacron:UNAM
instname_str Universidad Nacional Autónoma de México
instacron_str UNAM
institution UNAM
reponame_str Sistema de Información de la Facultad de Ciencias, UNAM
collection Sistema de Información de la Facultad de Ciencias, UNAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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