Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca
Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | México |
| Institución: | Universidad Veracruzana |
| Repositorio: | Redalyc-UV |
| OAI Identifier: | oai:redalyc.org:44965759006 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=44965759006 https://www.redalyc.org/journal/449/44965759006/ https://www.redalyc.org/journal/449/44965759006/html/ https://www.redalyc.org/journal/449/44965759006/44965759006.epub https://www.redalyc.org/journal/449/44965759006/movil |
| Access Level: | acceso abierto |
| Palabra clave: | Biología nptII Common bean tumefaciens gusA activit recalcitrant species |
| Sumario: | Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene. |
|---|