Antibiotics incorporation in Artemia franciscana nauplii, metanauplii, juveniles and adults, and their inhibitory action on Aeromonas hydrophila bacteria
The crustacean Artemia franciscana has been used as a drug carrier, mainly in its nauplius stage; however, theuse of other developmental stages, i.e., metanauplius, juvenile, and adult, potentially allows treating diseases notonly in fry but also in juveniles and adults. In the present work, we stud...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Redalyc-UAM |
| OAI Identifier: | oai:redalyc.org:57811706 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=57811706 |
| Access Level: | acceso abierto |
| Palabra clave: | Biología Artemia antibiotics bioencapsulation Aeromonas hydrophila |
| Sumario: | The crustacean Artemia franciscana has been used as a drug carrier, mainly in its nauplius stage; however, theuse of other developmental stages, i.e., metanauplius, juvenile, and adult, potentially allows treating diseases notonly in fry but also in juveniles and adults. In the present work, we studied the incorporation of antibiotics in thesestages to inhibit the growth of the bacterium Aeromonas hydrophila, which causes a high mortality in freshwaterfishes. The antibiotics used were: chloramphenicol (base antibiotic), nitrofurantoin (Macrodantina®, 50 mg capsules)and ciprofloxacin (Ciproflox®, 250 mg capsules). Four wells were made in Petri dishes with Trypticase® soybeanagar (TSA) and 2 mL of bacterial inoculum. The wells were used for each antibiotic and one for the control. A 1-mL sample of each Artemia stage, incorporated with an antibiotic, was placed in each well and incubated for 24 hat 37°C, measuring the inhibition halos thereafter. Results indicated that 4 h are needed for the nauplii to becomesaturated and for the metanauplii, juveniles, and adults to fill their digestive tract with the antibiotic. In nauplii, thethree antibiotics produced inhibition halos; in metanauplii, ciprofloxacin produced the best result (22.57 mm); injuveniles, chloramphenicol (38 mm) and ciprofloxacin (33 mm) gave the best results; in adults, the best results wereobtained also with chloramphenicol (33 mm) and ciprofloxacin (40 mm). Nitrofurantoin did not yield positive results inmetanauplii, juveniles, and adults, and because it is soluble in water, it is recommended to apply it in lipidic solutionsto ease its incorporation. Results from this study allow us to establish the bases for the control and treatment ofinfectious diseases caused by the bacterium Aeromonas hydrophila through the use of commercial antibiotics, easilyavailable in Mexico. |
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