Enhancing saccharification of Agave tequilana bagasse by oxidative delignification and enzymatic synergism for the production of hydrogen and methane
"Agave tequilana bagasse is a suitable lignocellulosic residue for energy production. However, the presence of lignin and the heterogeneous structure of hemicellulose may hinder the availability of polysaccharides. In this work, the pretreatment of A. tequilana bagasse with alkaline hydrogen pe...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión enviada para evaluación y publicación |
| Fecha de publicación: | 2018 |
| País: | México |
| Institución: | Instituto Potosino de Investigación Científica y Tecnológica |
| Repositorio: | Repositorio Institucional del IPICYT |
| OAI Identifier: | oai:ipicyt.repositorioinstitucional.mx:1010/2075 |
| Acceso en línea: | http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2075 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/Autor/Agave bagasse info:eu-repo/classification/Autor/Anaerobic digestion info:eu-repo/classification/Autor/Dark fermentation info:eu-repo/classification/Autor/Delignification info:eu-repo/classification/Autor/Enzymatic synergism info:eu-repo/classification/Autor/Lignin info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/23 info:eu-repo/classification/cti/2307 info:eu-repo/classification/cti/221005 |
| Sumario: | "Agave tequilana bagasse is a suitable lignocellulosic residue for energy production. However, the presence of lignin and the heterogeneous structure of hemicellulose may hinder the availability of polysaccharides. In this work, the pretreatment of A. tequilana bagasse with alkaline hydrogen peroxide (AHP) followed by enzymatic saccharification with hemicellulases and cellulases was assessed for the removal of lignin and extraction of fermentable sugars, respectively. Results of the AHP pretreatment indicated that it is possible to attain up to 97% delignification and recover 88% of cellulose and hemicellulose after only 1.5 h of treatment. Regarding the saccharification process, the total sugar yield and productivity were both increased by 2-fold using an enzymatic mixture (cellulases + hemicellulases) compared to single enzyme hydrolysis (cellulases), evidencing synergism. Further evaluation of the hydrolyzates as substrate for hydrogen and methane production, resulted in yields 1.5 and 3.6-times (215.14 ± 13 L H2 and 393.4 ± 13 L CH4 per kg bagasse, respectively) superior to those obtained with hydrolyzates of non-pretreated bagasse processed with a single enzyme. Overall, using AHP pretreatment and subsequent hydrolysis with enzymatic mixtures improves the saccharification of A. tequilana bagasse enhancing the production of hydrogen and methane." |
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