Estudio morfométrico y taxonómico de dinoflagelados del género Ceratium (Neoceratium), con énfasis en N. balechii
The morphometric study is a tool for delimitation of species, however in Dinoflagellates is complicated and demand a big labour under microscopy. Works previous about Ornithocercus and Prorocentrum genus, both they are part of the “Biología y Filogenia Molecular de Dinoflagelados del Pacifico Mexica...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:6d56zw83b |
| Acceso en línea: | https://doi.org/10.24275/uami.6d56zw83b |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Dinoflagellates -- Classification info:eu-repo/classification/LEM/Dinoflagelados -- Clasificación info:eu-repo/classification/LEM/Morphometry info:eu-repo/classification/LEM/Morfometría info:eu-repo/classification/LEM/Biology -- Classification info:eu-repo/classification/LEM/Biología -- Clasificación info:eu-repo/classification/cti/2 |
| Sumario: | The morphometric study is a tool for delimitation of species, however in Dinoflagellates is complicated and demand a big labour under microscopy. Works previous about Ornithocercus and Prorocentrum genus, both they are part of the “Biología y Filogenia Molecular de Dinoflagelados del Pacifico Mexicano” proyect, who is funded by the National Council of Science and Technology (CONACyT) and who point the importance about morphometric studies. This research about some species complex is part of this proyect too, which aims to obtain morphological characters and the internal transcribed spacer (ITS) region that enable the distinction between a complex of species of the genus Neoceratium. N. balechii, N. divaricatum and N. longipes was included in a morphometric study use eight continuos characters in 1022 organism: length total (lt), transdiameter (t), apical horn length (lca), both antapical horns length (lcder and lcizq), distance between both horns antapical (dseca) and distance between hipotecal base to cingulum (dbhc). Analysed with statistics technical (bi and multivariate): Analysis of Variance (ANOVA), Dendrogam (Cluster), Principal Components Analysis (PCA) and Discriminant Analysis (DA) with NCSS program. The finality of use this analysis are to know variation, importance and diagnosis value of importance of characters on delimitation of species. To obtain ITS protocol was performed according to the technique "single cell PCR"described by different authors using primers F 5 'CGT AGG TGA ACC TGC GGA AGG ATC A 3', R 5 'TTC CAC CCT GTT CCG TTA TCG CT 3 'and 5'-A AGC CCA GTA ATC ACA TTC TAG AGG (ACT) TCC GTA GGT-3', 5'-CCTGCAGTCGACA B (TG) ATGCTTAA (AG) TTCAG C (AG) GG-3 '. All characters had statistic signification with P’s˂ 0.00005 DA show statistic signification with three first discriminant functions (P’s˂ 0.00005 y λWilks=0.42759), variables more important in species separation are dbhc, lcder, lcizq y rc. While lt, lca y dseca are the most important in separation forms of C. balechii. It is concluded that the species N. balechii, N. divaricatum and N. longipes are different and distinct. The eight characters used are sufficient and diagnostics for the delimitation of these species. Although the forms of N. balechii statistically significant differences, supporting the hypothesis that both forms are independent of other conspecific taxa because the characters are separated by which respond mainly to the size of these. Because the sequences of ITS regions were unable to obtain this report shows the great importance of the implementation of a large morphometric study. |
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