Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Anthracnose caused by the hemibiotroph fungus Colletotrichum gloeosporioides is a devastating plant disease with an extensive impact on plant productivity. The process of colonization and disease progression of C. gloeosporioides has been studied in a number of angiosperm crops. To better understand...

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Autores: ADRIANA OTERO BLANCA, YORDANIS PEREZ LLANO, VERONICA LIRA RUAN, Jorge Luis Folch Mallol, MARIA DEL RAYO SANCHEZ CARBENTE, RAMON ALBERTO BATISTA GARCIA
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:México
Institución:Universidad Autónoma del Estado de Morelos
Repositorio:Repositorio Institucional de Acceso Abierto de la Universidad Autónoma del Estado de Morelos
Idioma:inglés
OAI Identifier:oai:http://riaa.uaem.mx:20.500.12055/3215
Acceso en línea:http://riaa.uaem.mx/handle/20.500.12055/3215
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
Physcomitrium (Physcomitrella) patens, Colletotrichum gloeosporioides, fungal–bryophyte interaction, transcriptomics; phenomics
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spelling Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA SequencingADRIANA OTERO BLANCAYORDANIS PEREZ LLANOVERONICA LIRA RUANJorge Luis Folch MallolMARIA DEL RAYO SANCHEZ CARBENTERAMON ALBERTO BATISTA GARCIAinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/24Physcomitrium (Physcomitrella) patens, Colletotrichum gloeosporioides, fungal–bryophyte interaction, transcriptomics; phenomicsAnthracnose caused by the hemibiotroph fungus Colletotrichum gloeosporioides is a devastating plant disease with an extensive impact on plant productivity. The process of colonization and disease progression of C. gloeosporioides has been studied in a number of angiosperm crops. To better understand the evolution of the plant response to pathogens, the study of this complex interaction has been extended to bryophytes. The model moss Physcomitrium patens Hedw. B&S (former Physcomitrella patens) is sensitive to known bacterial and fungal phytopathogens, including C. gloeosporioides, which cause infection and cell death. P. patens responses to these microorganisms resemble that of the angiosperms. However, the molecular events during the interaction of P. patens and C. gloeosporioides have not been explored. In this work, we present a comprehensive approach using microscopy, phenomics and RNA-seq analysis to explore the defense response of P. patens to C. gloeosporioides. Microscopy analysis showed that appressoria are already formed at 24 h after inoculation (hai) and tissue colonization and cell death occur at 24 hai and is massive at 48 hai. Consequently, the phenomics analysis showed progressing browning of moss tissues and impaired photosynthesis from 24 to 48 hai. The transcriptomic analysis revealed that more than 1200 P. patens genes were differentially expressed in response to Colletotrichum infection. The analysis of differentially expressed gene function showed that the C. gloeosporioides infection led to a transcription reprogramming in P. patens that upregulated the genes related to pathogen recognition, secondary metabolism, cell wall reinforcement and regulation of gene expression. In accordance with the observed phenomics results, some photosynthesis and chloroplast-related genes were repressed, indicating that, under attack, P. patens changes its transcription from primary metabolism to defend itself from the pathogen.Journal of Fungi2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion2309-608Xhttp://riaa.uaem.mx/handle/20.500.12055/3215reponame:Repositorio Institucional de Acceso Abierto de la Universidad Autónoma del Estado de Morelosinstname:Universidad Autónoma del Estado de Morelosinstacron:UAEMengJournal of Fungi6777https://www.mdpi.com/2309-608X/7/8/677info:eu-repo/semantics/altIdentifier/issn/2309-608XMEXinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0oai:http://riaa.uaem.mx:20.500.12055/32152024-08-28T03:36:42Z
dc.title.none.fl_str_mv Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
title Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
spellingShingle Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
ADRIANA OTERO BLANCA
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
Physcomitrium (Physcomitrella) patens, Colletotrichum gloeosporioides, fungal–bryophyte interaction, transcriptomics; phenomics
title_short Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
title_full Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
title_fullStr Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
title_full_unstemmed Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
title_sort Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing
dc.creator.none.fl_str_mv ADRIANA OTERO BLANCA
YORDANIS PEREZ LLANO
VERONICA LIRA RUAN
Jorge Luis Folch Mallol
MARIA DEL RAYO SANCHEZ CARBENTE
RAMON ALBERTO BATISTA GARCIA
author ADRIANA OTERO BLANCA
author_facet ADRIANA OTERO BLANCA
YORDANIS PEREZ LLANO
VERONICA LIRA RUAN
Jorge Luis Folch Mallol
MARIA DEL RAYO SANCHEZ CARBENTE
RAMON ALBERTO BATISTA GARCIA
author_role author
author2 YORDANIS PEREZ LLANO
VERONICA LIRA RUAN
Jorge Luis Folch Mallol
MARIA DEL RAYO SANCHEZ CARBENTE
RAMON ALBERTO BATISTA GARCIA
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
Physcomitrium (Physcomitrella) patens, Colletotrichum gloeosporioides, fungal–bryophyte interaction, transcriptomics; phenomics
topic info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
Physcomitrium (Physcomitrella) patens, Colletotrichum gloeosporioides, fungal–bryophyte interaction, transcriptomics; phenomics
description Anthracnose caused by the hemibiotroph fungus Colletotrichum gloeosporioides is a devastating plant disease with an extensive impact on plant productivity. The process of colonization and disease progression of C. gloeosporioides has been studied in a number of angiosperm crops. To better understand the evolution of the plant response to pathogens, the study of this complex interaction has been extended to bryophytes. The model moss Physcomitrium patens Hedw. B&S (former Physcomitrella patens) is sensitive to known bacterial and fungal phytopathogens, including C. gloeosporioides, which cause infection and cell death. P. patens responses to these microorganisms resemble that of the angiosperms. However, the molecular events during the interaction of P. patens and C. gloeosporioides have not been explored. In this work, we present a comprehensive approach using microscopy, phenomics and RNA-seq analysis to explore the defense response of P. patens to C. gloeosporioides. Microscopy analysis showed that appressoria are already formed at 24 h after inoculation (hai) and tissue colonization and cell death occur at 24 hai and is massive at 48 hai. Consequently, the phenomics analysis showed progressing browning of moss tissues and impaired photosynthesis from 24 to 48 hai. The transcriptomic analysis revealed that more than 1200 P. patens genes were differentially expressed in response to Colletotrichum infection. The analysis of differentially expressed gene function showed that the C. gloeosporioides infection led to a transcription reprogramming in P. patens that upregulated the genes related to pathogen recognition, secondary metabolism, cell wall reinforcement and regulation of gene expression. In accordance with the observed phenomics results, some photosynthesis and chloroplast-related genes were repressed, indicating that, under attack, P. patens changes its transcription from primary metabolism to defend itself from the pathogen.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 2309-608X
http://riaa.uaem.mx/handle/20.500.12055/3215
identifier_str_mv 2309-608X
url http://riaa.uaem.mx/handle/20.500.12055/3215
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Fungi
677
7
https://www.mdpi.com/2309-608X/7/8/677
info:eu-repo/semantics/altIdentifier/issn/2309-608X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
dc.coverage.none.fl_str_mv MEX
dc.publisher.none.fl_str_mv Journal of Fungi
publisher.none.fl_str_mv Journal of Fungi
dc.source.none.fl_str_mv reponame:Repositorio Institucional de Acceso Abierto de la Universidad Autónoma del Estado de Morelos
instname:Universidad Autónoma del Estado de Morelos
instacron:UAEM
instname_str Universidad Autónoma del Estado de Morelos
instacron_str UAEM
institution UAEM
reponame_str Repositorio Institucional de Acceso Abierto de la Universidad Autónoma del Estado de Morelos
collection Repositorio Institucional de Acceso Abierto de la Universidad Autónoma del Estado de Morelos
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repository.mail.fl_str_mv
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