Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus

Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem bark and roots again...

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Autores: Tania Ortiz López, ROCIO DE LOURDES BORGES ARGAEZ, Guadalupe Ayora_Talavera, Ernesto Alfonso Canto Ramírez, LISSETH ABIGAIL CETINA MONTEJO, ANGEL DE JESUS MAY MAY, Fabiola Escalante Erosa, MIRBELLA DEL ROSARIO CACERES FARFAN
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:México
Institución:Centro de Investigación Científica de Yucatán
Repositorio:Repositorio Institucional CICY
Idioma:inglés
OAI Identifier:oai:cicy.repositorioinstitucional.mx:1003/2671
Acceso en línea:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2671
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autores/ERYTHROSTEMON YUCATANENSIS
info:eu-repo/classification/Autores/INFLUENZA A H1N1 VIRUS
info:eu-repo/classification/Autores/ANTI-HEMAGGLUTININ
info:eu-repo/classification/Autores/STEROLS
info:eu-repo/classification/Autores/FLAVONE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2403
info:eu-repo/classification/cti/230221
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spelling Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 VirusTania Ortiz LópezROCIO DE LOURDES BORGES ARGAEZGuadalupe Ayora_TalaveraErnesto Alfonso Canto RamírezLISSETH ABIGAIL CETINA MONTEJOANGEL DE JESUS MAY MAYFabiola Escalante ErosaMIRBELLA DEL ROSARIO CACERES FARFANinfo:eu-repo/classification/Autores/ERYTHROSTEMON YUCATANENSISinfo:eu-repo/classification/Autores/INFLUENZA A H1N1 VIRUSinfo:eu-repo/classification/Autores/ANTI-HEMAGGLUTININinfo:eu-repo/classification/Autores/STEROLSinfo:eu-repo/classification/Autores/FLAVONEinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/24info:eu-repo/classification/cti/2403info:eu-repo/classification/cti/230221info:eu-repo/classification/cti/230221Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem bark and roots against two strains of the AH1N1 influenza virus was performed, leading to the identification of bioactive compounds in this medicinal plant. In a cytopathic effect reduction assay, the fractions from the leaves and stem bark were the active elements at the co-treatment level. These were further fractionated based on their hemagglutination inhibition activity. The analysis of spectroscopy data identified a combination of phytosterols (β-sitosterol, stigmasterol and campesterol) in the stem bark active fraction as the main anti-hemagglutinin binding components, while 5-hydroxy-2(2-hydroxy-3,4,5-trimethoxyphenyl)-7-metoxi-4H(chromen-4-ona), which was isolated from the leaf extracts, showed a weak inhibition of viral hemagglutinin. Time of addition experiments demonstrated that the mixture of sterols had a direct effect on viral particle infectivity at the co-treatment level (IC50 = 3.125 µg/mL). This effect was also observed in the virus plaque formation inhibition assay, where the mixture showed 90% inhibition in the first 20 min of co-treatment at the same concentration. Additionally, it was found using qRT-PCR that the NP copy number was reduced by 92.85% after 60 min of co-treatment. These results are the first report of components with anti-hemagglutinin binding activity in the genus Erythrostemon sp. © 2022 by the authors.2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/2671Molecules 2022, 27, 5494. https://doi.org/10.3390/molecules27175494reponame:Repositorio Institucional CICYinstname:Centro de Investigación Científica de Yucatáninstacron:CICYenginfo:eu-repo/semantics/altIdentifier/DOI/doi: 10.3390/molecules27175494citation:Ortiz-López, T.; Borges-Argáez, R.; Ayora-Talavera, G.; Canto-Ramírez, E.; Cetina-Montejo, L.; May-May, Á.; Escalante-Erosa, F.; Cáceres-Farfán, M. Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus. Molecules 2022, 27, 5494. https://doi.org/10.3390/molecules27175494info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:cicy.repositorioinstitucional.mx:1003/26712024-08-28T03:19:16Z
dc.title.none.fl_str_mv Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
title Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
spellingShingle Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
Tania Ortiz López
info:eu-repo/classification/Autores/ERYTHROSTEMON YUCATANENSIS
info:eu-repo/classification/Autores/INFLUENZA A H1N1 VIRUS
info:eu-repo/classification/Autores/ANTI-HEMAGGLUTININ
info:eu-repo/classification/Autores/STEROLS
info:eu-repo/classification/Autores/FLAVONE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2403
info:eu-repo/classification/cti/230221
info:eu-repo/classification/cti/230221
title_short Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
title_full Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
title_fullStr Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
title_full_unstemmed Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
title_sort Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
dc.creator.none.fl_str_mv Tania Ortiz López
ROCIO DE LOURDES BORGES ARGAEZ
Guadalupe Ayora_Talavera
Ernesto Alfonso Canto Ramírez
LISSETH ABIGAIL CETINA MONTEJO
ANGEL DE JESUS MAY MAY
Fabiola Escalante Erosa
MIRBELLA DEL ROSARIO CACERES FARFAN
author Tania Ortiz López
author_facet Tania Ortiz López
ROCIO DE LOURDES BORGES ARGAEZ
Guadalupe Ayora_Talavera
Ernesto Alfonso Canto Ramírez
LISSETH ABIGAIL CETINA MONTEJO
ANGEL DE JESUS MAY MAY
Fabiola Escalante Erosa
MIRBELLA DEL ROSARIO CACERES FARFAN
author_role author
author2 ROCIO DE LOURDES BORGES ARGAEZ
Guadalupe Ayora_Talavera
Ernesto Alfonso Canto Ramírez
LISSETH ABIGAIL CETINA MONTEJO
ANGEL DE JESUS MAY MAY
Fabiola Escalante Erosa
MIRBELLA DEL ROSARIO CACERES FARFAN
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/Autores/ERYTHROSTEMON YUCATANENSIS
info:eu-repo/classification/Autores/INFLUENZA A H1N1 VIRUS
info:eu-repo/classification/Autores/ANTI-HEMAGGLUTININ
info:eu-repo/classification/Autores/STEROLS
info:eu-repo/classification/Autores/FLAVONE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2403
info:eu-repo/classification/cti/230221
info:eu-repo/classification/cti/230221
topic info:eu-repo/classification/Autores/ERYTHROSTEMON YUCATANENSIS
info:eu-repo/classification/Autores/INFLUENZA A H1N1 VIRUS
info:eu-repo/classification/Autores/ANTI-HEMAGGLUTININ
info:eu-repo/classification/Autores/STEROLS
info:eu-repo/classification/Autores/FLAVONE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2403
info:eu-repo/classification/cti/230221
info:eu-repo/classification/cti/230221
description Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem bark and roots against two strains of the AH1N1 influenza virus was performed, leading to the identification of bioactive compounds in this medicinal plant. In a cytopathic effect reduction assay, the fractions from the leaves and stem bark were the active elements at the co-treatment level. These were further fractionated based on their hemagglutination inhibition activity. The analysis of spectroscopy data identified a combination of phytosterols (β-sitosterol, stigmasterol and campesterol) in the stem bark active fraction as the main anti-hemagglutinin binding components, while 5-hydroxy-2(2-hydroxy-3,4,5-trimethoxyphenyl)-7-metoxi-4H(chromen-4-ona), which was isolated from the leaf extracts, showed a weak inhibition of viral hemagglutinin. Time of addition experiments demonstrated that the mixture of sterols had a direct effect on viral particle infectivity at the co-treatment level (IC50 = 3.125 µg/mL). This effect was also observed in the virus plaque formation inhibition assay, where the mixture showed 90% inhibition in the first 20 min of co-treatment at the same concentration. Additionally, it was found using qRT-PCR that the NP copy number was reduced by 92.85% after 60 min of co-treatment. These results are the first report of components with anti-hemagglutinin binding activity in the genus Erythrostemon sp. © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2671
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2671
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/DOI/doi: 10.3390/molecules27175494
citation:Ortiz-López, T.; Borges-Argáez, R.; Ayora-Talavera, G.; Canto-Ramírez, E.; Cetina-Montejo, L.; May-May, Á.; Escalante-Erosa, F.; Cáceres-Farfán, M. Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus. Molecules 2022, 27, 5494. https://doi.org/10.3390/molecules27175494
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Molecules 2022, 27, 5494. https://doi.org/10.3390/molecules27175494
reponame:Repositorio Institucional CICY
instname:Centro de Investigación Científica de Yucatán
instacron:CICY
instname_str Centro de Investigación Científica de Yucatán
instacron_str CICY
institution CICY
reponame_str Repositorio Institucional CICY
collection Repositorio Institucional CICY
repository.name.fl_str_mv
repository.mail.fl_str_mv
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