Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations

Collections of Puccinia triticina, the wheat leaf rust pathogen, were obtained from Pakistan in 2008, 2010, 2011, 2013, and 2014. Collections were also obtained from Bhutan in 2013. Single uredinial isolates were derived and tested for virulence phenotype to 20 lines of Thatcher wheat that differ fo...

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Autores: Kolmer, J., Mirza, J.I., Imtiaz, M., Shah, Syed Jawad Ahmad
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2017
País:México
Recursos:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositório:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/20627
Acesso em linha:https://hdl.handle.net/10883/20627
Access Level:Acceso aberto
Palavra-chave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
WHEAT
RUSTS
PUCCINIA RECONDITA
GENETIC MARKERS
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spelling Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populationsKolmer, J.Mirza, J.I.Imtiaz, M.Shah, Syed Jawad AhmadAGRICULTURAL SCIENCES AND BIOTECHNOLOGYWHEATRUSTSPUCCINIA RECONDITAGENETIC MARKERSCollections of Puccinia triticina, the wheat leaf rust pathogen, were obtained from Pakistan in 2008, 2010, 2011, 2013, and 2014. Collections were also obtained from Bhutan in 2013. Single uredinial isolates were derived and tested for virulence phenotype to 20 lines of Thatcher wheat that differ for single leaf rust resistance genes, and for molecular genotype with 23 simple-sequence repeat (SSR) primers. Twenty-four virulence phenotypes were described among the 89 isolates tested for virulence. None of the isolates had virulence to Thatcher lines with Lr9, Lr24, or Lr18. Virulence to most of the other Thatcher lines was over 50%. The two most common virulence phenotypes, FHPSQ and KHPQQ, had virulence to Lr16, Lr17, and Lr26. Twenty-seven SSR genotypes were found among the 38 isolates tested for molecular variation. The SSR genotypes had high levels of observed heterozygosity and significant correlation with virulence phenotype, which indicated clonal reproduction. Cluster analysis and principal component plots indicated three groups of SSR genotypes that also varied significantly for virulence. Isolates with MBDSS and MCDSS virulence phenotypes from Pakistan and Bhutan were highly related for SSR genotype and virulence to isolates from Turkey, Europe, Central Asia, the Middle East, North America and South America, indicating the possible migration of the rust fungus between continental regions.786-790American Phytopathological Society (APS)2020-01-28T14:43:02Z2020-01-28T14:43:02Z2017Published Versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePDFapplication/pdf1943-7684 (Online)0031-949X (Print)https://hdl.handle.net/10883/2062710.1094/PHYTO-10-16-0388-R6107Phytopathologyreponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishUSACIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.Open Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/206272024-10-11T19:55:37Z
dc.title.none.fl_str_mv Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
title Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
spellingShingle Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
Kolmer, J.
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
WHEAT
RUSTS
PUCCINIA RECONDITA
GENETIC MARKERS
title_short Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
title_full Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
title_fullStr Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
title_full_unstemmed Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
title_sort Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Pakistan and genetic relationship to other worldwide populations
dc.creator.none.fl_str_mv Kolmer, J.
Mirza, J.I.
Imtiaz, M.
Shah, Syed Jawad Ahmad
author Kolmer, J.
author_facet Kolmer, J.
Mirza, J.I.
Imtiaz, M.
Shah, Syed Jawad Ahmad
author_role author
author2 Mirza, J.I.
Imtiaz, M.
Shah, Syed Jawad Ahmad
author2_role author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
WHEAT
RUSTS
PUCCINIA RECONDITA
GENETIC MARKERS
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
WHEAT
RUSTS
PUCCINIA RECONDITA
GENETIC MARKERS
description Collections of Puccinia triticina, the wheat leaf rust pathogen, were obtained from Pakistan in 2008, 2010, 2011, 2013, and 2014. Collections were also obtained from Bhutan in 2013. Single uredinial isolates were derived and tested for virulence phenotype to 20 lines of Thatcher wheat that differ for single leaf rust resistance genes, and for molecular genotype with 23 simple-sequence repeat (SSR) primers. Twenty-four virulence phenotypes were described among the 89 isolates tested for virulence. None of the isolates had virulence to Thatcher lines with Lr9, Lr24, or Lr18. Virulence to most of the other Thatcher lines was over 50%. The two most common virulence phenotypes, FHPSQ and KHPQQ, had virulence to Lr16, Lr17, and Lr26. Twenty-seven SSR genotypes were found among the 38 isolates tested for molecular variation. The SSR genotypes had high levels of observed heterozygosity and significant correlation with virulence phenotype, which indicated clonal reproduction. Cluster analysis and principal component plots indicated three groups of SSR genotypes that also varied significantly for virulence. Isolates with MBDSS and MCDSS virulence phenotypes from Pakistan and Bhutan were highly related for SSR genotype and virulence to isolates from Turkey, Europe, Central Asia, the Middle East, North America and South America, indicating the possible migration of the rust fungus between continental regions.
publishDate 2017
dc.date.none.fl_str_mv 2017
2020-01-28T14:43:02Z
2020-01-28T14:43:02Z
dc.type.none.fl_str_mv Published Version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1943-7684 (Online)
0031-949X (Print)
https://hdl.handle.net/10883/20627
10.1094/PHYTO-10-16-0388-R
identifier_str_mv 1943-7684 (Online)
0031-949X (Print)
10.1094/PHYTO-10-16-0388-R
url https://hdl.handle.net/10883/20627
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
dc.coverage.none.fl_str_mv USA
dc.publisher.none.fl_str_mv American Phytopathological Society (APS)
publisher.none.fl_str_mv American Phytopathological Society (APS)
dc.source.none.fl_str_mv 6
107
Phytopathology
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