Endogenous GUS-like activity in Capsicum chinense Jacq.

The gene uidA, codes for β-glucuronidase, which is one of the reporters more frequently utilized in transgenic plants. However, this can only be use if the selected organism does not present endogenous GUS-like activity. In tissues of C. chinense we found a GUS-like activity showing different levels...

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Autores: LAURA YESENIA SOLÍS RAMOS, TOMAS AUGUSTO GONZALEZ ESTRADA, ANTONIO ANDRADE TORRES, GREGORIO DEL CARMEN GODOY HERNANDEZ, ENRIQUE CASTAÑO DE LA SERNA
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:México
Institución:Centro de Investigación Científica de Yucatán
Repositorio:Repositorio Institucional CICY
Idioma:inglés
OAI Identifier:oai:cicy.repositorioinstitucional.mx:1003/1106
Acceso en línea:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1106
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autores/ß-GLUCURONIDASE
info:eu-repo/classification/Autores/AGROBACTERIUM TUMEFACIENS
info:eu-repo/classification/Autores/CAPSICUM CHINENSE
info:eu-repo/classification/Autores/GENE UIDA
info:eu-repo/classification/cti/2
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spelling Endogenous GUS-like activity in Capsicum chinense Jacq.LAURA YESENIA SOLÍS RAMOSTOMAS AUGUSTO GONZALEZ ESTRADAANTONIO ANDRADE TORRESGREGORIO DEL CARMEN GODOY HERNANDEZENRIQUE CASTAÑO DE LA SERNAinfo:eu-repo/classification/Autores/ß-GLUCURONIDASEinfo:eu-repo/classification/Autores/AGROBACTERIUM TUMEFACIENSinfo:eu-repo/classification/Autores/CAPSICUM CHINENSEinfo:eu-repo/classification/Autores/GENE UIDAinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/2The gene uidA, codes for β-glucuronidase, which is one of the reporters more frequently utilized in transgenic plants. However, this can only be use if the selected organism does not present endogenous GUS-like activity. In tissues of C. chinense we found a GUS-like activity showing different levels of intensity. Histochemical screening showed that endogenous GUS-like activitydecreased, or reduced significantly, in almost all tissues with exception of stament, when phosphate buffer was adjusted to pH 8. Subsequently, C. chinense zygotic embryo explants were transient transformed with Agrobacterium tumefaciens LBA4404 (pCAMBIA2301) and plantlets regenerated were histochemically stained in phosphate buffer pH 8. Observations of incubated tissues of C. chinense regenerants showed blue staining, suggesting expression of uidA. Incubated tissues of non-transformed regenerants did not show blue staining in phosphate buffer pH 8. The results show that for transformation experiments of C. chinense with uidA gene, pH 8 is recommended for histochemical staining.2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1106Electronic Journal of Biotechnology, 13(4), 2-3, 2010reponame:Repositorio Institucional CICYinstname:Centro de Investigación Científica de Yucatáninstacron:CICYenginfo:eu-repo/semantics/datasetDOI/DOI: 10.2225/vol13-issue4-fulltext-3citation:Solís-Ramos, L. Y., González-Estrada, T., Andrade-Torres, A., Godoy-Hernández, G., & Castaño de la Serna, E. (2010). Endogenous GUS-like activity in Capsicum chinense Jacq. Electronic Journal of Biotechnology, 13(4), 2-3.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:cicy.repositorioinstitucional.mx:1003/11062024-08-28T03:18:32Z
dc.title.none.fl_str_mv Endogenous GUS-like activity in Capsicum chinense Jacq.
title Endogenous GUS-like activity in Capsicum chinense Jacq.
spellingShingle Endogenous GUS-like activity in Capsicum chinense Jacq.
LAURA YESENIA SOLÍS RAMOS
info:eu-repo/classification/Autores/ß-GLUCURONIDASE
info:eu-repo/classification/Autores/AGROBACTERIUM TUMEFACIENS
info:eu-repo/classification/Autores/CAPSICUM CHINENSE
info:eu-repo/classification/Autores/GENE UIDA
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
title_short Endogenous GUS-like activity in Capsicum chinense Jacq.
title_full Endogenous GUS-like activity in Capsicum chinense Jacq.
title_fullStr Endogenous GUS-like activity in Capsicum chinense Jacq.
title_full_unstemmed Endogenous GUS-like activity in Capsicum chinense Jacq.
title_sort Endogenous GUS-like activity in Capsicum chinense Jacq.
dc.creator.none.fl_str_mv LAURA YESENIA SOLÍS RAMOS
TOMAS AUGUSTO GONZALEZ ESTRADA
ANTONIO ANDRADE TORRES
GREGORIO DEL CARMEN GODOY HERNANDEZ
ENRIQUE CASTAÑO DE LA SERNA
author LAURA YESENIA SOLÍS RAMOS
author_facet LAURA YESENIA SOLÍS RAMOS
TOMAS AUGUSTO GONZALEZ ESTRADA
ANTONIO ANDRADE TORRES
GREGORIO DEL CARMEN GODOY HERNANDEZ
ENRIQUE CASTAÑO DE LA SERNA
author_role author
author2 TOMAS AUGUSTO GONZALEZ ESTRADA
ANTONIO ANDRADE TORRES
GREGORIO DEL CARMEN GODOY HERNANDEZ
ENRIQUE CASTAÑO DE LA SERNA
author2_role author
author
author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/Autores/ß-GLUCURONIDASE
info:eu-repo/classification/Autores/AGROBACTERIUM TUMEFACIENS
info:eu-repo/classification/Autores/CAPSICUM CHINENSE
info:eu-repo/classification/Autores/GENE UIDA
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
topic info:eu-repo/classification/Autores/ß-GLUCURONIDASE
info:eu-repo/classification/Autores/AGROBACTERIUM TUMEFACIENS
info:eu-repo/classification/Autores/CAPSICUM CHINENSE
info:eu-repo/classification/Autores/GENE UIDA
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
description The gene uidA, codes for β-glucuronidase, which is one of the reporters more frequently utilized in transgenic plants. However, this can only be use if the selected organism does not present endogenous GUS-like activity. In tissues of C. chinense we found a GUS-like activity showing different levels of intensity. Histochemical screening showed that endogenous GUS-like activitydecreased, or reduced significantly, in almost all tissues with exception of stament, when phosphate buffer was adjusted to pH 8. Subsequently, C. chinense zygotic embryo explants were transient transformed with Agrobacterium tumefaciens LBA4404 (pCAMBIA2301) and plantlets regenerated were histochemically stained in phosphate buffer pH 8. Observations of incubated tissues of C. chinense regenerants showed blue staining, suggesting expression of uidA. Incubated tissues of non-transformed regenerants did not show blue staining in phosphate buffer pH 8. The results show that for transformation experiments of C. chinense with uidA gene, pH 8 is recommended for histochemical staining.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1106
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1106
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/datasetDOI/DOI: 10.2225/vol13-issue4-fulltext-3
citation:Solís-Ramos, L. Y., González-Estrada, T., Andrade-Torres, A., Godoy-Hernández, G., & Castaño de la Serna, E. (2010). Endogenous GUS-like activity in Capsicum chinense Jacq. Electronic Journal of Biotechnology, 13(4), 2-3.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Electronic Journal of Biotechnology, 13(4), 2-3, 2010
reponame:Repositorio Institucional CICY
instname:Centro de Investigación Científica de Yucatán
instacron:CICY
instname_str Centro de Investigación Científica de Yucatán
instacron_str CICY
institution CICY
reponame_str Repositorio Institucional CICY
collection Repositorio Institucional CICY
repository.name.fl_str_mv
repository.mail.fl_str_mv
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