Producción de ácido cítrico por Aspergillus brasiliensis en fermentación en estado sólido
Citric acid is industrially produced by liquid-state fermentation using fungi of the genus Aspergillus; however, this process heavily relies on the composition of the culture medium. On the other hand, citric acid production by solid-state fermentation is more robust in physiological and nutritional...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:sf268565z |
| Acceso en línea: | https://doi.org/10.24275/uami.sf268565z |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Ácido cítrico info:eu-repo/classification/LEM/Fermentación info:eu-repo/classification/LEM/Fermentation info:eu-repo/classification/LEM/Hogos filamentosos info:eu-repo/classification/LEM/Citric acid info:eu-repo/classification/LEM/Filamentous fungi info:eu-repo/classification/cti/6 |
| Sumario: | Citric acid is industrially produced by liquid-state fermentation using fungi of the genus Aspergillus; however, this process heavily relies on the composition of the culture medium. On the other hand, citric acid production by solid-state fermentation is more robust in physiological and nutritional terms, favoring the growth and enabling the development of more productive processes. Traditionally, solid-state fermentation employs agro-industrial residues; however, this causes operational issues related to the chemical composition of the medium and analytical problems for substrate, biomass, and product recovery analysis. Therefore, an alternative is the use of inert supports such as polyurethane foam, which has a high-water retention capacity (up to 60 times its weight), allowing the recovery of concentrated products without the need to add extraction agents as required with agroindustrial residues as substrates. The use of polyurethane foam impregnated with a defined medium allows for the design of precise cultivation conditions for citric acid production. The objective of this work was to evaluate the effect of the composition of the defined culture medium on citric acid production by A brasiliensis when grown on polyurethane foam, taking advantage of the benefits of solid-state fermentation. For this purpose, a statistical approach based on factorial designs was used to enhance citric acid production. As a result, the maximum yields of citric acid and polyols from glucose as a carbon source were 0.62 Cmol/Cmol and 0.11 Cmol/Cmol respectively. Citric acid production and substrate consumption were negatively affected by the hypertonic concentration of glucose in the medium (>150 mg/mL). Additionally, it was determined that trace elements (Cu2+, Zn2+, Mn2+ y Mo2+) have a negative influence (>0.016 mg/mL) on citric acid, polyols, and biomass production. To the best of our knowledge, this work reports the highest citric acid production yield in solid-state fermentation on polyurethane foam with a defined culture medium. |
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