DIAGNOSIS AND PREVALENCE OF THEILERIA EQUI HORSES IN WESTERN MEXICO BY NESTED PCR
Theileria equi infection prevalence was calculated from 1000 blood samples obtained from apparently healthy horses in western Mexico. Samples were sent to the Animal Biotechnology Laboratory of the University of Guadalajara (Mexico) for T. equi diagnosis. Nested polymerase chain reaction (nPCR) was...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | México |
| Institución: | Universidad Autónoma de Nayarit |
| Repositorio: | Repositorio Institucional Aramara de la UAN |
| Idioma: | inglés |
| OAI Identifier: | oai:dspace.uan.mx:123456789/1974 |
| Acceso en línea: | https://doi.org/10.1016/j.parint.2016.09.011 http://dspace.uan.mx:8080/jspui/handle/123456789/1974 |
| Access Level: | acceso abierto |
| Palabra clave: | Theileria equi Horse Nested polymerase chain reaction Mexico Caballo Reacción en cadena de la polimerasa anidada CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA [6] |
| Sumario: | Theileria equi infection prevalence was calculated from 1000 blood samples obtained from apparently healthy horses in western Mexico. Samples were sent to the Animal Biotechnology Laboratory of the University of Guadalajara (Mexico) for T. equi diagnosis. Nested polymerase chain reaction (nPCR) was used as a diagnostic method to detect pathogen DNA. Using primers for the merozoite antigen-1 (EMA-1) gene, 19.70 ± 2.47% of the horses (95% CI, 17.23–22.17%) tested positive for T. equi. There was no significant association between gender and T. equi infection. However, prevalence was higher among stabled horses (25.81%) than that among grazing horses (15.02%). The positivity rate was also higher among Quarter Horse (24.70%), Lusitano (35.90%), and Costa Rican Saddle Horse (47.37%) breeds than that among the other seven breeds investigated in this study. The percentage of T. equi infection was higher among adult horses (≥ 4 years old, 25.05%) than that among colts and fillies (2–4 years old, 15.48%), yearlings (1–2 years old, 10.49%), and foals (< 1 year old, 10.34%). This is the first study of T. equi infection prevalence among horses in Mexico by nPCR . The results indicate that the equine piroplasmosis (EP) caused by T. equi is enzootic in western Mexico. |
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