Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”

Leaf rust and stripe rust are important wheat diseases worldwide causing significant losses where susceptible varieties are grown. Resistant cultivars offer long-term control and reduce the use of hazardous chemicals, which can be detrimental to both human health and the environment. Land races have...

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Autores: Peipei Zhang, Lan, C., Singh, R.P., Huerta-Espino, J., Zaifeng Li, Lagudah, E., Bhavani, S.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:México
Recursos:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/22253
Acesso em linha:https://hdl.handle.net/10883/22253
Access Level:acceso abierto
Palavra-chave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Analysis
Molecular Mapping
Wheat Rusts
APR Genes
ADULT PLANT RESISTANCE
LANDRACES
WHEAT
RUSTS
id MX_0df56574a4e8c9db8dbee38a2dccc622
oai_identifier_str oai:repository.cimmyt.org:10883/22253
network_acronym_str MX
network_name_str México
repository_id_str
dc.title.none.fl_str_mv Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
title Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
spellingShingle Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
Peipei Zhang
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Analysis
Molecular Mapping
Wheat Rusts
APR Genes
ADULT PLANT RESISTANCE
LANDRACES
WHEAT
RUSTS
title_short Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
title_full Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
title_fullStr Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
title_full_unstemmed Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
title_sort Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”
dc.creator.none.fl_str_mv Peipei Zhang
Lan, C.
Singh, R.P.
Huerta-Espino, J.
Zaifeng Li
Lagudah, E.
Bhavani, S.
author Peipei Zhang
author_facet Peipei Zhang
Lan, C.
Singh, R.P.
Huerta-Espino, J.
Zaifeng Li
Lagudah, E.
Bhavani, S.
author_role author
author2 Lan, C.
Singh, R.P.
Huerta-Espino, J.
Zaifeng Li
Lagudah, E.
Bhavani, S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Analysis
Molecular Mapping
Wheat Rusts
APR Genes
ADULT PLANT RESISTANCE
LANDRACES
WHEAT
RUSTS
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Analysis
Molecular Mapping
Wheat Rusts
APR Genes
ADULT PLANT RESISTANCE
LANDRACES
WHEAT
RUSTS
description Leaf rust and stripe rust are important wheat diseases worldwide causing significant losses where susceptible varieties are grown. Resistant cultivars offer long-term control and reduce the use of hazardous chemicals, which can be detrimental to both human health and the environment. Land races have been a valuable resource for mining new genes for various abiotic and biotic stresses including wheat rusts. Afghan wheat landrace “KU3067” displayed high seedling infection type (IT) for leaf rust and low IT for stripe rust; however, it displayed high levels of field resistance for both rusts when tested for multiple seasons against the Mexican rust isolates. This study focused on identifying loci-conferring seedling resistance to stripe rust, and also loci-conferring adult plant resistance (APR) against the Mexican races of leaf rust and stripe rust. A backcrossed inbred line (BIL) population advanced to the BC1F5 generation derived from the cross of KU3067 and Apav (triple rust susceptible line) was used for both, inheritance and QTL mapping studies. The population and parents were genotyped with Diversity Arrays Technology-genotyping-by-sequencing (DArT-Seq) and phenotyped for leaf rust and stripe rust response at both seedling and adult plant stages during multiple seasons in Mexico with relevant pathotypes. Mapping results identified an all-stage resistance gene for stripe rust, temporarily designated as YrKU, on chromosome 7BL. In total, six QTL-conferring APR to leaf rust on 1AS, 2AL, 4DL, 6BL, 7AL, and 7BL, and four QTL for stripe rust resistance on 1BS, 2AL, 4DL, and 7BL were detected in the analyses. Among these, pleiotropic gene Lr67/Yr46 on 4DL with a significantly large effect is the first report in an Afghan landrace-conferring resistance to both leaf and stripe rusts. QLr.cim-7BL/YrKU showed pleiotropic resistance to both rusts and explained 7.5–17.2 and 12.6–19.3% of the phenotypic variance for leaf and stripe rusts, respectively. QYr.cim-1BS and QYr.cim-2AL detected in all stripe environments with phenotypic variance explained (PVE) 12.9–20.5 and 5.4–12.5%, and QLr.cim-6BL are likely to be new. These QTL and their closely linked markers will be useful for fine mapping and marker-assisted selection (MAS) in breeding for durable resistance to multiple rust diseases.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-11T01:20:13Z
2022-11-11T01:20:13Z
2022
dc.type.none.fl_str_mv Published Version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10883/22253
10.3389/fpls.2022.894528
url https://hdl.handle.net/10883/22253
identifier_str_mv 10.3389/fpls.2022.894528
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.relation.none.fl_str_mv https://figshare.com/collections/Identification_and_Characterization_of_Resistance_Loci_to_Wheat_Leaf_Rust_and_Stripe_Rust_in_Afghan_Landrace_KU3067_/6067598
Nutrition, health & food security
Accelerated Breeding
Genetic Innovation
Australian Grains Research and Development Corporation (GRDC)
Bill & Melinda Gates Foundation (BMGF)
Foreign, Commonwealth & Development Office (FCDO)
United States Agency for International Development (USAID)
Foundation for Food & Agriculture Research (FFAR)
National Natural Science Foundation of China
https://hdl.handle.net/10568/130395
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Switzerland
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv 13
1664-462X
Frontiers in Plant Science
894528
reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
instname:Centro Internacional de Mejoramiento de Maíz y Trigo
instacron:CIMMYT
instname_str Centro Internacional de Mejoramiento de Maíz y Trigo
instacron_str CIMMYT
institution CIMMYT
reponame_str Repositorio Institucional de Publicaciones Multimedia del CIMMYT
collection Repositorio Institucional de Publicaciones Multimedia del CIMMYT
repository.name.fl_str_mv
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spelling Identification and characterization of resistance loci to wheat leaf rust and stripe rust in Afghan landrace “KU3067”Peipei ZhangLan, C.Singh, R.P.Huerta-Espino, J.Zaifeng LiLagudah, E.Bhavani, S.AGRICULTURAL SCIENCES AND BIOTECHNOLOGYGenetic AnalysisMolecular MappingWheat RustsAPR GenesADULT PLANT RESISTANCELANDRACESWHEATRUSTSLeaf rust and stripe rust are important wheat diseases worldwide causing significant losses where susceptible varieties are grown. Resistant cultivars offer long-term control and reduce the use of hazardous chemicals, which can be detrimental to both human health and the environment. Land races have been a valuable resource for mining new genes for various abiotic and biotic stresses including wheat rusts. Afghan wheat landrace “KU3067” displayed high seedling infection type (IT) for leaf rust and low IT for stripe rust; however, it displayed high levels of field resistance for both rusts when tested for multiple seasons against the Mexican rust isolates. This study focused on identifying loci-conferring seedling resistance to stripe rust, and also loci-conferring adult plant resistance (APR) against the Mexican races of leaf rust and stripe rust. A backcrossed inbred line (BIL) population advanced to the BC1F5 generation derived from the cross of KU3067 and Apav (triple rust susceptible line) was used for both, inheritance and QTL mapping studies. The population and parents were genotyped with Diversity Arrays Technology-genotyping-by-sequencing (DArT-Seq) and phenotyped for leaf rust and stripe rust response at both seedling and adult plant stages during multiple seasons in Mexico with relevant pathotypes. Mapping results identified an all-stage resistance gene for stripe rust, temporarily designated as YrKU, on chromosome 7BL. In total, six QTL-conferring APR to leaf rust on 1AS, 2AL, 4DL, 6BL, 7AL, and 7BL, and four QTL for stripe rust resistance on 1BS, 2AL, 4DL, and 7BL were detected in the analyses. Among these, pleiotropic gene Lr67/Yr46 on 4DL with a significantly large effect is the first report in an Afghan landrace-conferring resistance to both leaf and stripe rusts. QLr.cim-7BL/YrKU showed pleiotropic resistance to both rusts and explained 7.5–17.2 and 12.6–19.3% of the phenotypic variance for leaf and stripe rusts, respectively. QYr.cim-1BS and QYr.cim-2AL detected in all stripe environments with phenotypic variance explained (PVE) 12.9–20.5 and 5.4–12.5%, and QLr.cim-6BL are likely to be new. These QTL and their closely linked markers will be useful for fine mapping and marker-assisted selection (MAS) in breeding for durable resistance to multiple rust diseases.Frontiers Media S.A.2022-11-11T01:20:13Z2022-11-11T01:20:13Z2022Published Versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10883/2225310.3389/fpls.2022.894528131664-462XFrontiers in Plant Science894528reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishhttps://figshare.com/collections/Identification_and_Characterization_of_Resistance_Loci_to_Wheat_Leaf_Rust_and_Stripe_Rust_in_Afghan_Landrace_KU3067_/6067598Nutrition, health & food securityAccelerated BreedingGenetic InnovationAustralian Grains Research and Development Corporation (GRDC)Bill & Melinda Gates Foundation (BMGF)Foreign, Commonwealth & Development Office (FCDO)United States Agency for International Development (USAID)Foundation for Food & Agriculture Research (FFAR)National Natural Science Foundation of Chinahttps://hdl.handle.net/10568/130395SwitzerlandCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purposeOpen Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/222532024-10-11T19:59:17Z
score 15,812429