Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya

Maize is the most important food crop in Kenya accounting for more than 51 % of all staples grown in the country. Out of Kenya's 5.3 million ha total crops area, more than 2.1 million ha is occupied by maize which translates to 40 % of all crops area. However, with the emergence of maize lethal...

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Detalles Bibliográficos
Autores: Mwatuni, F., Nyende, A.B., Njuguna, J., Xiong Zhonguo, Machuka, E., Stomeo, F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:México
Institución:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/20931
Acceso en línea:https://hdl.handle.net/10883/20931
Access Level:acceso abierto
Palabra clave:SURVEILLANCE SYSTEMS
PLANT DISEASES
MAIZE CHLOROTIC MOTTLE VIRUS
SUGARCANE MOSAIC VIRUS
RECOMBINATION
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spelling Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in KenyaMwatuni, F.Nyende, A.B.Njuguna, J.Xiong ZhonguoMachuka, E.Stomeo, F.SURVEILLANCE SYSTEMSPLANT DISEASESMAIZE CHLOROTIC MOTTLE VIRUSSUGARCANE MOSAIC VIRUSRECOMBINATIONMaize is the most important food crop in Kenya accounting for more than 51 % of all staples grown in the country. Out of Kenya's 5.3 million ha total crops area, more than 2.1 million ha is occupied by maize which translates to 40 % of all crops area. However, with the emergence of maize lethal necrosis (MLN) disease in 2011, the average yields plummeted to all-time lows with severely affected counties recording 90?100% yield loss in 2013 and 2014. The disease is mainly caused by Maize chlorotic mottle virus (MCMV) in combination with Sugarcane mosaic virus (SCMV) or other potyviruses. In this study, a country-wide survey was carried out to assess the MLN causing viruses in Kenya, their distribution, genetic diversity, and recombination. The causative viruses of MLN were determined by RT-PCR using virus-specific primers and DAS-ELISA. Next-generation sequencing (NGS) data was generated, viral sequences identified, genetic diversity of MLN viruses was determined, and recombination was evaluated. MCMV and SCMV were detected in all the maize growing regions at varying levels of incidence, and severity while MaYMV, a polerovirus was detected in some samples through NGS. However, there were some samples in this study where only MCMV was detected with severe MLN symptoms. SCMV Sequences were highly diverse while MCMV sequences exhibited low variability. Potential recombination events were detected only in SCMV explaining the elevated level of diversity and associated risk of this virus in Kenya and the eastern Africa region.Elsevier2020-08-07T00:05:16Z2020-08-07T00:05:16Z2020Published Versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePDFapplication/pdf0168-1702 (Print)https://hdl.handle.net/10883/2093110.1016/j.virusres.2020.198081286Virus Research198081reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishhttps://www.sciencedirect.com/science/article/pii/S0168170220300034?via%3Dihub#sec0090KENYAAmsterdam (Netherlands)CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.Open Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/209312024-10-11T19:58:10Z
dc.title.none.fl_str_mv Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
title Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
spellingShingle Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
Mwatuni, F.
SURVEILLANCE SYSTEMS
PLANT DISEASES
MAIZE CHLOROTIC MOTTLE VIRUS
SUGARCANE MOSAIC VIRUS
RECOMBINATION
title_short Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
title_full Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
title_fullStr Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
title_full_unstemmed Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
title_sort Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya
dc.creator.none.fl_str_mv Mwatuni, F.
Nyende, A.B.
Njuguna, J.
Xiong Zhonguo
Machuka, E.
Stomeo, F.
author Mwatuni, F.
author_facet Mwatuni, F.
Nyende, A.B.
Njuguna, J.
Xiong Zhonguo
Machuka, E.
Stomeo, F.
author_role author
author2 Nyende, A.B.
Njuguna, J.
Xiong Zhonguo
Machuka, E.
Stomeo, F.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SURVEILLANCE SYSTEMS
PLANT DISEASES
MAIZE CHLOROTIC MOTTLE VIRUS
SUGARCANE MOSAIC VIRUS
RECOMBINATION
topic SURVEILLANCE SYSTEMS
PLANT DISEASES
MAIZE CHLOROTIC MOTTLE VIRUS
SUGARCANE MOSAIC VIRUS
RECOMBINATION
description Maize is the most important food crop in Kenya accounting for more than 51 % of all staples grown in the country. Out of Kenya's 5.3 million ha total crops area, more than 2.1 million ha is occupied by maize which translates to 40 % of all crops area. However, with the emergence of maize lethal necrosis (MLN) disease in 2011, the average yields plummeted to all-time lows with severely affected counties recording 90?100% yield loss in 2013 and 2014. The disease is mainly caused by Maize chlorotic mottle virus (MCMV) in combination with Sugarcane mosaic virus (SCMV) or other potyviruses. In this study, a country-wide survey was carried out to assess the MLN causing viruses in Kenya, their distribution, genetic diversity, and recombination. The causative viruses of MLN were determined by RT-PCR using virus-specific primers and DAS-ELISA. Next-generation sequencing (NGS) data was generated, viral sequences identified, genetic diversity of MLN viruses was determined, and recombination was evaluated. MCMV and SCMV were detected in all the maize growing regions at varying levels of incidence, and severity while MaYMV, a polerovirus was detected in some samples through NGS. However, there were some samples in this study where only MCMV was detected with severe MLN symptoms. SCMV Sequences were highly diverse while MCMV sequences exhibited low variability. Potential recombination events were detected only in SCMV explaining the elevated level of diversity and associated risk of this virus in Kenya and the eastern Africa region.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-07T00:05:16Z
2020-08-07T00:05:16Z
2020
dc.type.none.fl_str_mv Published Version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0168-1702 (Print)
https://hdl.handle.net/10883/20931
10.1016/j.virusres.2020.198081
identifier_str_mv 0168-1702 (Print)
10.1016/j.virusres.2020.198081
url https://hdl.handle.net/10883/20931
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0168170220300034?via%3Dihub#sec0090
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
dc.coverage.none.fl_str_mv KENYA
Amsterdam (Netherlands)
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv 286
Virus Research
198081
reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
instname:Centro Internacional de Mejoramiento de Maíz y Trigo
instacron:CIMMYT
instname_str Centro Internacional de Mejoramiento de Maíz y Trigo
instacron_str CIMMYT
institution CIMMYT
reponame_str Repositorio Institucional de Publicaciones Multimedia del CIMMYT
collection Repositorio Institucional de Publicaciones Multimedia del CIMMYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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