The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWR...

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Autores: SERGIO GARCIA LAYNES, VIRGINIA AURORA HERRERA VALENCIA, Lilia Guadalupe Tamayo Torres, VERONICA LIMONES BRIONES, FELIPE ALONSO BARREDO POOL, FRAY MARTIN BAAS ESPINOLA, Angel Alpuche-Solis, CARLOS ALBERTO PUCH HAU, SANTY PERAZA ECHEVERRIA
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:México
Institución:Centro de Investigación Científica de Yucatán
Repositorio:Repositorio Institucional CICY
Idioma:inglés
OAI Identifier:oai:cicy.repositorioinstitucional.mx:1003/2853
Acceso en línea:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2853
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autores/BANANA
info:eu-repo/classification/Autores/TRANSCRIPTION FACTOR
info:eu-repo/classification/Autores/WRKY
info:eu-repo/classification/Autores/DEFENSE PHYTOHORMONES
info:eu-repo/classification/Autores/SALICYLIC ACID
info:eu-repo/classification/Autores/METHYL JASMONATE
info:eu-repo/classification/Autores/SAR
info:eu-repo/classification/Autores/ISR
info:eu-repo/classification/Autores/BROAD-SPECTRUM RESISTANCE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2409
info:eu-repo/classification/cti/240992
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spelling The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormonesSERGIO GARCIA LAYNESVIRGINIA AURORA HERRERA VALENCIALilia Guadalupe Tamayo TorresVERONICA LIMONES BRIONESFELIPE ALONSO BARREDO POOLFRAY MARTIN BAAS ESPINOLAAngel Alpuche-SolisCARLOS ALBERTO PUCH HAUSANTY PERAZA ECHEVERRIAinfo:eu-repo/classification/Autores/BANANAinfo:eu-repo/classification/Autores/TRANSCRIPTION FACTORinfo:eu-repo/classification/Autores/WRKYinfo:eu-repo/classification/Autores/DEFENSE PHYTOHORMONESinfo:eu-repo/classification/Autores/SALICYLIC ACIDinfo:eu-repo/classification/Autores/METHYL JASMONATEinfo:eu-repo/classification/Autores/SARinfo:eu-repo/classification/Autores/ISRinfo:eu-repo/classification/Autores/BROAD-SPECTRUM RESISTANCEinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/24info:eu-repo/classification/cti/2409info:eu-repo/classification/cti/240992info:eu-repo/classification/cti/240992WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/2853Genes, 13(10), 1891, 2022.reponame:Repositorio Institucional CICYinstname:Centro de Investigación Científica de Yucatáninstacron:CICYenginfo:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/genes13101891citation:García-Laynes, S.; Herrera-Valencia, V.A.; Tamayo-Torres, L.G.; Limones-Briones, V.; Barredo-Pool, F.A.; Baas-Espinola, F.M.; Alpuche-Solís, A.G.; Puch-Hau, C.; Peraza-Echeverria, S. The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones. Genes 2022, 13, 1891. https://doi.org/10.3390/genes13101891info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:cicy.repositorioinstitucional.mx:1003/28532024-08-28T03:19:29Z
dc.title.none.fl_str_mv The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
title The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
spellingShingle The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
SERGIO GARCIA LAYNES
info:eu-repo/classification/Autores/BANANA
info:eu-repo/classification/Autores/TRANSCRIPTION FACTOR
info:eu-repo/classification/Autores/WRKY
info:eu-repo/classification/Autores/DEFENSE PHYTOHORMONES
info:eu-repo/classification/Autores/SALICYLIC ACID
info:eu-repo/classification/Autores/METHYL JASMONATE
info:eu-repo/classification/Autores/SAR
info:eu-repo/classification/Autores/ISR
info:eu-repo/classification/Autores/BROAD-SPECTRUM RESISTANCE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2409
info:eu-repo/classification/cti/240992
info:eu-repo/classification/cti/240992
title_short The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
title_full The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
title_fullStr The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
title_full_unstemmed The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
title_sort The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
dc.creator.none.fl_str_mv SERGIO GARCIA LAYNES
VIRGINIA AURORA HERRERA VALENCIA
Lilia Guadalupe Tamayo Torres
VERONICA LIMONES BRIONES
FELIPE ALONSO BARREDO POOL
FRAY MARTIN BAAS ESPINOLA
Angel Alpuche-Solis
CARLOS ALBERTO PUCH HAU
SANTY PERAZA ECHEVERRIA
author SERGIO GARCIA LAYNES
author_facet SERGIO GARCIA LAYNES
VIRGINIA AURORA HERRERA VALENCIA
Lilia Guadalupe Tamayo Torres
VERONICA LIMONES BRIONES
FELIPE ALONSO BARREDO POOL
FRAY MARTIN BAAS ESPINOLA
Angel Alpuche-Solis
CARLOS ALBERTO PUCH HAU
SANTY PERAZA ECHEVERRIA
author_role author
author2 VIRGINIA AURORA HERRERA VALENCIA
Lilia Guadalupe Tamayo Torres
VERONICA LIMONES BRIONES
FELIPE ALONSO BARREDO POOL
FRAY MARTIN BAAS ESPINOLA
Angel Alpuche-Solis
CARLOS ALBERTO PUCH HAU
SANTY PERAZA ECHEVERRIA
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/Autores/BANANA
info:eu-repo/classification/Autores/TRANSCRIPTION FACTOR
info:eu-repo/classification/Autores/WRKY
info:eu-repo/classification/Autores/DEFENSE PHYTOHORMONES
info:eu-repo/classification/Autores/SALICYLIC ACID
info:eu-repo/classification/Autores/METHYL JASMONATE
info:eu-repo/classification/Autores/SAR
info:eu-repo/classification/Autores/ISR
info:eu-repo/classification/Autores/BROAD-SPECTRUM RESISTANCE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2409
info:eu-repo/classification/cti/240992
info:eu-repo/classification/cti/240992
topic info:eu-repo/classification/Autores/BANANA
info:eu-repo/classification/Autores/TRANSCRIPTION FACTOR
info:eu-repo/classification/Autores/WRKY
info:eu-repo/classification/Autores/DEFENSE PHYTOHORMONES
info:eu-repo/classification/Autores/SALICYLIC ACID
info:eu-repo/classification/Autores/METHYL JASMONATE
info:eu-repo/classification/Autores/SAR
info:eu-repo/classification/Autores/ISR
info:eu-repo/classification/Autores/BROAD-SPECTRUM RESISTANCE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2409
info:eu-repo/classification/cti/240992
info:eu-repo/classification/cti/240992
description WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2853
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2853
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/genes13101891
citation:García-Laynes, S.; Herrera-Valencia, V.A.; Tamayo-Torres, L.G.; Limones-Briones, V.; Barredo-Pool, F.A.; Baas-Espinola, F.M.; Alpuche-Solís, A.G.; Puch-Hau, C.; Peraza-Echeverria, S. The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones. Genes 2022, 13, 1891. https://doi.org/10.3390/genes13101891
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Genes, 13(10), 1891, 2022.
reponame:Repositorio Institucional CICY
instname:Centro de Investigación Científica de Yucatán
instacron:CICY
instname_str Centro de Investigación Científica de Yucatán
instacron_str CICY
institution CICY
reponame_str Repositorio Institucional CICY
collection Repositorio Institucional CICY
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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