Aislamiento y caracterización del gen ORF5 del virus del síndrome reproductivo y respiratorio porcino (PRRS) en México
The porcine reproductive and respiratory syndrome (PRRS) represents a major menace to the national and international porcineindustry. The virus genetics and antigenic properties, as well as its capacity to modulate the immune system, have made it dif-fi cult to control this virus. In Mexico, as in t...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2006 |
| País: | México |
| Institución: | Instituto Mexicano del Seguro Social |
| Repositorio: | Redalyc-IMSS |
| OAI Identifier: | oai:redalyc.org:42337205 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=42337205 |
| Access Level: | acceso abierto |
| Palabra clave: | Veterinaria RT ORF PCR VIRUS PRRSV |
| Sumario: | The porcine reproductive and respiratory syndrome (PRRS) represents a major menace to the national and international porcineindustry. The virus genetics and antigenic properties, as well as its capacity to modulate the immune system, have made it dif-fi cult to control this virus. In Mexico, as in the rest of world, the disease produces important economic losses in the porcine industry.This study describes the isolation and characterization of PRRS virus obtained from pigs of a farm located in Sonora, Mexico.The isolated virus was identifi ed by nested RT-PCR of the ORF-6 gene, and by cytopathic effect in MARC -145 cells. The isolatedvirus was inoculated in negative pigs which became viremic and produced antibodies and mononuclear cell (MNC) responseafter inoculation. In order to establish the genotype of the isolated virus, the sequence of the gene ORF-5 was determined, and itwas 88% and 87 % similar to the American virus (VR-2332) and the modifi ed live virus (MLV) vaccine, respectively. These studiesare basic for the development of strategies oriented to control this disease in Mexico. |
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