GENOTOXICITY OF COMMERCIAL PETROL SAMPLES IN CULTURED HUMAN LYMPHOCYTES

Different concentrations of two petrol commercial samples, leaded and unleaded, were evaluated for genotoxicity in human peripheral blood lymphocyte cultures. Sister-chromatid exchanges (SCE) and micronuclei (MN) were scored as genetic endpoints to measure the genotoxicity of the samples in cultures...

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Detalhes bibliográficos
Autores: PITARQUE, Marià, CARBONELL, Elisabet, XAMENA, Noel, CREUS, Amadeu, MARCOS, Ricardo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:México
Recursos:UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO
Repositorio:Revista Internacional de Contaminación Ambiental
Idioma:español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/31355
Acesso em linha:https://www.revistascca.unam.mx/rica/index.php/rica/article/view/31355
Access Level:acceso abierto
Palavra-chave:petrol
human lymphocyte cultures
micronuclei
sister-chromatid exchanges
Descrição
Resumo:Different concentrations of two petrol commercial samples, leaded and unleaded, were evaluated for genotoxicity in human peripheral blood lymphocyte cultures. Sister-chromatid exchanges (SCE) and micronuclei (MN) were scored as genetic endpoints to measure the genotoxicity of the samples in cultures set up from three different healthy donors. The treatment of the cell culture was done employing different petrol concentrations (from 0.019 to 0.312 µl/ml). From our results we can conclude that both petrol samples induced a slight but concentration-dependent increase in the frequency of SCE, while no increase was detected in the MN frequency.