Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)

Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile s...

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Detalles Bibliográficos
Autores: Ma. del Carmen Flores-Miranda, Antonio Luna-González, Ángel I. Campa Córdova, Jesús A. Fierro-Coronado, Blanca O. Partida-Arangure, José Pintado, Héctor A. González-Ocampo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:México
Institución:Instituto Politécnico Nacional
Repositorio:Redalyc-IPN
OAI Identifier:oai:redalyc.org:44923872005
Acceso en línea:https://www.redalyc.org/articulo.oa?id=44923872005
Access Level:acceso abierto
Palabra clave:Biología
vibriosis
Vibrio sinaloensis
hemolytic activity
enzymatic activity
Litopenaeus vannamei
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spelling Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)Ma. del Carmen Flores-MirandaAntonio Luna-GonzálezÁngel I. Campa CórdovaJesús A. Fierro-CoronadoBlanca O. Partida-ArangureJosé PintadoHéctor A. González-OcampoBiologíavibriosisVibrio sinaloensishemolytic activityenzymatic activityLitopenaeus vannameiInfectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular identification. Three experimental infections were conducted in order to confirm the pathogenicity of selected bacterial strains VHPC18, VHPC23, VHPC24 and VIC30. In the third experimental challenge the LD50 was obtained, it lasted 10 days with 10 shrimp, weighing 6.9±1.1g, per tank. The treatments in triplicate were: (1) saline solution (control group); (2) 2×105CFU/shrimp; (3) 4×105CFU/shrimp; (4) 2×106CFU/shrimp; (5) 4×106CFU/shrimp, and (6) 8×106CFU/shrimp. In all challenges, water parameters measured during the experimental period remained within optimum ranges. Pathogenicity tests confirmed that the mixture of four vibrio isolates, identified as Vibrio sinaloensis, was virulent for L. vannamei. The LD50 value was 1.178×105CFU/g body weight. V. sinaloensis may act as opportunistic pathogens for cultured L. vannamei.Universidad de Costa Rica2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0034-7744https://www.redalyc.org/articulo.oa?id=44923872005Revista de Biología Tropical (Costa Rica) Num.2 Vol.60reponame:Redalyc-IPNinstname:Instituto Politécnico Nacionalinstacron:IPNenhttp://www.redalyc.org/revista.oa?id=449Revista de Biología Tropicalinfo:eu-repo/semantics/openAccessoai:redalyc.org:449238720052026-01-29T02:54:56Z
dc.title.none.fl_str_mv Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
title Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
spellingShingle Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
Ma. del Carmen Flores-Miranda
Biología
vibriosis
Vibrio sinaloensis
hemolytic activity
enzymatic activity
Litopenaeus vannamei
title_short Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
title_full Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
title_fullStr Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
title_full_unstemmed Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
title_sort Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
dc.creator.none.fl_str_mv Ma. del Carmen Flores-Miranda
Antonio Luna-González
Ángel I. Campa Córdova
Jesús A. Fierro-Coronado
Blanca O. Partida-Arangure
José Pintado
Héctor A. González-Ocampo
author Ma. del Carmen Flores-Miranda
author_facet Ma. del Carmen Flores-Miranda
Antonio Luna-González
Ángel I. Campa Córdova
Jesús A. Fierro-Coronado
Blanca O. Partida-Arangure
José Pintado
Héctor A. González-Ocampo
author_role author
author2 Antonio Luna-González
Ángel I. Campa Córdova
Jesús A. Fierro-Coronado
Blanca O. Partida-Arangure
José Pintado
Héctor A. González-Ocampo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
vibriosis
Vibrio sinaloensis
hemolytic activity
enzymatic activity
Litopenaeus vannamei
topic Biología
vibriosis
Vibrio sinaloensis
hemolytic activity
enzymatic activity
Litopenaeus vannamei
description Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular identification. Three experimental infections were conducted in order to confirm the pathogenicity of selected bacterial strains VHPC18, VHPC23, VHPC24 and VIC30. In the third experimental challenge the LD50 was obtained, it lasted 10 days with 10 shrimp, weighing 6.9±1.1g, per tank. The treatments in triplicate were: (1) saline solution (control group); (2) 2×105CFU/shrimp; (3) 4×105CFU/shrimp; (4) 2×106CFU/shrimp; (5) 4×106CFU/shrimp, and (6) 8×106CFU/shrimp. In all challenges, water parameters measured during the experimental period remained within optimum ranges. Pathogenicity tests confirmed that the mixture of four vibrio isolates, identified as Vibrio sinaloensis, was virulent for L. vannamei. The LD50 value was 1.178×105CFU/g body weight. V. sinaloensis may act as opportunistic pathogens for cultured L. vannamei.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0034-7744
https://www.redalyc.org/articulo.oa?id=44923872005
identifier_str_mv 0034-7744
url https://www.redalyc.org/articulo.oa?id=44923872005
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=449
dc.rights.none.fl_str_mv Revista de Biología Tropical
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Revista de Biología Tropical
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Revista de Biología Tropical (Costa Rica) Num.2 Vol.60
reponame:Redalyc-IPN
instname:Instituto Politécnico Nacional
instacron:IPN
instname_str Instituto Politécnico Nacional
instacron_str IPN
institution IPN
reponame_str Redalyc-IPN
collection Redalyc-IPN
repository.name.fl_str_mv
repository.mail.fl_str_mv
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