Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis

Background and Aims: Ulcerative colitis [UC] is a chronic inflammatory disease of the colon. Colonoscopy remains the gold standard for evaluating disease activity, as clinical symptoms are not sufficiently accurate. The aim of this study is to identify new accurate non-invasive biomarkers based on w...

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Autores: Planell Picola, Núria, Masamunt, Maria Carme, Franco Leal, Raquel, Rodríguez Rubio, Lorena, Esteller Viñal, Miriam, Lozano Salvatella, Juan José, Ramírez Morros, Anna, Setsuko Ayrizono, Maria de Lourdes, Coy, Claudio Saddy Rodrigues, Alfaro, Ignacio, Ordas, Ingrid, Visvanathan, Sudha, Ricart, Elena, Guardiola, Jordi, Panes, Julian, Salas Martínez, Azucena
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/221990
Acceso en línea:https://hdl.handle.net/2445/221990
Access Level:acceso abierto
Palabra clave:Colitis ulcerosa
Marcadors bioquímics
Ulcerative colitis
Biochemical markers
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spelling Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative ColitisPlanell Picola, NúriaMasamunt, Maria CarmeFranco Leal, RaquelRodríguez Rubio, LorenaEsteller Viñal, MiriamLozano Salvatella, Juan JoséRamírez Morros, AnnaSetsuko Ayrizono, Maria de LourdesCoy, Claudio Saddy RodriguesAlfaro, IgnacioOrdas, IngridVisvanathan, SudhaRicart, ElenaGuardiola, JordiPanes, JulianSalas Martínez, AzucenaColitis ulcerosaMarcadors bioquímicsUlcerative colitisBiochemical markersBackground and Aims: Ulcerative colitis [UC] is a chronic inflammatory disease of the colon. Colonoscopy remains the gold standard for evaluating disease activity, as clinical symptoms are not sufficiently accurate. The aim of this study is to identify new accurate non-invasive biomarkers based on whole-blood transcriptomics that can predict mucosal lesions and response to treatment in UC patients.Methods: Whole-blood samples were collected for a total of 152 UC patients at endoscopy. Blood RNA from 25 UC individuals and 20 controls was analysed using microarrays. Genes that correlated with endoscopic activity were validated using real-time polymerase chain reaction in an independent group of 111 UC patients, and a prediction model for mucosal lesions was evaluated. Responsiveness to treatment was assessed in a longitudinal cohort of 16 UC patients who started anti-tumour necrosis factor [TNF] therapy and were followed up for 14 weeks.Results: Microarray analysis identified 122 genes significantly altered in the blood of endoscopically active UC patients. A significant correlation with the degree of endoscopic activity was observed in several genes, including HP, CD177, GPR84, and S100A12. Using HP as a predictor of endoscopic disease activity, an accuracy of 67.3% was observed, compared with 52.4%, 45.2%, and 30.3% for C-reactive protein, erythrocyte sedimentation rate, and platelet count, respectively. Finally, at 14 weeks of treatment, response to anti-TNF therapy induced alterations in blood HP, CD177, GPR84, and S100A12 transcripts that correlated with changes in endoscopic activity.Conclusions: Transcriptional changes in UC patients are sensitive to endoscopic improvement and appear to be an effective tool to monitor patients over time.Elsevier2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/221990Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1093/ecco-jcc/jjx091Journal of Crohn's and Colitis, 2017, vol. 11, num.11, p. 1335-1346https://doi.org/10.1093/ecco-jcc/jjx091(c) Planell, Nuria et al., 2017info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2219902026-05-27T06:46:51Z
dc.title.none.fl_str_mv Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
title Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
spellingShingle Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
Planell Picola, Núria
Colitis ulcerosa
Marcadors bioquímics
Ulcerative colitis
Biochemical markers
title_short Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
title_full Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
title_fullStr Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
title_full_unstemmed Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
title_sort Usefulness of Transcriptional Blood Biomarkers as a Non-invasive Surrogate Marker of Mucosal Healing and Endoscopic Response in Ulcerative Colitis
dc.creator.none.fl_str_mv Planell Picola, Núria
Masamunt, Maria Carme
Franco Leal, Raquel
Rodríguez Rubio, Lorena
Esteller Viñal, Miriam
Lozano Salvatella, Juan José
Ramírez Morros, Anna
Setsuko Ayrizono, Maria de Lourdes
Coy, Claudio Saddy Rodrigues
Alfaro, Ignacio
Ordas, Ingrid
Visvanathan, Sudha
Ricart, Elena
Guardiola, Jordi
Panes, Julian
Salas Martínez, Azucena
author Planell Picola, Núria
author_facet Planell Picola, Núria
Masamunt, Maria Carme
Franco Leal, Raquel
Rodríguez Rubio, Lorena
Esteller Viñal, Miriam
Lozano Salvatella, Juan José
Ramírez Morros, Anna
Setsuko Ayrizono, Maria de Lourdes
Coy, Claudio Saddy Rodrigues
Alfaro, Ignacio
Ordas, Ingrid
Visvanathan, Sudha
Ricart, Elena
Guardiola, Jordi
Panes, Julian
Salas Martínez, Azucena
author_role author
author2 Masamunt, Maria Carme
Franco Leal, Raquel
Rodríguez Rubio, Lorena
Esteller Viñal, Miriam
Lozano Salvatella, Juan José
Ramírez Morros, Anna
Setsuko Ayrizono, Maria de Lourdes
Coy, Claudio Saddy Rodrigues
Alfaro, Ignacio
Ordas, Ingrid
Visvanathan, Sudha
Ricart, Elena
Guardiola, Jordi
Panes, Julian
Salas Martínez, Azucena
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Colitis ulcerosa
Marcadors bioquímics
Ulcerative colitis
Biochemical markers
topic Colitis ulcerosa
Marcadors bioquímics
Ulcerative colitis
Biochemical markers
description Background and Aims: Ulcerative colitis [UC] is a chronic inflammatory disease of the colon. Colonoscopy remains the gold standard for evaluating disease activity, as clinical symptoms are not sufficiently accurate. The aim of this study is to identify new accurate non-invasive biomarkers based on whole-blood transcriptomics that can predict mucosal lesions and response to treatment in UC patients.Methods: Whole-blood samples were collected for a total of 152 UC patients at endoscopy. Blood RNA from 25 UC individuals and 20 controls was analysed using microarrays. Genes that correlated with endoscopic activity were validated using real-time polymerase chain reaction in an independent group of 111 UC patients, and a prediction model for mucosal lesions was evaluated. Responsiveness to treatment was assessed in a longitudinal cohort of 16 UC patients who started anti-tumour necrosis factor [TNF] therapy and were followed up for 14 weeks.Results: Microarray analysis identified 122 genes significantly altered in the blood of endoscopically active UC patients. A significant correlation with the degree of endoscopic activity was observed in several genes, including HP, CD177, GPR84, and S100A12. Using HP as a predictor of endoscopic disease activity, an accuracy of 67.3% was observed, compared with 52.4%, 45.2%, and 30.3% for C-reactive protein, erythrocyte sedimentation rate, and platelet count, respectively. Finally, at 14 weeks of treatment, response to anti-TNF therapy induced alterations in blood HP, CD177, GPR84, and S100A12 transcripts that correlated with changes in endoscopic activity.Conclusions: Transcriptional changes in UC patients are sensitive to endoscopic improvement and appear to be an effective tool to monitor patients over time.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/221990
url https://hdl.handle.net/2445/221990
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1093/ecco-jcc/jjx091
Journal of Crohn's and Colitis, 2017, vol. 11, num.11, p. 1335-1346
https://doi.org/10.1093/ecco-jcc/jjx091
dc.rights.none.fl_str_mv (c) Planell, Nuria et al., 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Planell, Nuria et al., 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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