Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes

Ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) has been described in human non-pathological endometrium in both epithelial and stromal components without changes along the cycle. It was identified as a stromal marker of basalis. In the present study, we aimed to evaluate NTPDase2...

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Autores: Rodríguez-Martínez, Aitor, Trapero, Carla, Vidal, August, Piulats, Josep M., Gómez de Aranda Pulgarín, Inmaculada, Sévigny, Jean, Fernández Montolí, Ma. Eulalia, Ponce i Sebastià, Jordi, Matias-Guiu, Xavier, 1958-, Martín Satué, Mireia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/181136
Acceso en línea:https://hdl.handle.net/2445/181136
Access Level:acceso abierto
Palabra clave:Càncer d'endometri
Fenotip
Epiteli
Endometrial cancer
Phenotype
Epithelium
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spelling Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypesRodríguez-Martínez, AitorTrapero, CarlaVidal, AugustPiulats, Josep M.Gómez de Aranda Pulgarín, InmaculadaSévigny, JeanFernández Montolí, Ma. EulaliaPonce i Sebastià, JordiMatias-Guiu, Xavier, 1958-Martín Satué, MireiaCàncer d'endometriFenotipEpiteliEndometrial cancerPhenotypeEpitheliumEctonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) has been described in human non-pathological endometrium in both epithelial and stromal components without changes along the cycle. It was identified as a stromal marker of basalis. In the present study, we aimed to evaluate NTPDase2 distribution, using immunolabeling and in situ enzyme activity approaches, in endometrial carcinoma (EC) at different tumor grades. NTPDase2 was present in tumor epithelial EC cells, as in the non-pathological endometria, but the expression underwent changes in subcellular distribution and also tended to decrease with the tumor grade. In stroma, NTPDase2 was identified exclusively at the tumor-myometrial junction but this expression was lost in tumors of invasive phenotype. We have also identified in EC samples the presence of the perivascular population of endometrial mesenchymal stem cells (eMSCs) positive for sushi domain containing 2 (SUSD2) and for NTPDase2, already described in non-tumoral endometrium. Our results point to NTPDase2 as a histopathological marker of tumor invasion in EC, with diagnostic relevance especially in cases of EC coexisting with other endometrial disorders, such as adenomyosis, which occasionally hampers the assessment of tumor invasion parameters.MDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/181136Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/jpm11050331Journal of Personalized Medicine, 2021, vol. 11, num. 5, p. 331https://doi.org/10.3390/jpm11050331cc-by (c) Rodríguez-Martínez, Aitor et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1811362026-05-27T06:46:51Z
dc.title.none.fl_str_mv Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
title Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
spellingShingle Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
Rodríguez-Martínez, Aitor
Càncer d'endometri
Fenotip
Epiteli
Endometrial cancer
Phenotype
Epithelium
title_short Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
title_full Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
title_fullStr Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
title_full_unstemmed Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
title_sort Characterization of the endometrial MSC marker ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) in low- and high-grade endometrial carcinomas: loss of stromal expression in the invasive phenotypes
dc.creator.none.fl_str_mv Rodríguez-Martínez, Aitor
Trapero, Carla
Vidal, August
Piulats, Josep M.
Gómez de Aranda Pulgarín, Inmaculada
Sévigny, Jean
Fernández Montolí, Ma. Eulalia
Ponce i Sebastià, Jordi
Matias-Guiu, Xavier, 1958-
Martín Satué, Mireia
author Rodríguez-Martínez, Aitor
author_facet Rodríguez-Martínez, Aitor
Trapero, Carla
Vidal, August
Piulats, Josep M.
Gómez de Aranda Pulgarín, Inmaculada
Sévigny, Jean
Fernández Montolí, Ma. Eulalia
Ponce i Sebastià, Jordi
Matias-Guiu, Xavier, 1958-
Martín Satué, Mireia
author_role author
author2 Trapero, Carla
Vidal, August
Piulats, Josep M.
Gómez de Aranda Pulgarín, Inmaculada
Sévigny, Jean
Fernández Montolí, Ma. Eulalia
Ponce i Sebastià, Jordi
Matias-Guiu, Xavier, 1958-
Martín Satué, Mireia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer d'endometri
Fenotip
Epiteli
Endometrial cancer
Phenotype
Epithelium
topic Càncer d'endometri
Fenotip
Epiteli
Endometrial cancer
Phenotype
Epithelium
description Ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2/CD39L1) has been described in human non-pathological endometrium in both epithelial and stromal components without changes along the cycle. It was identified as a stromal marker of basalis. In the present study, we aimed to evaluate NTPDase2 distribution, using immunolabeling and in situ enzyme activity approaches, in endometrial carcinoma (EC) at different tumor grades. NTPDase2 was present in tumor epithelial EC cells, as in the non-pathological endometria, but the expression underwent changes in subcellular distribution and also tended to decrease with the tumor grade. In stroma, NTPDase2 was identified exclusively at the tumor-myometrial junction but this expression was lost in tumors of invasive phenotype. We have also identified in EC samples the presence of the perivascular population of endometrial mesenchymal stem cells (eMSCs) positive for sushi domain containing 2 (SUSD2) and for NTPDase2, already described in non-tumoral endometrium. Our results point to NTPDase2 as a histopathological marker of tumor invasion in EC, with diagnostic relevance especially in cases of EC coexisting with other endometrial disorders, such as adenomyosis, which occasionally hampers the assessment of tumor invasion parameters.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/181136
url https://hdl.handle.net/2445/181136
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/jpm11050331
Journal of Personalized Medicine, 2021, vol. 11, num. 5, p. 331
https://doi.org/10.3390/jpm11050331
dc.rights.none.fl_str_mv cc-by (c) Rodríguez-Martínez, Aitor et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Rodríguez-Martínez, Aitor et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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