Coupled C, H, N, S and Fe biogeochemical cycles operating in the continental deep subsurface of the Iberian Pyrite Belt

Microbial activity is a major contributor to the biogeochemical cycles that make up the life support system of planet Earth. A 613 m deep geomicrobiological perforation and a systematic multi-analytical characterization revealed an unexpected diversity associated with the rock matrix microbiome that...

ver descrição completa

Detalhes bibliográficos
Autores: Amils Pibernat, Ricardo, Escudero, Cristina, Oggerin, Monike, Puente Sanchez, Fernando, Arce Rodríguez, Alejandro, Fernandez Remolar, David, Rodríguez Salas, Nuria, García Villadangos, Miriam, Sanz Martín, José Luis, Briones, Carlos, Sánchez Román, Mónica, Gomez, Felipe, Leandro, Tania, Moreno Paz, Mercedes, Prieto Ballesteros, Olga, Molina, Antonio, Tornos, Fernando, Sanchez Andrea, Irene, Timmis, Kenneth, Pieper, Dietmar H., Parro, Victor
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/705734
Acesso em linha:http://hdl.handle.net/10486/705734
https://dx.doi.org/10.1111/1462-2920.16291
Access Level:acceso abierto
Palavra-chave:Biología y Biomedicina / Biología
Descrição
Resumo:Microbial activity is a major contributor to the biogeochemical cycles that make up the life support system of planet Earth. A 613 m deep geomicrobiological perforation and a systematic multi-analytical characterization revealed an unexpected diversity associated with the rock matrix microbiome that operates in the subsurface of the Iberian Pyrite Belt (IPB). Members of 1 class and 16 genera were deemed the most representative microorganisms of the IPB deep subsurface and selected for a deeper analysis. The use of fluorescence in situ hybridization allowed not only the identification of microorganisms but also the detection of novel activities in the subsurface such as anaerobic ammonium oxidation (ANAMMOX) and anaerobic methane oxidation, the co-occurrence of microorganisms able to maintain complementary metabolic activities and the existence of biofilms. The use of enrichment cultures sensed the presence of five different complementary metabolic activities along the length of the borehole and isolated 29 bacterial species. Genomic analysis of nine isolates identified the genes involved in the complete operation of the light-independent coupled C, H, N, S and Fe biogeochemical cycles. This study revealed the importance of nitrate reduction microorganisms in the oxidation of iron in the anoxic conditions existing in the subsurface of the IPB

Registos relacionados