A benchmark of transposon insertion detection tools using real data

[Background] Transposable elements (TEs) are an important source of genomic variability in eukaryotic genomes. Their activity impacts genome architecture and gene expression and can lead to drastic phenotypic changes. Therefore, identifying TE polymorphisms is key to better understand the link betwe...

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Detalles Bibliográficos
Autores: Vendrell-Mir, Pol, Barteri, Fabio, Merenciano, Miriam, González Pérez, Josefa, Casacuberta, Josep M., Castanera, Raúl
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/205901
Acceso en línea:http://hdl.handle.net/10261/205901
Access Level:acceso abierto
Palabra clave:Benchmarks
Transposable elements
Polymorphism
Transposon insertion
Resequencing
Descripción
Sumario:[Background] Transposable elements (TEs) are an important source of genomic variability in eukaryotic genomes. Their activity impacts genome architecture and gene expression and can lead to drastic phenotypic changes. Therefore, identifying TE polymorphisms is key to better understand the link between genotype and phenotype. However, most genotype-to-phenotype analyses have concentrated on single nucleotide polymorphisms as they are easier to reliable detect using short-read data. Many bioinformatic tools have been developed to identify transposon insertions from resequencing data using short reads. Nevertheless, the performance of most of these tools has been tested using simulated insertions, which do not accurately reproduce the complexity of natural insertions.