High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization

In this study, we examine the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue. We also compare the performance of desorption electrospray ionization (DESI) with and without the flow focusing setup. The main potential advanta...

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Detalhes bibliográficos
Autores: Wu, Vincen, Tillner, Jocelyn, Jones, Emrys, McKenzie, James S, Gurung, Dipa, Mroz, Anna, Poynter, Liam, Simon, Daniel, Grau, Cristina, Altafaj, Xavier, Dumas, Marc-Emmanuel, Gilmore, Ian, Bunch, Josephine, Takats, Zoltan
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/194393
Acesso em linha:https://hdl.handle.net/2445/194393
Access Level:Acceso aberto
Palavra-chave:Cervell
Diagnòstic per la imatge
Espectrometria de masses
Espectrometria de masses amb ionització per electroesprai
Ratolins (Animals de laboratori)
Brain
Diagnostic imaging
Mass spectrometry
Electrospray ionization mass spectrometry
Mice (Laboratory animals)
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oai_identifier_str oai:recercat.cat:2445/194393
network_acronym_str ES
network_name_str España
repository_id_str
spelling High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionizationWu, VincenTillner, JocelynJones, EmrysMcKenzie, James SGurung, DipaMroz, AnnaPoynter, LiamSimon, DanielGrau, CristinaAltafaj, XavierDumas, Marc-EmmanuelGilmore, IanBunch, JosephineTakats, ZoltanCervellDiagnòstic per la imatgeEspectrometria de massesEspectrometria de masses amb ionització per electroespraiRatolins (Animals de laboratori)BrainDiagnostic imagingMass spectrometryElectrospray ionization mass spectrometryMice (Laboratory animals)In this study, we examine the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue. We also compare the performance of desorption electrospray ionization (DESI) with and without the flow focusing setup. The main potential advantages of applying the flow focusing mechanism in DESI is its rotationally symmetric electrospray jet, higher intensity, more controllable parameters, and better portability due to the robustness of the sprayer. The parameters for DEFFI have therefore been thoroughly optimized, primarily for spatial resolution but also for intensity. Once the parameters have been optimized, DEFFI produces similar images to the existing DESI. MS images for mouse brain samples, acquired at a nominal pixel size of 50 μm, are comparable for both DESI setups, albeit the new sprayer design yields better sensitivity. Furthermore, the two methods are compared with regard to spectral intensity as well as the area of the desorbed crater on rhodamine-coated slides. Overall, the implementation of a flow focusing mechanism in DESI is shown to be highly suitable for imaging biological tissue and has potential to overcome some of the shortcomings experienced with the current geometrical design of DESI.American Chemical Society202320222023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/194393Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1021/acs.analchem.2c00345Analytical Chemistry, 2022, vol. 94, num. 28, p. 10035-10044https://doi.org/10.1021/acs.analchem.2c00345cc-by (c) Wu, Vincen et al., 2022http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1943932026-05-29T05:05:01Z
dc.title.none.fl_str_mv High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
title High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
spellingShingle High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
Wu, Vincen
Cervell
Diagnòstic per la imatge
Espectrometria de masses
Espectrometria de masses amb ionització per electroesprai
Ratolins (Animals de laboratori)
Brain
Diagnostic imaging
Mass spectrometry
Electrospray ionization mass spectrometry
Mice (Laboratory animals)
title_short High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
title_full High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
title_fullStr High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
title_full_unstemmed High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
title_sort High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
dc.creator.none.fl_str_mv Wu, Vincen
Tillner, Jocelyn
Jones, Emrys
McKenzie, James S
Gurung, Dipa
Mroz, Anna
Poynter, Liam
Simon, Daniel
Grau, Cristina
Altafaj, Xavier
Dumas, Marc-Emmanuel
Gilmore, Ian
Bunch, Josephine
Takats, Zoltan
author Wu, Vincen
author_facet Wu, Vincen
Tillner, Jocelyn
Jones, Emrys
McKenzie, James S
Gurung, Dipa
Mroz, Anna
Poynter, Liam
Simon, Daniel
Grau, Cristina
Altafaj, Xavier
Dumas, Marc-Emmanuel
Gilmore, Ian
Bunch, Josephine
Takats, Zoltan
author_role author
author2 Tillner, Jocelyn
Jones, Emrys
McKenzie, James S
Gurung, Dipa
Mroz, Anna
Poynter, Liam
Simon, Daniel
Grau, Cristina
Altafaj, Xavier
Dumas, Marc-Emmanuel
Gilmore, Ian
Bunch, Josephine
Takats, Zoltan
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cervell
Diagnòstic per la imatge
Espectrometria de masses
Espectrometria de masses amb ionització per electroesprai
Ratolins (Animals de laboratori)
Brain
Diagnostic imaging
Mass spectrometry
Electrospray ionization mass spectrometry
Mice (Laboratory animals)
topic Cervell
Diagnòstic per la imatge
Espectrometria de masses
Espectrometria de masses amb ionització per electroesprai
Ratolins (Animals de laboratori)
Brain
Diagnostic imaging
Mass spectrometry
Electrospray ionization mass spectrometry
Mice (Laboratory animals)
description In this study, we examine the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue. We also compare the performance of desorption electrospray ionization (DESI) with and without the flow focusing setup. The main potential advantages of applying the flow focusing mechanism in DESI is its rotationally symmetric electrospray jet, higher intensity, more controllable parameters, and better portability due to the robustness of the sprayer. The parameters for DEFFI have therefore been thoroughly optimized, primarily for spatial resolution but also for intensity. Once the parameters have been optimized, DEFFI produces similar images to the existing DESI. MS images for mouse brain samples, acquired at a nominal pixel size of 50 μm, are comparable for both DESI setups, albeit the new sprayer design yields better sensitivity. Furthermore, the two methods are compared with regard to spectral intensity as well as the area of the desorbed crater on rhodamine-coated slides. Overall, the implementation of a flow focusing mechanism in DESI is shown to be highly suitable for imaging biological tissue and has potential to overcome some of the shortcomings experienced with the current geometrical design of DESI.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/194393
url https://hdl.handle.net/2445/194393
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1021/acs.analchem.2c00345
Analytical Chemistry, 2022, vol. 94, num. 28, p. 10035-10044
https://doi.org/10.1021/acs.analchem.2c00345
dc.rights.none.fl_str_mv cc-by (c) Wu, Vincen et al., 2022
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Wu, Vincen et al., 2022
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425812526071808
score 15.81155

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