Transcription factor-based biosensors enlightened by the analyte

Whole cell biosensors (WCBs) have multiple applications for environmental monitoring, detecting a wide range of pollutants. WCBs depend critically on the sensitivity and specificity of the transcription factor (TF) used to detect the analyte. We describe the mechanism of regulation and the structura...

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Detalhes bibliográficos
Autores: Fernández López, Raúl, Ruiz González, Raúl, Cruz, Fernando de la|||0000-0003-4758-6857, Moncalián Montes, Gabriel|||0000-0002-3007-6490
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/7098
Acesso em linha:http://hdl.handle.net/10902/7098
Access Level:acceso abierto
Palavra-chave:Analyte
Aromatic compounds
Biosensors
Effector
Metal
Transcription factor
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spelling Transcription factor-based biosensors enlightened by the analyteFernández López, RaúlRuiz González, RaúlCruz, Fernando de la|||0000-0003-4758-6857Moncalián Montes, Gabriel|||0000-0002-3007-6490AnalyteAromatic compoundsBiosensorsEffectorMetalTranscription factorWhole cell biosensors (WCBs) have multiple applications for environmental monitoring, detecting a wide range of pollutants. WCBs depend critically on the sensitivity and specificity of the transcription factor (TF) used to detect the analyte. We describe the mechanism of regulation and the structural and biochemical properties of TF families that are used, or could be used, for the development of environmental WCBs. Focusing on the chemical nature of the analyte, we review TFs that respond to aromatic compounds (XylS-AraC, XylR-NtrC, and LysR), metal ions (MerR, ArsR, DtxR, Fur, and NikR) or antibiotics (TetR and MarR). Analyzing the structural domains involved in DNA recognition, we highlight the similitudes in the DNA binding domains (DBDs) of these TF families. Opposite to DBDs, the wide range of analytes detected by TFs results in a diversity of structures at the effector binding domain. The modular architecture of TFs opens the possibility of engineering TFs with hybrid DNA and effector specificities. Yet, the lack of a crisp correlation between structural domains and specific functions makes this a challenging task.We are grateful to Spanish Ministry of Economy for Grant BIO2010-14809 to GM. Work in FdlC laboratory was financed by the Spanish Ministry of Economy and Competitivity (BFU2011-26608) and by the European Seventh Framework Program [projects 612146/FP7-ICT-2013-10 (PLASWIRES), 289326/KBBE-2011-5 (ST-FLOW) and 282004/FP7-HEALTH-2011-2.3.1-2 (EvoTAR)].FrontiersUniversidad de Cantabria20152015-07-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/7098Frontiers in Microbiology. 2015, 6, 648reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)InglésengEuropean Commission http://dx.doi.org/10.13039/501100000780 Framework Programme Seven 282004open accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/70982026-06-02T12:39:31Z
dc.title.none.fl_str_mv Transcription factor-based biosensors enlightened by the analyte
title Transcription factor-based biosensors enlightened by the analyte
spellingShingle Transcription factor-based biosensors enlightened by the analyte
Fernández López, Raúl
Analyte
Aromatic compounds
Biosensors
Effector
Metal
Transcription factor
title_short Transcription factor-based biosensors enlightened by the analyte
title_full Transcription factor-based biosensors enlightened by the analyte
title_fullStr Transcription factor-based biosensors enlightened by the analyte
title_full_unstemmed Transcription factor-based biosensors enlightened by the analyte
title_sort Transcription factor-based biosensors enlightened by the analyte
dc.creator.none.fl_str_mv Fernández López, Raúl
Ruiz González, Raúl
Cruz, Fernando de la|||0000-0003-4758-6857
Moncalián Montes, Gabriel|||0000-0002-3007-6490
author Fernández López, Raúl
author_facet Fernández López, Raúl
Ruiz González, Raúl
Cruz, Fernando de la|||0000-0003-4758-6857
Moncalián Montes, Gabriel|||0000-0002-3007-6490
author_role author
author2 Ruiz González, Raúl
Cruz, Fernando de la|||0000-0003-4758-6857
Moncalián Montes, Gabriel|||0000-0002-3007-6490
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Analyte
Aromatic compounds
Biosensors
Effector
Metal
Transcription factor
topic Analyte
Aromatic compounds
Biosensors
Effector
Metal
Transcription factor
description Whole cell biosensors (WCBs) have multiple applications for environmental monitoring, detecting a wide range of pollutants. WCBs depend critically on the sensitivity and specificity of the transcription factor (TF) used to detect the analyte. We describe the mechanism of regulation and the structural and biochemical properties of TF families that are used, or could be used, for the development of environmental WCBs. Focusing on the chemical nature of the analyte, we review TFs that respond to aromatic compounds (XylS-AraC, XylR-NtrC, and LysR), metal ions (MerR, ArsR, DtxR, Fur, and NikR) or antibiotics (TetR and MarR). Analyzing the structural domains involved in DNA recognition, we highlight the similitudes in the DNA binding domains (DBDs) of these TF families. Opposite to DBDs, the wide range of analytes detected by TFs results in a diversity of structures at the effector binding domain. The modular architecture of TFs opens the possibility of engineering TFs with hybrid DNA and effector specificities. Yet, the lack of a crisp correlation between structural domains and specific functions makes this a challenging task.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-07-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10902/7098
url http://hdl.handle.net/10902/7098
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://dx.doi.org/10.13039/501100000780 Framework Programme Seven 282004
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
http://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv Frontiers in Microbiology. 2015, 6, 648
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425797448597504
score 15,300719

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