Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18
Haematopoietic malignancies are frequently characterized by karyotypic abnormalities. The development of targeted drugs has been pioneered with compounds against gene products of fusion genes caused by chromosomal translocations. While polysomies are equally frequent as translocations, for many of t...
| Autores: | , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:270563 |
| Acceso en línea: | https://ddd.uab.cat/record/270563 https://dx.doi.org/urn:doi:10.1038/s41598-021-00623-w |
| Access Level: | acceso abierto |
| Palabra clave: | Biomarkers, Tumor Cell Line, Tumor Chromosomes, Human, Pair 18 Chromosomes, Human, Pair 7 Chromosomes, Human, Pair 8 Humans Leukemia, Myeloid, Acute Tetrasomy Trisomy |
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Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18Maher, Michael|||0000-0002-4956-7185Diesch, Jeannine|||0000-0002-4667-3468Le Pannérer, Marguerite Marie|||0000-0002-5779-8805Cabezón, Marta|||0000-0001-5122-7481Mallo, Maria del Mar|||0000-0001-7741-498XVergara, SaraMéndez López, AleixMesa Tudel, Alba|||0000-0002-8377-7573Sole, F.|||0000-0002-3251-2161Sorigue, Marc|||0000-0002-0587-591XZamora, Lurdes|||0000-0003-1713-7110Granada, Isabel|||0000-0002-4275-0104Buschbeck, Marcus|||0000-0002-3218-4567Biomarkers, TumorCell Line, TumorChromosomes, Human, Pair 18Chromosomes, Human, Pair 7Chromosomes, Human, Pair 8HumansLeukemia, Myeloid, AcuteTetrasomyTrisomyHaematopoietic malignancies are frequently characterized by karyotypic abnormalities. The development of targeted drugs has been pioneered with compounds against gene products of fusion genes caused by chromosomal translocations. While polysomies are equally frequent as translocations, for many of them we are lacking therapeutic approaches aimed at synthetic lethality. Here, we report two new cell lines, named MBU-7 and MBU-8, that differ in complete trisomy of chromosome18, a partial trisomy of chromosome 7 and a tetrasomy of the p-arm of chromosome 8, but otherwise share the same mutational pattern and complex karyotype. Both cell lines are divergent clones of U-937 cells and have the morphology and immunoprofile of monocytic cells. The distinct karyotypic differences between MBU-7 and MBU-8 are associated with a difference in the specific response to nucleoside analogues. Taken together, we propose the MBU-7 and MBU-8 cell lines described here as suitable in vitro models for screening and testing vulnerabilities that are associated with the disease-relevant polysomies of chromosome 7, 8 and 18.Universitat Autònoma de Barcelona 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/270563https://dx.doi.org/urn:doi:10.1038/s41598-021-00623-wreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengFundació la Marató de TV3 https://doi.org/10.13039/100008666 257/C/2019Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CD17/00084Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PIE16/00011Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-305Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-094005-B-I00open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2705632026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| title |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| spellingShingle |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 Maher, Michael|||0000-0002-4956-7185 Biomarkers, Tumor Cell Line, Tumor Chromosomes, Human, Pair 18 Chromosomes, Human, Pair 7 Chromosomes, Human, Pair 8 Humans Leukemia, Myeloid, Acute Tetrasomy Trisomy |
| title_short |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| title_full |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| title_fullStr |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| title_full_unstemmed |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| title_sort |
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| dc.creator.none.fl_str_mv |
Maher, Michael|||0000-0002-4956-7185 Diesch, Jeannine|||0000-0002-4667-3468 Le Pannérer, Marguerite Marie|||0000-0002-5779-8805 Cabezón, Marta|||0000-0001-5122-7481 Mallo, Maria del Mar|||0000-0001-7741-498X Vergara, Sara Méndez López, Aleix Mesa Tudel, Alba|||0000-0002-8377-7573 Sole, F.|||0000-0002-3251-2161 Sorigue, Marc|||0000-0002-0587-591X Zamora, Lurdes|||0000-0003-1713-7110 Granada, Isabel|||0000-0002-4275-0104 Buschbeck, Marcus|||0000-0002-3218-4567 |
| author |
Maher, Michael|||0000-0002-4956-7185 |
| author_facet |
Maher, Michael|||0000-0002-4956-7185 Diesch, Jeannine|||0000-0002-4667-3468 Le Pannérer, Marguerite Marie|||0000-0002-5779-8805 Cabezón, Marta|||0000-0001-5122-7481 Mallo, Maria del Mar|||0000-0001-7741-498X Vergara, Sara Méndez López, Aleix Mesa Tudel, Alba|||0000-0002-8377-7573 Sole, F.|||0000-0002-3251-2161 Sorigue, Marc|||0000-0002-0587-591X Zamora, Lurdes|||0000-0003-1713-7110 Granada, Isabel|||0000-0002-4275-0104 Buschbeck, Marcus|||0000-0002-3218-4567 |
| author_role |
author |
| author2 |
Diesch, Jeannine|||0000-0002-4667-3468 Le Pannérer, Marguerite Marie|||0000-0002-5779-8805 Cabezón, Marta|||0000-0001-5122-7481 Mallo, Maria del Mar|||0000-0001-7741-498X Vergara, Sara Méndez López, Aleix Mesa Tudel, Alba|||0000-0002-8377-7573 Sole, F.|||0000-0002-3251-2161 Sorigue, Marc|||0000-0002-0587-591X Zamora, Lurdes|||0000-0003-1713-7110 Granada, Isabel|||0000-0002-4275-0104 Buschbeck, Marcus|||0000-0002-3218-4567 |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Biomarkers, Tumor Cell Line, Tumor Chromosomes, Human, Pair 18 Chromosomes, Human, Pair 7 Chromosomes, Human, Pair 8 Humans Leukemia, Myeloid, Acute Tetrasomy Trisomy |
| topic |
Biomarkers, Tumor Cell Line, Tumor Chromosomes, Human, Pair 18 Chromosomes, Human, Pair 7 Chromosomes, Human, Pair 8 Humans Leukemia, Myeloid, Acute Tetrasomy Trisomy |
| description |
Haematopoietic malignancies are frequently characterized by karyotypic abnormalities. The development of targeted drugs has been pioneered with compounds against gene products of fusion genes caused by chromosomal translocations. While polysomies are equally frequent as translocations, for many of them we are lacking therapeutic approaches aimed at synthetic lethality. Here, we report two new cell lines, named MBU-7 and MBU-8, that differ in complete trisomy of chromosome18, a partial trisomy of chromosome 7 and a tetrasomy of the p-arm of chromosome 8, but otherwise share the same mutational pattern and complex karyotype. Both cell lines are divergent clones of U-937 cells and have the morphology and immunoprofile of monocytic cells. The distinct karyotypic differences between MBU-7 and MBU-8 are associated with a difference in the specific response to nucleoside analogues. Taken together, we propose the MBU-7 and MBU-8 cell lines described here as suitable in vitro models for screening and testing vulnerabilities that are associated with the disease-relevant polysomies of chromosome 7, 8 and 18. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2 2021-01-01 2021 2021-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/270563 https://dx.doi.org/urn:doi:10.1038/s41598-021-00623-w |
| url |
https://ddd.uab.cat/record/270563 https://dx.doi.org/urn:doi:10.1038/s41598-021-00623-w |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Fundació la Marató de TV3 https://doi.org/10.13039/100008666 257/C/2019 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CD17/00084 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PIE16/00011 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-305 Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-094005-B-I00 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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Dipòsit Digital de Documents de la UAB |
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