Distinct responses of active and total bacterial communities to inorganic fertilization in a 30-year experimental site
Soil microbial communities play a vital role in mediating nutrient turnover, thus enhancing growth and development of plants. Understanding the dynamics of microorganisms in soils can provide insight into the influence of fertilization practices on improving soil fertility and plant growth in agricu...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:299900 |
| Acceso en línea: | https://ddd.uab.cat/record/299900 https://dx.doi.org/urn:doi:10.1016/j.apsoil.2022.104584 |
| Access Level: | acceso abierto |
| Palabra clave: | Inorganic fertilization Total microbes Active microbes RNA Bacterial community |
| Sumario: | Soil microbial communities play a vital role in mediating nutrient turnover, thus enhancing growth and development of plants. Understanding the dynamics of microorganisms in soils can provide insight into the influence of fertilization practices on improving soil fertility and plant growth in agricultural ecosystems. In this study, we compared the abundances and compositions of total (DNA-based, 16S rRNA gene) and active (RNA-based, 16S rRNA) bacterial communities at a 30-year experimental site in different inorganic fertilization treatments with different key elements (nitrogen, phosphorus, and potassium). The inorganic fertilizer amendments did not affect the abundance of total bacteria but significantly affected the abundance of active bacteria due to changes in microbial biomass carbon and NH4+-N contents. Cyanobacteria and Proteobacteria, especially for some dominant orders (e.g. Nostocales, Pseudanabaenales and Nitrosomonadales) were the dominant phyla in the active microbial community and differed proportionally in nitrogen and phosphorus fertilized soil. Soil N speciation (e.g. total N, NH4+-N and NO3--N) were the main determinants controlling the Cyanobacteria and Proteobacteria communities. Our results indicated that the unbalanced fertilization could reduce the abundance of active bacteria and significantly changed the dominant phyla compared with balanced fertilization. These findings provided an insight of composition and ratio of nutrient elements including nitrogen, phosphorus and potassium for managing future fertilization regimes in agricultural ecosystem. |
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