Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia

This study examines the influence of intermittent exposure to cold, hypobaric hypoxia, and their combination, in gut microbiota and their metabolites in vivo, and explores their effects on the physiology of the host. Sprague-Dawley rats were exposed to cold (4ºC), hypobaric hypoxia (462 torr), or bo...

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Autores: Ramos Romero, Sara, Santocildes Martinez, Garoa, Piñol Piñol, David, Rosés, Carles, Pagés, Teresa, Hereu, Mercè, Amézqueta, Susana, Torrella Guio, Joan Ramon, Torres Simón, Josep Lluís, Viscor Carrasco, Ginés
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2020
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/174277
Acesso em linha:https://hdl.handle.net/2445/174277
Access Level:Acceso aberto
Palavra-chave:Microbiota intestinal
Anoxèmia
Gastrointestinal microbiome
Anoxemia
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spelling Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxiaRamos Romero, SaraSantocildes Martinez, GaroaPiñol Piñol, DavidRosés, CarlesPagés, TeresaHereu, MercèAmézqueta, SusanaTorrella Guio, Joan RamonTorres Simón, Josep LluísViscor Carrasco, GinésMicrobiota intestinalAnoxèmiaGastrointestinal microbiomeAnoxemiaThis study examines the influence of intermittent exposure to cold, hypobaric hypoxia, and their combination, in gut microbiota and their metabolites in vivo, and explores their effects on the physiology of the host. Sprague-Dawley rats were exposed to cold (4ºC), hypobaric hypoxia (462 torr), or both simultaneously, 4 h/day for 21 days. Biometrical and hematological parameters were monitored. Gut bacterial subgroups were evaluated by qRT-PCR and short-chain fatty acids were determined by gas chromatography in caecum and feces. Cold increased brown adipose tissue, Clostridiales subpopulation and the concentration of butyric and isovaleric acids in caecum. Hypobaric hypoxia increased hemoglobin, red and white cell counts and Enterobacteriales, and reduced body and adipose tissues weights and Lactobacilliales. Cold plus hypobaric hypoxia counteracted the hypoxia-induced weight loss as well as the increase in white blood cells, while reducing the Bacteroidetes:Firmicutes ratio and normalizing the populations of Enterobacteriales and Lactobacilliales. In conclusion, intermittent cold and hypobaric hypoxia exposures by themselves modified some of the main physiological variables in vivo , while their combination kept the rats nearer to their basal status. The reduction of the Bacteroidetes:Firmicutes ratio and balanced populations of Enterobacteriales and Lactobacilliales in the gut may contribute to this effect.Public Library of Science (PLoS)2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/174277Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0240686PLoS One, 2020, vol. 15, num. 11, p. e0240686https://doi.org/10.1371/journal.pone.0240686cc-by (c) Ramos Romero, Sara et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1742772026-05-27T06:46:51Z
dc.title.none.fl_str_mv Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
title Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
spellingShingle Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
Ramos Romero, Sara
Microbiota intestinal
Anoxèmia
Gastrointestinal microbiome
Anoxemia
title_short Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
title_full Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
title_fullStr Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
title_full_unstemmed Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
title_sort Implication of gut microbiota in the physiology of rats intermittently exposed to cold and hypobaric hypoxia
dc.creator.none.fl_str_mv Ramos Romero, Sara
Santocildes Martinez, Garoa
Piñol Piñol, David
Rosés, Carles
Pagés, Teresa
Hereu, Mercè
Amézqueta, Susana
Torrella Guio, Joan Ramon
Torres Simón, Josep Lluís
Viscor Carrasco, Ginés
author Ramos Romero, Sara
author_facet Ramos Romero, Sara
Santocildes Martinez, Garoa
Piñol Piñol, David
Rosés, Carles
Pagés, Teresa
Hereu, Mercè
Amézqueta, Susana
Torrella Guio, Joan Ramon
Torres Simón, Josep Lluís
Viscor Carrasco, Ginés
author_role author
author2 Santocildes Martinez, Garoa
Piñol Piñol, David
Rosés, Carles
Pagés, Teresa
Hereu, Mercè
Amézqueta, Susana
Torrella Guio, Joan Ramon
Torres Simón, Josep Lluís
Viscor Carrasco, Ginés
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Microbiota intestinal
Anoxèmia
Gastrointestinal microbiome
Anoxemia
topic Microbiota intestinal
Anoxèmia
Gastrointestinal microbiome
Anoxemia
description This study examines the influence of intermittent exposure to cold, hypobaric hypoxia, and their combination, in gut microbiota and their metabolites in vivo, and explores their effects on the physiology of the host. Sprague-Dawley rats were exposed to cold (4ºC), hypobaric hypoxia (462 torr), or both simultaneously, 4 h/day for 21 days. Biometrical and hematological parameters were monitored. Gut bacterial subgroups were evaluated by qRT-PCR and short-chain fatty acids were determined by gas chromatography in caecum and feces. Cold increased brown adipose tissue, Clostridiales subpopulation and the concentration of butyric and isovaleric acids in caecum. Hypobaric hypoxia increased hemoglobin, red and white cell counts and Enterobacteriales, and reduced body and adipose tissues weights and Lactobacilliales. Cold plus hypobaric hypoxia counteracted the hypoxia-induced weight loss as well as the increase in white blood cells, while reducing the Bacteroidetes:Firmicutes ratio and normalizing the populations of Enterobacteriales and Lactobacilliales. In conclusion, intermittent cold and hypobaric hypoxia exposures by themselves modified some of the main physiological variables in vivo , while their combination kept the rats nearer to their basal status. The reduction of the Bacteroidetes:Firmicutes ratio and balanced populations of Enterobacteriales and Lactobacilliales in the gut may contribute to this effect.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/174277
url https://hdl.handle.net/2445/174277
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0240686
PLoS One, 2020, vol. 15, num. 11, p. e0240686
https://doi.org/10.1371/journal.pone.0240686
dc.rights.none.fl_str_mv cc-by (c) Ramos Romero, Sara et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Ramos Romero, Sara et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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