Development of an antimicrobial bioactive paper made from bacterial cellulose

Bacterial cellulose (BC) has emerged as an attractive adsorptive material for antimicrobial agents due to its fine network structure, its large surface area, and its high porosity. In the present study, BC paper was first produced and then lysozymewas immobilized onto it by physical adsorption, obta...

Descripción completa

Detalles Bibliográficos
Autores: Buruaga Ramiro, Carolina, Valenzuela Mayorga, Susana Valeria, Valls Vidal, Cristina, Roncero, M. Blanca, Pastor Blasco, Francisco I. Javier, Díaz Lucea, Pilar, Martínez Martínez, Josefina
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/164756
Acceso en línea:https://hdl.handle.net/2445/164756
Access Level:acceso abierto
Palabra clave:Cel·lulosa
Microbiologia
Fabricació del paper
Cellulose
Microbiology
Papermaking
id ES_ff560f7fd5ddcdcc7e64c96b5cbfe208
oai_identifier_str oai:recercat.cat:2445/164756
network_acronym_str ES
network_name_str España
repository_id_str
spelling Development of an antimicrobial bioactive paper made from bacterial celluloseBuruaga Ramiro, CarolinaValenzuela Mayorga, Susana ValeriaValls Vidal, CristinaRoncero, M. BlancaPastor Blasco, Francisco I. JavierDíaz Lucea, PilarMartínez Martínez, JosefinaCel·lulosaMicrobiologiaFabricació del paperCelluloseMicrobiologyPapermakingBacterial cellulose (BC) has emerged as an attractive adsorptive material for antimicrobial agents due to its fine network structure, its large surface area, and its high porosity. In the present study, BC paper was first produced and then lysozymewas immobilized onto it by physical adsorption, obtaining a composite of lysozyme-BC paper. The morphology and the crystalline structure of the composite were similar to that of BC paper as examined by scanning electron microscopy and X-ray diffraction, respectively. Regarding operational properties, specific activities of immobilized and free lysozymewere similar. Moreover, immobilized enzyme showed a broaderworking temperature and higher thermal stability. The composites maintained its activity for at least 80 dayswithout any special storage. Lysozyme-BC paper displayed antimicrobial activity against Gram-positive and Gram-negative bacteria, inhibiting their growth by 82% and 68%, respectively. Additionally, the presence of lysozyme increased the antioxidant activity of BC paper by 30%. The results indicated that BC is a suitable material to produce bioactive paper as it provides a biocompatible environment without compromising the activity of the immobilized protein. BC paper with antimicrobial and antioxidant properties may have application in the field of active packaging.Elsevier B.V.2020202120202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion8 p.application/pdfhttps://hdl.handle.net/2445/164756Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.ijbiomac.2020.04.234International Journal of Biological Macromolecules, 2020, vol. 158, p. 587-594https://doi.org/10.1016/j.ijbiomac.2020.04.234cc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1647562026-05-29T05:05:01Z
dc.title.none.fl_str_mv Development of an antimicrobial bioactive paper made from bacterial cellulose
title Development of an antimicrobial bioactive paper made from bacterial cellulose
spellingShingle Development of an antimicrobial bioactive paper made from bacterial cellulose
Buruaga Ramiro, Carolina
Cel·lulosa
Microbiologia
Fabricació del paper
Cellulose
Microbiology
Papermaking
title_short Development of an antimicrobial bioactive paper made from bacterial cellulose
title_full Development of an antimicrobial bioactive paper made from bacterial cellulose
title_fullStr Development of an antimicrobial bioactive paper made from bacterial cellulose
title_full_unstemmed Development of an antimicrobial bioactive paper made from bacterial cellulose
title_sort Development of an antimicrobial bioactive paper made from bacterial cellulose
dc.creator.none.fl_str_mv Buruaga Ramiro, Carolina
Valenzuela Mayorga, Susana Valeria
Valls Vidal, Cristina
Roncero, M. Blanca
Pastor Blasco, Francisco I. Javier
Díaz Lucea, Pilar
Martínez Martínez, Josefina
author Buruaga Ramiro, Carolina
author_facet Buruaga Ramiro, Carolina
Valenzuela Mayorga, Susana Valeria
Valls Vidal, Cristina
Roncero, M. Blanca
Pastor Blasco, Francisco I. Javier
Díaz Lucea, Pilar
Martínez Martínez, Josefina
author_role author
author2 Valenzuela Mayorga, Susana Valeria
Valls Vidal, Cristina
Roncero, M. Blanca
Pastor Blasco, Francisco I. Javier
Díaz Lucea, Pilar
Martínez Martínez, Josefina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cel·lulosa
Microbiologia
Fabricació del paper
Cellulose
Microbiology
Papermaking
topic Cel·lulosa
Microbiologia
Fabricació del paper
Cellulose
Microbiology
Papermaking
description Bacterial cellulose (BC) has emerged as an attractive adsorptive material for antimicrobial agents due to its fine network structure, its large surface area, and its high porosity. In the present study, BC paper was first produced and then lysozymewas immobilized onto it by physical adsorption, obtaining a composite of lysozyme-BC paper. The morphology and the crystalline structure of the composite were similar to that of BC paper as examined by scanning electron microscopy and X-ray diffraction, respectively. Regarding operational properties, specific activities of immobilized and free lysozymewere similar. Moreover, immobilized enzyme showed a broaderworking temperature and higher thermal stability. The composites maintained its activity for at least 80 dayswithout any special storage. Lysozyme-BC paper displayed antimicrobial activity against Gram-positive and Gram-negative bacteria, inhibiting their growth by 82% and 68%, respectively. Additionally, the presence of lysozyme increased the antioxidant activity of BC paper by 30%. The results indicated that BC is a suitable material to produce bioactive paper as it provides a biocompatible environment without compromising the activity of the immobilized protein. BC paper with antimicrobial and antioxidant properties may have application in the field of active packaging.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/164756
url https://hdl.handle.net/2445/164756
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.ijbiomac.2020.04.234
International Journal of Biological Macromolecules, 2020, vol. 158, p. 587-594
https://doi.org/10.1016/j.ijbiomac.2020.04.234
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425765688279040
score 15,81155

Similares en LA Referencia