Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors

Cellular production of ribosomes involves the formation of highly defined interactions between ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs). Moreover in eukaryotic cells, efficient ribosome maturation requires the transient association of a large number of ribosome biogenesis factors (...

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Autores: Ohmayer, Uli, Gil-Hernández, Álvaro, Sauert, Martina, Martín-Marcos, Pilar, Tamame, Mercedes, Tschochner, Herbert, Griesenbeck, Joachim, Milkereit, Philipp
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/156882
Acceso en línea:http://hdl.handle.net/10261/156882
Access Level:acceso abierto
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spelling Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursorsOhmayer, UliGil-Hernández, ÁlvaroSauert, MartinaMartín-Marcos, PilarTamame, MercedesTschochner, HerbertGriesenbeck, JoachimMilkereit, PhilippCellular production of ribosomes involves the formation of highly defined interactions between ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs). Moreover in eukaryotic cells, efficient ribosome maturation requires the transient association of a large number of ribosome biogenesis factors (RBFs) with newly forming ribosomal subunits. Here, we investigated how r-protein assembly events in the large ribosomal subunit (LSU) rRNA domain II are coordinated with each other and with the association of RBFs in early LSU precursors of the yeast Saccharomyces cerevisiae. Specific effects on the pre-ribosomal association of RBFs could be observed in yeast mutants blocked in LSU rRNA domain II assembly. Moreover, formation of a cluster of r-proteins was identified as a downstream event in LSU rRNA domain II assembly. We analyzed in more detail the functional relevance of eukaryote specific bridges established by this r-protein cluster between LSU rRNA domain II and VI and discuss how they can support the stabilization and efficient processing of yeast early LSU precursor RNAs.This work was supported by a grant which was given in the collaborative research center SFB 960, from the >Deutsche Forschungsgemeinschaft> (www.dfg.de)to JG, HT and PM. AGH acknowledges support through a CSIC JAE Predoc Fellowship from the Spanish Research Council CSIC and an EMBO Short Term Fellowship (ASTF 266-2013). PMM and MT were supported in part by project IPT-2012-1321-06000, granted by the Spanish Ministry of Economy and Competitiveness (MINECO).Peer ReviewedPublic Library of ScienceGerman Research FoundationConsejo Superior de Investigaciones Científicas (España)EMBOMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/156882reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1371/journal.pone.0143768Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1568822026-05-22T06:33:51Z
dc.title.none.fl_str_mv Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
title Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
spellingShingle Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
Ohmayer, Uli
title_short Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
title_full Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
title_fullStr Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
title_full_unstemmed Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
title_sort Studies on the coordination of ribosomal protein assembly events involved in processing and stabilization of yeast early large ribosomal subunit precursors
dc.creator.none.fl_str_mv Ohmayer, Uli
Gil-Hernández, Álvaro
Sauert, Martina
Martín-Marcos, Pilar
Tamame, Mercedes
Tschochner, Herbert
Griesenbeck, Joachim
Milkereit, Philipp
author Ohmayer, Uli
author_facet Ohmayer, Uli
Gil-Hernández, Álvaro
Sauert, Martina
Martín-Marcos, Pilar
Tamame, Mercedes
Tschochner, Herbert
Griesenbeck, Joachim
Milkereit, Philipp
author_role author
author2 Gil-Hernández, Álvaro
Sauert, Martina
Martín-Marcos, Pilar
Tamame, Mercedes
Tschochner, Herbert
Griesenbeck, Joachim
Milkereit, Philipp
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv German Research Foundation
Consejo Superior de Investigaciones Científicas (España)
EMBO
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Cellular production of ribosomes involves the formation of highly defined interactions between ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs). Moreover in eukaryotic cells, efficient ribosome maturation requires the transient association of a large number of ribosome biogenesis factors (RBFs) with newly forming ribosomal subunits. Here, we investigated how r-protein assembly events in the large ribosomal subunit (LSU) rRNA domain II are coordinated with each other and with the association of RBFs in early LSU precursors of the yeast Saccharomyces cerevisiae. Specific effects on the pre-ribosomal association of RBFs could be observed in yeast mutants blocked in LSU rRNA domain II assembly. Moreover, formation of a cluster of r-proteins was identified as a downstream event in LSU rRNA domain II assembly. We analyzed in more detail the functional relevance of eukaryote specific bridges established by this r-protein cluster between LSU rRNA domain II and VI and discuss how they can support the stabilization and efficient processing of yeast early LSU precursor RNAs.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/156882
url http://hdl.handle.net/10261/156882
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1371/journal.pone.0143768
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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