Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.

Thrombospondin 1 (TSP-1), an anti-angiogenic factor and TGF-β activity regulator, has been recently recognized as an adipokine that correlates with obesity, inflammation and insulin-resistance processes. In the present study, epididymal adipocytes of rats that were fed a chow (C) or a high fat diet...

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Detalles Bibliográficos
Autores: Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3, Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31b, Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb, Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82, Portillo, M.P. (María P.)|||/items/a9b050ad-eb19-4d20-8574-d4b2da33c873, Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131, Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/19981
Acceso en línea:https://hdl.handle.net/10171/19981
Access Level:acceso abierto
Palabra clave:Diet-induced obesity
Adipokine
Cell culture
Adipose tissue
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spelling Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31bMilagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebMoreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82Portillo, M.P. (María P.)|||/items/a9b050ad-eb19-4d20-8574-d4b2da33c873Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817cDiet-induced obesityAdipokineCell cultureAdipose tissueThrombospondin 1 (TSP-1), an anti-angiogenic factor and TGF-β activity regulator, has been recently recognized as an adipokine that correlates with obesity, inflammation and insulin-resistance processes. In the present study, epididymal adipocytes of rats that were fed a chow (C) or a high fat diet (HFD) for 50 days, were isolated and incubated (24-72 h) in low (LG; 5.6 mM) or high (HG; 25 mM) glucose, in presence or absence of 1.6 nM insulin. Rats fed the HF diet showed an established obesity state. Serum TSP-1 levels and TSP-1 mRNA basal expression of adipocytes from HFD rats were higher than those from controls. Adipocytes from HFD animals presented an insulin-resistance state, as suggested by the lower insulin-stimulated glucose uptake as compared to controls. TSP-1 expression in culture was higher in adipocytes from obese animals at 24 h, but when the adipocytes were treated with HG, these expression levels dropped dramatically. Later at 72 h, TSP-1 expression was lower in adipocytes from HFD rats, and no effects of the other treatments were observed. Surprisingly, the secretion levels of this protein at 72 h were increased significantly by the HG treatment in both types of adipocytes, although they were even higher in adipocytes from obese animals. Finally, cell viability was significantly reduced by HG treatment in both types of adipocytes. In summary, TSP-1 expression/secretion was modulated in an in vitro model of insulin-resistant adipocytes. The difference between expression and secretion patterns suggests a post-transcriptional regulation. The present study confirms that TPS-1 is closely associated with obesity-related mechanisms.SpringerDadun. Depósito Académico Digital Universidad de Navarra20112011-11-2820112011-01-0120112011-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/19981reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/199812026-06-21T12:47:57Z
dc.title.none.fl_str_mv Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
title Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
spellingShingle Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3
Diet-induced obesity
Adipokine
Cell culture
Adipose tissue
title_short Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
title_full Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
title_fullStr Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
title_full_unstemmed Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
title_sort Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.
dc.creator.none.fl_str_mv Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3
Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31b
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Portillo, M.P. (María P.)|||/items/a9b050ad-eb19-4d20-8574-d4b2da33c873
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
author Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3
author_facet Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3
Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31b
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Portillo, M.P. (María P.)|||/items/a9b050ad-eb19-4d20-8574-d4b2da33c873
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
author_role author
author2 Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31b
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Portillo, M.P. (María P.)|||/items/a9b050ad-eb19-4d20-8574-d4b2da33c873
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Diet-induced obesity
Adipokine
Cell culture
Adipose tissue
topic Diet-induced obesity
Adipokine
Cell culture
Adipose tissue
description Thrombospondin 1 (TSP-1), an anti-angiogenic factor and TGF-β activity regulator, has been recently recognized as an adipokine that correlates with obesity, inflammation and insulin-resistance processes. In the present study, epididymal adipocytes of rats that were fed a chow (C) or a high fat diet (HFD) for 50 days, were isolated and incubated (24-72 h) in low (LG; 5.6 mM) or high (HG; 25 mM) glucose, in presence or absence of 1.6 nM insulin. Rats fed the HF diet showed an established obesity state. Serum TSP-1 levels and TSP-1 mRNA basal expression of adipocytes from HFD rats were higher than those from controls. Adipocytes from HFD animals presented an insulin-resistance state, as suggested by the lower insulin-stimulated glucose uptake as compared to controls. TSP-1 expression in culture was higher in adipocytes from obese animals at 24 h, but when the adipocytes were treated with HG, these expression levels dropped dramatically. Later at 72 h, TSP-1 expression was lower in adipocytes from HFD rats, and no effects of the other treatments were observed. Surprisingly, the secretion levels of this protein at 72 h were increased significantly by the HG treatment in both types of adipocytes, although they were even higher in adipocytes from obese animals. Finally, cell viability was significantly reduced by HG treatment in both types of adipocytes. In summary, TSP-1 expression/secretion was modulated in an in vitro model of insulin-resistant adipocytes. The difference between expression and secretion patterns suggests a post-transcriptional regulation. The present study confirms that TPS-1 is closely associated with obesity-related mechanisms.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-11-28
2011
2011-01-01
2011
2011-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/19981
url https://hdl.handle.net/10171/19981
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
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