Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy

Background: Loa loa is a filarial species found exclusively in West and Central Africa. Microscopy is the traditional diagnosis method for human loiasis. Several molecular methods have developed as an alternative approach for identification of L. loa filarial parasites. Objectives: The aim of this s...

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Autores: Ta Tang, Thuy-Huong, Berzosa, Pedro, Rubio Gonzalez, Jose Miguel, Romay-Barja, Maria, Ncogo, Policarpo, Agudo, Diego, Herrador, Zaida, Cerrada-Gálvez, Laura, Benito, Agustin
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/14449
Acceso en línea:http://hdl.handle.net/20.500.12105/14449
Access Level:acceso abierto
Palabra clave:Loiasis
Humans
Microscopy
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Real-Time Polymerase Chain Reaction
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spelling Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopyTa Tang, Thuy-HuongBerzosa, PedroRubio Gonzalez, Jose MiguelRomay-Barja, MariaNcogo, PolicarpoAgudo, DiegoHerrador, ZaidaCerrada-Gálvez, LauraBenito, AgustinLoiasisHumansMicroscopyMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesReal-Time Polymerase Chain ReactionBackground: Loa loa is a filarial species found exclusively in West and Central Africa. Microscopy is the traditional diagnosis method for human loiasis. Several molecular methods have developed as an alternative approach for identification of L. loa filarial parasites. Objectives: The aim of this study was to evaluate a Loa-Loop-mediated isothermal amplification (LAMP) assay to diagnose loiasis disease on dried blood spots (DBS) samples, compared to microscopy, filaria-real time-polymerase chain reaction (PCR) and nested-Loa PCR. Methods: A total of 100 DBS samples and 100 blood smears were used for this study. DNA was extracted using saponin/Chelex method. DNA isolated was assayed by a Loa-LAMP assay in parallel to microscopy, filaria-real time PCR and nested-Loa PCR. The sensitivities and specificities of Loa-LAMP assay was computed comparing to each one of the reference methods. Findings: Loa-LAMP's sensitivity was more than 90% and specificity was nearly 100% when compared to molecular methods. On the other hand, sensitivity was decreased a bit when Loa-LAMP faced microscopy, but keeping the other statistical values high. Main conclusions: Loa-LAMP is an appropriate method for loiasis diagnosis in endemic areas. Though, it has disadvantages like the reagents' high price at the moment and not to be able to detect more filarial species at once.Fundação Oswaldo Cruz (Fiocruz)Instituto de Salud Carlos IIIRETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)20222022-05-2320222022-02-1120222022-02-11research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/14449reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/144492026-06-12T12:43:37Z
dc.title.none.fl_str_mv Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
title Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
spellingShingle Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
Ta Tang, Thuy-Huong
Loiasis
Humans
Microscopy
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Real-Time Polymerase Chain Reaction
title_short Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
title_full Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
title_fullStr Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
title_full_unstemmed Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
title_sort Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
dc.creator.none.fl_str_mv Ta Tang, Thuy-Huong
Berzosa, Pedro
Rubio Gonzalez, Jose Miguel
Romay-Barja, Maria
Ncogo, Policarpo
Agudo, Diego
Herrador, Zaida
Cerrada-Gálvez, Laura
Benito, Agustin
author Ta Tang, Thuy-Huong
author_facet Ta Tang, Thuy-Huong
Berzosa, Pedro
Rubio Gonzalez, Jose Miguel
Romay-Barja, Maria
Ncogo, Policarpo
Agudo, Diego
Herrador, Zaida
Cerrada-Gálvez, Laura
Benito, Agustin
author_role author
author2 Berzosa, Pedro
Rubio Gonzalez, Jose Miguel
Romay-Barja, Maria
Ncogo, Policarpo
Agudo, Diego
Herrador, Zaida
Cerrada-Gálvez, Laura
Benito, Agustin
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)
dc.subject.none.fl_str_mv Loiasis
Humans
Microscopy
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Real-Time Polymerase Chain Reaction
topic Loiasis
Humans
Microscopy
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Real-Time Polymerase Chain Reaction
description Background: Loa loa is a filarial species found exclusively in West and Central Africa. Microscopy is the traditional diagnosis method for human loiasis. Several molecular methods have developed as an alternative approach for identification of L. loa filarial parasites. Objectives: The aim of this study was to evaluate a Loa-Loop-mediated isothermal amplification (LAMP) assay to diagnose loiasis disease on dried blood spots (DBS) samples, compared to microscopy, filaria-real time-polymerase chain reaction (PCR) and nested-Loa PCR. Methods: A total of 100 DBS samples and 100 blood smears were used for this study. DNA was extracted using saponin/Chelex method. DNA isolated was assayed by a Loa-LAMP assay in parallel to microscopy, filaria-real time PCR and nested-Loa PCR. The sensitivities and specificities of Loa-LAMP assay was computed comparing to each one of the reference methods. Findings: Loa-LAMP's sensitivity was more than 90% and specificity was nearly 100% when compared to molecular methods. On the other hand, sensitivity was decreased a bit when Loa-LAMP faced microscopy, but keeping the other statistical values high. Main conclusions: Loa-LAMP is an appropriate method for loiasis diagnosis in endemic areas. Though, it has disadvantages like the reagents' high price at the moment and not to be able to detect more filarial species at once.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-05-23
2022
2022-02-11
2022
2022-02-11
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/14449
url http://hdl.handle.net/20.500.12105/14449
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz (Fiocruz)
publisher.none.fl_str_mv Fundação Oswaldo Cruz (Fiocruz)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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