PR-LncRNA signature regulates glioma cell activity through expression of SOX factors

Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs (PR-LncRN...

Descripción completa

Detalles Bibliográficos
Autores: Torres-Bayona, S. (Sergio)|||/items/033938f1-bff1-4519-8a67-6fd58033dff0, Aldaz, P. (Paula)|||/items/b9c0f230-0f8e-46a0-94e8-b85079ff62d9, Auzmendi-Iriarte, J. (Jaione)|||/items/9bd1c422-5726-4500-bab1-1a65f7248f7f, Saenz-Antoñanzas, A. (Ander)|||/items/0fc8b002-19bb-47ca-9df2-93984b896adc, Garcia, I. (Idoia)|||/items/f25ad8fd-f159-41fe-b004-686308dbd227, Arrazola, M. (Mariano)|||/items/66ec93ca-56c1-45d0-8d4d-c18d3b0382ad, Gerovska, D. (Daniela)|||/items/9732e088-1235-4044-80b1-fe1603a5912e, Undabeitia, J. (Jose)|||/items/13a5ed52-5270-478a-88a3-2d026a956a9d, Querejeta, A. (Arrate)|||/items/2d8e5cd9-5b00-45bc-8f2c-89fede837e6b, Egaña, L. (Larraitz)|||/items/69ab62f6-88da-4a8a-b602-e854d2c96b73, Villanúa, J. (Jorge)|||/items/7e7a73b0-8602-480e-9173-d74990cf90e5, Ruiz, I. (Irune)|||/items/a578bec2-91d8-4654-a70a-9946e46b57ff, Sarasqueta, C. (Cristina)|||/items/fca900e8-828b-4a13-9038-f6b7e0fc9a78, Urculo, E. (Enrique)|||/items/38200038-0a9b-4ac7-bc43-f698d9f416ba, Araúzo-Bravo, M.J. (Marcos J.)|||/items/0e22ab9e-20ca-4a9e-b11f-7fd8fa9027d8, Huarte-Martínez, M. (Maite)|||/items/db8d6b5d-dfc8-45d6-b87f-dd89deeded17, Sampron, N. (Nicolas)|||/items/15bfc1f7-2887-4680-8f15-97d782d6328b, Matheu, A. (Ander)|||/items/79d399df-1789-495b-af31-49af2b5ea9e0
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/65674
Acceso en línea:https://hdl.handle.net/10171/65674
Access Level:acceso abierto
Palabra clave:Long non-coding RNAs (LncRNAs)
Biological processes
Tumor suppressor
Descripción
Sumario:Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs (PR-LncRNAs) in colorectal cancer. Our aim was to identify the expression and function of this signature in gliomas. We found that the expression of the four PR-LncRNAs tested was high in human low-grade glioma samples and diminished with increasing grade of disease, being the lowest in glioblastoma samples. Functional assays demonstrated that PR-LncRNA silencing increased glioma cell proliferation and oncosphere formation. Mechanistically, we found an inverse correlation between PR-LncRNA expression and SOX1, SOX2 and SOX9 stem cell factors in human glioma biopsies and in glioma cells in vitro. Moreover, knock-down of SOX activity abolished the effect of PR-LncRNA silencing in glioma cell activity. In conclusion, our results demonstrate that the expression and function of PR-LncRNAs are significantly altered in gliomagenesis and that their activity is mediated by SOX factors. These results may provide important insights into the mechanisms responsible for glioblastoma pathogenesis.

Similares en LA Referencia