Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability

Gene silencing; Quasispecies; Ribozyme

Detalles Bibliográficos
Autores: Pacín Ruíz, Beatriz, Tabernero Caellas, David, Sopena Santisteve, Sara, Garcia Garcia, Selene, Casillas Gomez, Rosario, Najarro Centeno, Adrián, Aldama Bañares, Unai, Palom Agusti, Adriana, Galan González, Anna, Vila Salvador, Marta, Riveiro Barciela, Maria del Mar, Quer Sivila, Josep, Rodríguez Frias, Francisco, Rando Segura, Ariadna, Gregori Font, Josep, Cortese, Maria Francesca, Buti Ferret, Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/7880
Acceso en línea:https://hdl.handle.net/11351/7880
Access Level:acceso abierto
Palabra clave:Genotip
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
id ES_fe5b794f1a38e4f9f1406fcab7d08c4e
oai_identifier_str oai:recercat.cat:11351/7880
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
title Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
spellingShingle Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
Pacín Ruíz, Beatriz
Genotip
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
title_short Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
title_full Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
title_fullStr Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
title_full_unstemmed Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
title_sort Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
dc.creator.none.fl_str_mv Pacín Ruíz, Beatriz
Tabernero Caellas, David
Sopena Santisteve, Sara
Garcia Garcia, Selene
Casillas Gomez, Rosario
Najarro Centeno, Adrián
Aldama Bañares, Unai
Palom Agusti, Adriana
Galan González, Anna
Vila Salvador, Marta
Riveiro Barciela, Maria del Mar
Quer Sivila, Josep
Rodríguez Frias, Francisco
Rando Segura, Ariadna
Gregori Font, Josep
Cortese, Maria Francesca
Buti Ferret, Maria
author Pacín Ruíz, Beatriz
author_facet Pacín Ruíz, Beatriz
Tabernero Caellas, David
Sopena Santisteve, Sara
Garcia Garcia, Selene
Casillas Gomez, Rosario
Najarro Centeno, Adrián
Aldama Bañares, Unai
Palom Agusti, Adriana
Galan González, Anna
Vila Salvador, Marta
Riveiro Barciela, Maria del Mar
Quer Sivila, Josep
Rodríguez Frias, Francisco
Rando Segura, Ariadna
Gregori Font, Josep
Cortese, Maria Francesca
Buti Ferret, Maria
author_role author
author2 Tabernero Caellas, David
Sopena Santisteve, Sara
Garcia Garcia, Selene
Casillas Gomez, Rosario
Najarro Centeno, Adrián
Aldama Bañares, Unai
Palom Agusti, Adriana
Galan González, Anna
Vila Salvador, Marta
Riveiro Barciela, Maria del Mar
Quer Sivila, Josep
Rodríguez Frias, Francisco
Rando Segura, Ariadna
Gregori Font, Josep
Cortese, Maria Francesca
Buti Ferret, Maria
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Pacin-Ruiz B, Cortese MF, Tabernero D, García-García S] Unitat d’Hepatologia, Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain. [Sopena S, Casillas R, Najarro A, Aldama U, Galán A, Vila M] Unitat d’Hepatologia, Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Gregori J, Quer J] Unitat del Fetge, Laboratori de Malalties Hepàtiques, Vall d’Hebron Institut Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Palom A] Unitat del Fetge, Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rando-Segura A] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Riveiro-Barciela M, Buti M] Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain. Unitat del Fetge, Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rodríguez-Frías F] Unitat d’Hepatologia, Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain. Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Genotip
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
topic Genotip
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
description Gene silencing; Quasispecies; Ribozyme
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/7880
url https://hdl.handle.net/11351/7880
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Viruses;14(2)
https://doi.org/10.3390/v14020215
info:eu-repo/grantAgreement/ES/PE2017-2020/PI20%2F01692
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425677960216576
spelling Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and VariabilityPacín Ruíz, BeatrizTabernero Caellas, DavidSopena Santisteve, SaraGarcia Garcia, SeleneCasillas Gomez, RosarioNajarro Centeno, AdriánAldama Bañares, UnaiPalom Agusti, AdrianaGalan González, AnnaVila Salvador, MartaRiveiro Barciela, Maria del MarQuer Sivila, JosepRodríguez Frias, FranciscoRando Segura, AriadnaGregori Font, JosepCortese, Maria FrancescaButi Ferret, MariaGenotipHepatitis vírica - Aspectes genèticsORGANISMS::Viruses::Defective Viruses::Hepatitis Delta VirusOther subheadings::Other subheadings::Other subheadings::/geneticsORGANISMOS::virus::virus defectuosos::virus de la hepatitis deltaOtros calificadores::Otros calificadores::Otros calificadores::/genéticaGene silencing; Quasispecies; RibozymeSilenciament gènic; Quasiespècie; RibozimaSilenciamiento de genes; Cuasiespecies; RibozimaThe hepatitis delta virus (HDV) genome has an autocatalytic region called the ribozyme, which is essential for viral replication. The aim of this study was to use next-generation sequencing (NGS) to analyze the ribozyme quasispecies (QS) in order to study its evolution and identify highly conserved regions potentially suitable for a gene-silencing strategy. HDV RNA was extracted from 2 longitudinal samples of chronic HDV patients and the ribozyme (nucleotide, nt 688–771) was analyzed using NGS. QS conservation, variability and genetic distance were analyzed. Mutations were identified by aligning sequences with their specific genotype consensus. The main relevant mutations were tested in vitro. The ribozyme was conserved overall, with a hyper-conserved region between nt 715–745. No difference in QS was observed over time. The most variable region was between nt 739–769. Thirteen mutations were observed, with three showing a higher frequency: T23C, T69C and C64 deletion. This last strongly reduced HDV replication by more than 1 log in vitro. HDV Ribozyme QS was generally highly conserved and was maintained during follow-up. The most conserved portion may be a valuable target for a gene-silencing strategy. The presence of the C64 deletion may strongly impair viral replication, as it is a potential mechanism of viral persistence.This research was funded by Institute of Health Carlos III, grant number PI20/01692 and co-financed by the European Regional Development Fund (ERDF), and by the European Regional Development Fund (ERDF)- Ministry of Economy, Industry and Competitiveness, grantRTI2018-101936-B-I00.MDPIInstitut Català de la Salut[Pacin-Ruiz B, Cortese MF, Tabernero D, García-García S] Unitat d’Hepatologia, Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain. [Sopena S, Casillas R, Najarro A, Aldama U, Galán A, Vila M] Unitat d’Hepatologia, Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Gregori J, Quer J] Unitat del Fetge, Laboratori de Malalties Hepàtiques, Vall d’Hebron Institut Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Palom A] Unitat del Fetge, Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rando-Segura A] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Riveiro-Barciela M, Buti M] Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain. Unitat del Fetge, Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rodríguez-Frías F] Unitat d’Hepatologia, Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain. Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, SpainVall d'Hebron Barcelona Hospital Campus202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11351/7880Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésViruses;14(2)https://doi.org/10.3390/v14020215info:eu-repo/grantAgreement/ES/PE2017-2020/PI20%2F01692Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/78802026-05-29T05:05:01Z
score 15,81155

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