Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212

15 Pág. Departamento de ​Protección Vegetal

Detalles Bibliográficos
Autores: Requena, Elena, Alonso-Guirado, Lola, Veloso, Javier, Villarino, María, Melgarejo, Paloma, Espeso, Eduardo A., Larena, Inmaculada
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/296230
Acceso en línea:http://hdl.handle.net/10261/296230
https://api.elsevier.com/content/abstract/scopus_id/85147170819
Access Level:acceso abierto
Palabra clave:PO212
Penicillium rubens
Biocontrol agent
Genome
Comparative genomics
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spelling Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212Requena, ElenaAlonso-Guirado, LolaVeloso, JavierVillarino, MaríaMelgarejo, PalomaEspeso, Eduardo A.Larena, InmaculadaPO212Penicillium rubensBiocontrol agentGenomeComparative genomics15 Pág. Departamento de ​Protección VegetalPenicillium rubens strain 212 (PO212) is a filamentous fungus belonging to the division Ascomycete. PO212 acts as an effective biocontrol agent against several pathogens in a variety of horticultural crops including Fusarium oxysporum f.sp. lycopersici, causing vascular wilt disease in tomato plants. We assembled draft genomes of two P. rubens strains, the biocontrol agent PO212 and the soil isolate S27, which lacks biocontrol activity. We also performed comparative analyses of the genomic sequence of PO212 with that of the other P. rubens and P. chrysogenum strains. This is the first Penicillium strain with biocontrol activity whose genome has been sequenced and compared. PO212 genome size is 2,982 Mb, which is currently organized into 65 scaffolds and a total of 10,164 predicted Open Reading Frames (ORFs). Sequencing confirmed that PO212 belongs to P. rubens clade. The comparative analysis of the PO212 genome with the genomes of other P. rubens and Penicillium chrysogenum strains available in databases showed strong conservation among genomes, but a correlation was not found between these genomic data and the biocontrol phenotype displayed by PO212. Finally, the comparative analysis between PO212 and S27 genomes showed high sequence conservation and a low number of variations mainly located in ORF regions. These differences found in coding regions between PO212 and S27 genomes can explain neither the biocontrol activity of PO212 nor the absence of such activity in S27, opening a possible avenue toward transcriptomic and epigenetic studies that may shed light on this mechanism for fighting plant diseases caused by fungal pathogens. The genome sequences described in this study provide a useful novel resource for future research into the biology, ecology, and evolution of biological control agents.The work at the INIA-CSIC laboratory was supported by the RTA2013-00060-C05-01 (Plan Nacional de Ministerio de Economía y Competitividad), RTA2017-00019-C03-01 (Plan Nacional de I + D, MICIU, Spain), and PID2021-123594OR-C21 (MCIN/AEI/10.13039/501100011033/FEDER, UE). ER received a scholarship from the MICIU.Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionRequena, Elena [0000-0001-5028-8880]Alonso-Guirado, Lola [0000-0002-3493-718X]Veloso, Javier [0000-0002-7283-769X]Villarino, María [0000-0002-5635-8146]Espeso, Eduardo A. [0000-0002-5873-6059]Larena, Inmaculada [0000-0001-8424-8916]Melgarejo, Paloma [0000-0002-3698-8896]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/296230https://api.elsevier.com/content/abstract/scopus_id/85147170819reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//RTA2013-00060-C05-01info:eu-repo/grantAgreement/AEI//RTA2017-00019-C03-01info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-123594OR-C21Frontiers in microbiologyhttps://dx.doi.org/10.3389/fmicb.2022.1075327Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2962302026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
title Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
spellingShingle Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
Requena, Elena
PO212
Penicillium rubens
Biocontrol agent
Genome
Comparative genomics
title_short Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
title_full Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
title_fullStr Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
title_full_unstemmed Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
title_sort Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
dc.creator.none.fl_str_mv Requena, Elena
Alonso-Guirado, Lola
Veloso, Javier
Villarino, María
Melgarejo, Paloma
Espeso, Eduardo A.
Larena, Inmaculada
author Requena, Elena
author_facet Requena, Elena
Alonso-Guirado, Lola
Veloso, Javier
Villarino, María
Melgarejo, Paloma
Espeso, Eduardo A.
Larena, Inmaculada
author_role author
author2 Alonso-Guirado, Lola
Veloso, Javier
Villarino, María
Melgarejo, Paloma
Espeso, Eduardo A.
Larena, Inmaculada
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Requena, Elena [0000-0001-5028-8880]
Alonso-Guirado, Lola [0000-0002-3493-718X]
Veloso, Javier [0000-0002-7283-769X]
Villarino, María [0000-0002-5635-8146]
Espeso, Eduardo A. [0000-0002-5873-6059]
Larena, Inmaculada [0000-0001-8424-8916]
Melgarejo, Paloma [0000-0002-3698-8896]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PO212
Penicillium rubens
Biocontrol agent
Genome
Comparative genomics
topic PO212
Penicillium rubens
Biocontrol agent
Genome
Comparative genomics
description 15 Pág. Departamento de ​Protección Vegetal
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/296230
https://api.elsevier.com/content/abstract/scopus_id/85147170819
url http://hdl.handle.net/10261/296230
https://api.elsevier.com/content/abstract/scopus_id/85147170819
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//RTA2013-00060-C05-01
info:eu-repo/grantAgreement/AEI//RTA2017-00019-C03-01
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-123594OR-C21
Frontiers in microbiology
https://dx.doi.org/10.3389/fmicb.2022.1075327
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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