Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna

Background: The analysis of obesogenic effects in invertebrates is limited by our poor knowledge of the regulatory pathways of lipid metabolism. Recent data from the crustacean Daphnia magna points to three signaling hormonal pathways related to the molting and reproductive cycles [retinoic X recept...

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Autores: Jordão, Rita, Casas, Josefina, Fabriàs, Gemma, Campos, B., Piña, Benjamín, Lemos, Marco F. L., Soares, Amadeu M. V. M., Tauler, Romà, Barata Martí, Carlos
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/128101
Acceso en línea:http://hdl.handle.net/10261/128101
Access Level:acceso abierto
Palabra clave:Daphnia magna
lipid composition
time of flight mass spectrometry
ultra performance liquid chromatography
glycerophospholipid
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spelling Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magnaJordão, RitaCasas, JosefinaFabriàs, GemmaCampos, B.Piña, BenjamínLemos, Marco F. L.Soares, Amadeu M. V. M.Tauler, RomàBarata Martí, CarlosDaphnia magnalipid compositiontime of flight mass spectrometryultra performance liquid chromatographyglycerophospholipidBackground: The analysis of obesogenic effects in invertebrates is limited by our poor knowledge of the regulatory pathways of lipid metabolism. Recent data from the crustacean Daphnia magna points to three signaling hormonal pathways related to the molting and reproductive cycles [retinoic X receptor (RXR), juvenile hormone (JH), and ecdysone] as putative targets for exogenous obesogens. Objective: The present study addresses the disruptive effects of the model obesogen tributyltin (TBT) on the lipid homeostasis in Daphnia during the molting and reproductive cycle, its genetic control, and health consequences of its disruption. Methods: D. magna individuals were exposed to low and high levels of TBT. Reproductive effects were assessed by Life History analysis methods. Quantitative and qualitative changes in lipid droplets during molting and the reproductive cycle were studied using Nile red staining. Lipid composition and dynamics were analyzed by ultra-performance liquid chromatography coupled to a time-of-flight mass spectrometer. Relative abundances of mRNA from different genes related to RXR, ecdysone, and JH signaling pathways were studied by qRT-PCR. Results and Conclusions: TBT disrupted the dynamics of neutral lipids, impairing the transfer of triacylglycerols to eggs and hence promoting their accumulation in adult individuals. TBT’s disruptive effects translated into a lower fitness for offspring and adults. Co-regulation of gene transcripts suggests that TBT activates the ecdysone, JH, and RXR receptor signaling pathways, presumably through the already proposed interaction with RXR. These findings indicate the presence of obesogenic effects in a nonvertebrate species.This project was supported by the Spanish MEC CTM2011-30471-C02-01and Advance grant –EU grants ERC-2012-AdG-320737. The Portuguese Foundation for Science and Technology (FCT), supported the doctoral fellowship of Rita Jordão (SFRH/BD/79453/2011).Peer reviewedDepartment of Health and Human Services (U.S.)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/128101reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/320737DOI: 10.1289/ehp.1409163Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1281012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
title Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
spellingShingle Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
Jordão, Rita
Daphnia magna
lipid composition
time of flight mass spectrometry
ultra performance liquid chromatography
glycerophospholipid
title_short Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
title_full Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
title_fullStr Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
title_full_unstemmed Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
title_sort Obesogens beyond vertebrates: Lipid perturbation by tributyltin in the crustacean Daphnia magna
dc.creator.none.fl_str_mv Jordão, Rita
Casas, Josefina
Fabriàs, Gemma
Campos, B.
Piña, Benjamín
Lemos, Marco F. L.
Soares, Amadeu M. V. M.
Tauler, Romà
Barata Martí, Carlos
author Jordão, Rita
author_facet Jordão, Rita
Casas, Josefina
Fabriàs, Gemma
Campos, B.
Piña, Benjamín
Lemos, Marco F. L.
Soares, Amadeu M. V. M.
Tauler, Romà
Barata Martí, Carlos
author_role author
author2 Casas, Josefina
Fabriàs, Gemma
Campos, B.
Piña, Benjamín
Lemos, Marco F. L.
Soares, Amadeu M. V. M.
Tauler, Romà
Barata Martí, Carlos
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Daphnia magna
lipid composition
time of flight mass spectrometry
ultra performance liquid chromatography
glycerophospholipid
topic Daphnia magna
lipid composition
time of flight mass spectrometry
ultra performance liquid chromatography
glycerophospholipid
description Background: The analysis of obesogenic effects in invertebrates is limited by our poor knowledge of the regulatory pathways of lipid metabolism. Recent data from the crustacean Daphnia magna points to three signaling hormonal pathways related to the molting and reproductive cycles [retinoic X receptor (RXR), juvenile hormone (JH), and ecdysone] as putative targets for exogenous obesogens. Objective: The present study addresses the disruptive effects of the model obesogen tributyltin (TBT) on the lipid homeostasis in Daphnia during the molting and reproductive cycle, its genetic control, and health consequences of its disruption. Methods: D. magna individuals were exposed to low and high levels of TBT. Reproductive effects were assessed by Life History analysis methods. Quantitative and qualitative changes in lipid droplets during molting and the reproductive cycle were studied using Nile red staining. Lipid composition and dynamics were analyzed by ultra-performance liquid chromatography coupled to a time-of-flight mass spectrometer. Relative abundances of mRNA from different genes related to RXR, ecdysone, and JH signaling pathways were studied by qRT-PCR. Results and Conclusions: TBT disrupted the dynamics of neutral lipids, impairing the transfer of triacylglycerols to eggs and hence promoting their accumulation in adult individuals. TBT’s disruptive effects translated into a lower fitness for offspring and adults. Co-regulation of gene transcripts suggests that TBT activates the ecdysone, JH, and RXR receptor signaling pathways, presumably through the already proposed interaction with RXR. These findings indicate the presence of obesogenic effects in a nonvertebrate species.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/128101
url http://hdl.handle.net/10261/128101
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/320737
DOI: 10.1289/ehp.1409163
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Department of Health and Human Services (U.S.)
publisher.none.fl_str_mv Department of Health and Human Services (U.S.)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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