Identification and characterization of Diaporthe spp. associated with twig cankers and shoot blight of almonds in Spain

[EN] Two hundred and twenty-fiveDiaportheisolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS,tub,tef-1 alpha,calandhis), allowed the identification of...

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Detalhes bibliográficos
Autores: León Santana, Maela|||0000-0003-0693-6988, Berbegal Martinez, Monica|||0000-0002-5773-3104, Rodríguez Reina, José Manuel, Abad Campos, Paloma|||0000-0002-5979-4349, RAMON ALBALAT, ANTONIO, Armengol Fortí, Josep|||0000-0003-3815-8578, Elena, Georgina, Olmo, Diego, Vicent, Antonio, Luque, Jordi, Miarnau, Xavier, Agustí-Brisach, Carlos, Trapero, Antonio, Capote, Nieves, Arroyo, Francisco T., Avilés, Manuel, Gramaje, David, Andrés-Sodupe, Marcos
Tipo de documento: artigo
Data de publicação:2020
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositório:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglês
OAI Identifier:oai:riunet.upv.es:10251/164960
Acesso em linha:https://riunet.upv.es/handle/10251/164960
Access Level:Acceso aberto
Palavra-chave:Diaporthe amygdali
D. mediterranea
Multilocus DNA sequence analysis
Pathogenicity
Prunus dulcis
PRODUCCION VEGETAL
BOTANICA
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
Descrição
Resumo:[EN] Two hundred and twenty-fiveDiaportheisolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS,tub,tef-1 alpha,calandhis), allowed the identification of four knownDiaporthespecies, namely:D. amygdali,D. eres,D. foeniculinaandD. phaseolorum. Moreover, a novel phylogenetic species,D. mediterranea, was described.Diaportheamygdaliwas the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species wasD. foeniculina(10.2%), followed byD. mediterranea(3.6%),D.eresandD. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except forD. foeniculinaandD. phaseolorum, allDiaporthespecies were able to cause lesions significantly different from those developed on the uninoculated controls.Diaporthe mediterraneacaused the most severe symptoms. These results confirmD. amygdalias a key pathogen of almonds in Spain. Moreover, the new species,D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop.

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