Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences

Gold nanoparticles modified with anti-mouse IgG have been used to trace oligonucleotides carrying a c-myc peptide. Two strategies, a dot-blot format as well as inductively coupled plasma mass spectrometry (ICPMS) have been used to detect the nanoparticle tracer. For both cases, oligonucleotide-pepti...

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Autores: Merkoçi, Arben, Aldavert, Marta, Tarrasón, Gema, Eritja Casadellà, Ramón, Alegret, Salvador
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2005
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/136645
Acesso em linha:http://hdl.handle.net/10261/136645
Access Level:acceso abierto
Palavra-chave:Gold nanoparticles
Inductively coupled plasma mass spectrometry (ICPMS)
DNA
Atomic emission spectrometry
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spelling Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequencesMerkoçi, ArbenAldavert, MartaTarrasón, GemaEritja Casadellà, RamónAlegret, SalvadorGold nanoparticlesInductively coupled plasma mass spectrometry (ICPMS)DNAAtomic emission spectrometryGold nanoparticles modified with anti-mouse IgG have been used to trace oligonucleotides carrying a c-myc peptide. Two strategies, a dot-blot format as well as inductively coupled plasma mass spectrometry (ICPMS) have been used to detect the nanoparticle tracer. For both cases, oligonucleotide-peptide conjugates were first applied to a nitrocellulose membrane using a manifold attached to a suction device. After immobilization of the oligonucleotide by UV radiation, the samples were incubated with an anti-c-myc monoclonal antibody. In the case of the dot-blot format strategy, it was followed by incubation with a secondary antibody conjugated to horseradish peroxidase and development with luminol as chemiluminescent substrate. In the case of ICPMS strategy, it was followed by incubation with the secondary antibody (anti-mouse IgG) conjugated to gold nanoparticles and their ICPMS detection after dissolving. The nonspecific adsorptions were found to be around zero. The limit of detection for peptide-modified DNA was 0,2 pmol. The method may have significant potential as an important ICPMS-based nonradioactive DNA detection method for the simultaneous determination of various sequences by labeling different kinds of inorganic nanoparticles. © 2005 American Chemical Society.This work was financially supported from MEC (Madrid) (Projects BIO2004- 02776, MAT2004-05164), by Spanish foundation “Ramón Areces” (project ‘Bionanosensores’). A.M. thanks the “Ramón y Cajal” program of Ministry of Science and Technology (Madrid).Peer reviewedAmerican Chemical SocietyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162005info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/136645reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1021/ac050539lSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1366452026-05-22T06:33:51Z
dc.title.none.fl_str_mv Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
title Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
spellingShingle Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
Merkoçi, Arben
Gold nanoparticles
Inductively coupled plasma mass spectrometry (ICPMS)
DNA
Atomic emission spectrometry
title_short Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
title_full Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
title_fullStr Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
title_full_unstemmed Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
title_sort Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
dc.creator.none.fl_str_mv Merkoçi, Arben
Aldavert, Marta
Tarrasón, Gema
Eritja Casadellà, Ramón
Alegret, Salvador
author Merkoçi, Arben
author_facet Merkoçi, Arben
Aldavert, Marta
Tarrasón, Gema
Eritja Casadellà, Ramón
Alegret, Salvador
author_role author
author2 Aldavert, Marta
Tarrasón, Gema
Eritja Casadellà, Ramón
Alegret, Salvador
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gold nanoparticles
Inductively coupled plasma mass spectrometry (ICPMS)
DNA
Atomic emission spectrometry
topic Gold nanoparticles
Inductively coupled plasma mass spectrometry (ICPMS)
DNA
Atomic emission spectrometry
description Gold nanoparticles modified with anti-mouse IgG have been used to trace oligonucleotides carrying a c-myc peptide. Two strategies, a dot-blot format as well as inductively coupled plasma mass spectrometry (ICPMS) have been used to detect the nanoparticle tracer. For both cases, oligonucleotide-peptide conjugates were first applied to a nitrocellulose membrane using a manifold attached to a suction device. After immobilization of the oligonucleotide by UV radiation, the samples were incubated with an anti-c-myc monoclonal antibody. In the case of the dot-blot format strategy, it was followed by incubation with a secondary antibody conjugated to horseradish peroxidase and development with luminol as chemiluminescent substrate. In the case of ICPMS strategy, it was followed by incubation with the secondary antibody (anti-mouse IgG) conjugated to gold nanoparticles and their ICPMS detection after dissolving. The nonspecific adsorptions were found to be around zero. The limit of detection for peptide-modified DNA was 0,2 pmol. The method may have significant potential as an important ICPMS-based nonradioactive DNA detection method for the simultaneous determination of various sequences by labeling different kinds of inorganic nanoparticles. © 2005 American Chemical Society.
publishDate 2005
dc.date.none.fl_str_mv 2005
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/136645
url http://hdl.handle.net/10261/136645
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1021/ac050539l
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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